276 research outputs found
Hanover, Washington County
No full textRebekah Carnes, “Hanover, Washington County,” Chapman Center Research Collections, https://ccrsresearchcollections.omeka.net/items/show/75.The author gives a wonderful portrayal of the Hanover, Kansas area. She delves into many aspects surrounding the town and details its settlement using maps, historic documents, and interviews with residents
Editosome Accessory Factors KREPB9 and KREPB10 in Trypanosoma brucei
No full textMultiprotein complexes, called editosomes, catalyze the uridine insertion and deletion RNA editing that forms translatable mitochondrial mRNAs in kinetoplastid parasites. We have identified here two new U1-like zinc finger proteins that associate with editosomes and have shown that they are related to KREPB6, KREPB7, and KREPB8, and thus we have named them Kinetoplastid RNA Editing Proteins, KREPB9 and KREPB10. They are conserved and syntenic in trypanosomatids although KREPB10 is absent in Trypanosoma vivax and both are absent in Leishmania. Tandem affinity purification (TAP)-tagged KREPB9 and KREPB10 incorporate into ∼20S editosomes and/or subcomplexes thereof and preferentially associate with deletion subcomplexes, as do KREPB6, KREPB7, and KREPB8. KREPB10 also associates with editosomes that are isolated via a chimeric endonuclease, KREN1 in KREPB8 RNA interference (RNAi) cells, or MEAT1. The purified complexes have precleaved editing activities and endonuclease cleavage activity that appears to leave a 5' OH on the 3' product. RNAi knockdowns did not affect growth but resulted in relative reductions of both edited and unedited mitochondrial mRNAs. The similarity of KREPB9 and KREPB10 to KREPB6, KREPB7, and KREPB8 suggests they may be accessory factors that affect editing endonuclease activity and as a consequence may affect mitochondrial mRNA stability. KREPB9 and KREPB10, along with KREPB6, KREPB7, and KREPB8, may enable the endonucleases to discriminate among and accurately cleave hundreds of different editing sites and may be involved in the control of differential editing during the life cycle of T. brucei
Chronic musculoskeletal pain rarely presents in a single body site: Results from a UK population study
No full textObjective. To investigate the frequency and health impact of chronic multi-site musculoskeletal pain, in a representative UK sample. Method. Population postal questionnaire survey, using 16 general practices in the southeast of England, nationally representative urban/ rural, ethnic and socioeconomic mix. A random selection of 4049 registered patients, aged 18 or over, were sent a questionnaire. The main outcome measures were chronic pain location, identified using a pain drawing; distress, pain intensity and disability as measured by the GHQ12 and the Chronic Pain Grade. Results. A total of 2445 patients (60%) responded to the survey (44% male, mean age 52 yrs); 45% had chronic musculoskeletal pain. Of those with chronic pain, three quarters had pain in multiple sites (two or more sites). Variables significantly predicting this were: age under 55, [odds ratio (OR) 0.5, 95% confidence interval (CI) 0.4, 0.6]; psychological distress (OR 1.8, CI at 95% 1.4, 2.2) and high pain intensity (OR 5.2, CI at 95% 4.1, 6.7). Only 33% of multi-site pain distributions conformed to the American College of Rheumatology definition of chronic widespread pain. Conclusions. Multi-site chronic pain is more common than single-site chronic pain and is commonly associated with other problems. Indiscriminate targeting of research and care for chronic musculoskeletal pain on single sites may often be inappropriate. © The Author 2007. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved
Chronic Omega-3 Polyunsaturated Fatty Acid Treatment Variably Affects Cellular Repolarization in a Healed Post-MI Arrhythmia Model
No full textIntroduction: Over the last 40 years omega-3 polyunsaturated fatty acids (PUFAs) have been shown to be anti-arrhythmic or pro-arrhythmic depending on the method and duration of administration and model studied. We previously reported that omega-3 PUFAs do not confer anti-arrhythmic properties and are pro-arrhythmic in canine model of sudden cardiac death (SCD). Here, we evaluated the effects of chronic omega-3 PUFA treatment in post-MI animals susceptible (VF+) or resistant (VF-) to ventricular tachyarrhythmias. Methods: Perforated patch clamp techniques were used to measure cardiomyocyte action potential durations (APD) at 50 and 90% repolarization and short term variability of repolarization. The early repolarizing transient outward potassium current I-to was also studied. Results: Omega-3 PUFAs prolonged the action potential in VF- myocytes at both 50 and 90% repolarization. Short term variability of repolarization was increased in both untreated and treated VF- myocytes vs. controls. Ito was unaffected by omega-3 PUFA treatment. Omega-3 PUFA treatment attenuated the action potential prolongation in VF+ myocytes, but did not return repolarization to control values. Conclusions: Omega-3 PUFAs do not confer anti arrhythmic properties in the setting of healed myocardial infarction in a canine model of SCD. In canines previously resistant to ventricular fibrillation (VF-), omega-3 PUFA treatment prolonged the action potential in VF- myocytes, and may contribute to pro-arrhythmic responses.National Institutes of Health [HL089836, HL086700]SCI(E)[email protected]; [email protected]
Historical Dissidence: The Temporalities and Radical Possibilities of American Comics
No full textFormal criticism of comics has often focused on the importance of sequence and the filling of gutters with causative logics. Practitioner-theorists like Will Eisner and Scott McCloud have focused on “sequentiality” and “closure” to conceive of how readers connect the disparate panels of a given comic. More contemporary scholars of the form have followed Eisner and McCloud, foregrounding the causative logics that create narrative progression in the comics form. Yet, these approaches implicitly rely on dominant, western logics of temporality in the construction of narrative in comics. This project considers how comics form actually relies on various temporalities and thus complicates a single, dominant approach to historical consciousness. I argue that comics work as historically dissident cultural productions given the ways comics forms anatomize and even actively question normative western temporality and history. I take a broader approach to form, considering the aesthetic, narrative, and publication elements formally. Such an approach keeps this study from focusing solely on auteur comics or mainstream comics. In fact, I explore these formal elements in comics like Richard McGuire’s Here, Art Spiegelman’s In the Shadow of No Towers, Marvel’s The Uncanny X-Men and Black Panther, Moonshot: The Indigenous Comics Collection, and Red: A Haida Manga. Because comics are ephemeral pulp and because they offer fragmented narratives that are often mutated from accepted forms, they rely on a flexible understanding of how time works, how narratives progress, and what it means to tell a story. Thus, comics forms actually encourage readers to question their own experiences of western, imperial, or heteronormative histories
Supporting Middle Grades Teacher Candidates in Becoming Culturally Competent
No full textThe author describes practices to support middle grades teacher candidates\u27 becoming culturally competent
Construction of an artificial chromosome in Tetrahymena thermophila
No full textStudies of the single cell ciliate Tetrahymena thermophila have provided many insights into telomere structure and function. Telomeres, repetitive sequences at the chromosomal termini, are critical for the stabilization of chromosomes. Telomeres also play important roles in both cancer and aging. Tetrahymena is an excellent system for studying telomeres since each cell possesses more than 40,000 telomeres. One significant drawback of using this organism is that the transformation vectors currently available are relatively rudimentary. The creation of a novel vector, which would exist as an episome, would allow for a more complete investigation of telomere biology. The construction of an artificial chromosome in Tetrahymena thermophila would be a major step in improving complementation analysis in this model organism
Phaedri, Augusti liberti, Fabularum Aesopiarum libri quinque
No full textThis is a lovely book, with, as the seller notes, original marbled-paper endpapers and the original leather binding, and marbled-paper covered boards. I find illustrated Phaedrus editions to be quite rare. None had been done before this one since 1806. This book is in neither Carnes nor Bodemann. However, Carnes has two brief listings of books done in 1837 and 1838, respectively, and with the same exact title and page count as this volume. The word schematibus in the title of both appears in only these two titles in the whole of Carnes' catalogue. Here are Carnes' two references: Phaedri, Augusti liberti, Fabularum Aesopiarum libri quinque / quibus accedunt fabulae novae ex codice Perottino. Ed. nova ex optimis exemplaribus emendata et schematibus illustrata. Paris: Henriot, 1837, 172 pp. UBN. Nijmegen. And Phaedri Fabularum Aesopiarum libri quinque, quibus accedunt fabulae novae ex codice Perottino. Editio nova, ex optimis exemplaribus emendata et schematibus illustrata. Paris: R. Bregeaut, 1838. 172 pp. LC, NYPL. Bodemann does have Delloye publishing a Florian in 1838. The artist there was Victor Adam. Might he have been the artist here too? Jules David also did a LaFontaine in 1837/38, as did of course Grandville. The illustrations -- and their printing here -- are of high quality. There are also abundant printer's devices. A strange thing happens after the eighteenth fable of Book I: the text changes from movable print to script. That pattern perdures through Fable XXVI of the first book. In that section, the printer's designs are also replaced by hand-drawn symbols. The paper is also thinner. On 36, a strong design sweeps outside the margins. Who were the illustrators here? One of the fine partial-page designs is signed T. P. Molet (52), who is not in Bodemann. Another is signed H. Faxardo (64), also not in Bodemann. Still another is signed Lesestre, who is listed in Bodemann for engraving a La Fontaine in 1851. He may have the best fable illustration picturing the owl and cicada on 86. Verdeil on 73 is listed three times in Bodemann between 1837 and 1852. Altogether, this is a fascinating and unusual book! Is it an insult to a book to say that its best feature may be its printer's devices? There is an AI at the back.This is a hardbound book (hard cover)Language note: LatinPhaedru
Software for analyzing data contained in output films created by the SPATL and MLTCRP routines of the accuracy assessment software system
No full textThere are no author-identified significant results in this report
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