199 research outputs found
Hydrogen peroxide treatment and its impacts on Lepeophtheirus salmonis originating from the Bay of Fundy, Canada
No full textHydrogen peroxide (H2O2) is used to treat sea lice infections of farmed salmonids in the Atlantic and Pacific Oceans and issues with resistance to this treatment, and others are a major threat to the sustainability of the industry. The objectives of this study were to determine how H2O2 exposure affects survival and antioxidant‐related gene expression in salmon lice (Lepeophtheirus salmonis) collected from the Bay of Fundy, New Brunswick. The maximum recommended dose of H2O2 is 1,800 mg/L, while the EC50 values (with 95% CI) for the population tested were 1,486 (457, 2,515) mg/L for males and 2,126 (984, 3,268) mg/L for females. Neither temperature nor pretreatment with emamectin benzoate (EMB) impacted survival after H2O2 exposure. RT‐qPCR was performed on pre‐adult sea lice exposed to H2O2 and showed that four genes classically involved in the response to oxidative stress were unchanged between treated and control groups. Seven genes were found to be significantly upregulated in males and one in females. This is the first report on the efficacy and molecular responses of Atlantic Canada sea lice to H2O2 treatment
Augmenting endogenous wnt signaling improves skin wound healing
No full textWnt signaling is required for both the development and homeostasis of the skin, yet its contribution to skin wound repair remains controversial. By employing Axin2(LacZ/+) reporter mice we evaluated the spatial and temporal distribution patterns of Wnt responsive cells, and found that the pattern of Wnt responsiveness varies with the hair cycle, and correlates with wound healing potential. Using Axin2(LacZ/LacZ) mice and an ear wound model, we demonstrate that amplified Wnt signaling leads to improved healing. Utilizing a biochemical approach that mimics the amplified Wnt response of Axin2(LacZ/LacZ) mice, we show that topical application of liposomal Wnt3a to a non-healing wound enhances endogenous Wnt signaling, and results in better skin wound healing. Given the importance of Wnt signaling in the maintenance and repair of skin, liposomal Wnt3a may have widespread application in clinical practice
ELMs and disruptions in ITER: Expected Energy Fluxes on Plasma Facing Components from Multi-machine Experimental Extrapolations and Consequences for ITER Operation
No full textCRPPSPCcont. of author list: G. Maddaluno, D. Whyte, A. Leonard, M. Fenstermacher, R.A. Pitts, I. Landman, B. Bazylev, S. Pestchanyi, A. Zhitlukhin, V.Podkovyrov , N. Klimov, V. Safronov, M. Becoulet, B. Kuteev, V. Koidan, L. Khimchenk
Public worship and practical theology in the work of Benjamin Keach (1640-1704)
No full textThe late seventeenth century was a critical and fruitful period
for the Particular Baptists of England. Severely persecuted following
the Restoration, toleration in 1689 brought its own perils.
Particular Baptists were fortunate in having several strong leaders,
especially the London trio of Hanserd Knollys, William Kiffin, and
Benjamin Keach. Such a small and severely persecuted group as the
Baptists could afford little time for academic pursuits, thus of
necessity most of their theology was practical in nature.
Benjamin Keach (1640-1704) was the most outstanding practical
theologian among the English Particular Baptists of the late
seventeenth century. This dissertation is a study of Keach, in
particular his writings on public worship and practical theology.
Although Keach was a prolific author, he has been almost completely
neglected by scholars.
After a biographical sketch of Keach, this study considers his
writings on public worship and practical theology. In the area of
worship, Keach made two outstanding contributions: First, he was the
most vocal apologist for Baptist views on Baptism of his period.
Secondly, and more importantly, his hymn writing and defense of hymn
singing broke new ground, not just for Baptists, but for English
Protestantism, in general. In addition to his contributions in these
areas, he also dealt with the laying on of hands and the sabbath day
worship controversy.
Keach's contributions to practical theology fall into two main
groups: his writings that concern religious education and those that
deal with polity. In addition to these, Keach's vigorous advocacy of
a high Calvinist soteriology are also considered under the rubric of
practical theology. Keach's most important (although not his most
positive) contribution in this area were his soteriological writings.
Although well within the bounds of orthodoxy, some of the tendencies
in Keach's soteriology were taken up by the following generation of
Baptist leaders and developed into a stultifying hyper-Calvinism that
handicapped Baptist evangelism and missions.
In the conclusion, Keach's contributions to a theory of practical
theology are considered
An assessment of lesions in bay scallops Argopecten irradians attributed to Perkinsus karlssoni (Protozoa, Apicomplexa)
No full textLesions found in bay scallops Argopecten irradians imported into Atlantic Canada for culture were attributed to a parasite Perkinsus karlssoni. It appears, however, that stages from several organisms were combined to create the life cycle of P. karlssoni. The ultrastructure of the agent in lesions of bay scallops is not documented adequately to diagnose affinities to the genus Perkinsus. Furthermore, there was discrepancy as to whether P. karlssoni enlarged in fluid thioglycollate medium, and the shape and structure of the prezoosporangia as well as the movement and life span of the zoospores were not consistent with the genus Perkinsus. P. karlssoni cannot be considered as a species of Perkinsus
Cercariae and diplostomules of Diplostomum spathaceum (Digenea) elicit an immune response in rainbow trout, Salmo gairdneri Richardson
No full textSea lice monitoring on Atlantic salmon farms in New Brunswick, Canada: comparing audit and farm staff counts
No full textStudies on the infectivity of Diplostomum spathaceum in rainbow trout (Oncorhynchus mykiss)
No full textCytotoxic reactions of rainbow trout, Salmo gairdneri Richardson, macrophages for larvae of the eye fluke Diplostomum spathaceum (Digenea)
No full textProtection of rainbow trout, Oncorhynchus mykiss (Richardson), against Diplostomum spathaceum (Digenea): the role of specific antibody and activated macrophages
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