49 research outputs found
Drop-Seq Laboratory Protocol v1
No full text We have continued to optimize this protocol since doing the experiments in the Macosko et al. Cell paper. What we are sharing here is our current, optimized protocol. As a result, this protocol will not precisely match the methods section of the paper, which is a description of the experiments done in the paper. Please feel free to use these optimizations without author consideration - just acknowledge “helpful advice from Evan Macosko, Melissa Goldman and Steve McCarroll”, and please also include the URL and version number of the protocol in your methods section. This protocol also includes hints, suggestions and images that we could not fit into the the methods section of the paper. </p
“Popoffs” Under a Transverse Force Reveal the Number and Location of Active Kinesin Motors During Motility Assays
No full textThermodynamic Venn Diagrams: Sorting Out Forces, Fluxes, and Legendre Transforms
No full textWe show how to use a Venn diagram to illuminate the relations among the different thermodynamic potentials, forces, and fluxes of a simple system. A single diagram shows all of the thermodynamic potentials obtainable by Legendre transformations starting from the internal energy as the fundamental potential. From the diagram, we can also read off the Maxwell relations deduced from each of these potentials. We construct a second Venn diagram that shows the analogous information for the Massieu functions, obtained by Legendre transformations starting from the entropy as the fundamental thermodynamic function
Teaching Entrepreneurship Through Science-Oriented Teams and Projects: Three Case Studies
No full text
