239 research outputs found
Insights into the molecular etiology of exercise-induced inflammation: opportunities for optimizing performance
No full textIoannis G Fatouros, Athanasios Z Jamurtas School of Physical Education and Sport Sciences, University of Thessaly, Karies, Trikala, Greece Abstract: The study of exercise-induced muscle damage (EIMD) is of paramount importance not only because it affects athletic performance but also because it is an excellent model to study the mechanisms governing muscle cachexia under various clinical conditions. Although, a large number of studies have investigated EIMD and its associated inflammatory response, several aspects of skeletal muscles responses remain unclear. In the first section of this article, the mechanisms of EIMD are reviewed in an attempt to follow the events that result in functional and structural alterations of skeletal muscle. In the second section, the inflammatory response associated with EIMD is presented with emphasis in leukocyte accumulation through mechanisms that are largely coordinated by pro- and anti-inflammatory cytokines released either by injured muscle itself or other cells. The practical applications of EIMD and the subsequent inflammatory response are discussed with respect to athletic performance. Specifically, the mechanisms leading to performance deterioration and development of muscle soreness are discussed. Emphasis is given to the factors affecting individual responses to EIMD and the resulting interindividual variability to this phenomenon. Keywords: muscle damage, exercise, aseptic inflammation, recovery, immune system, redox statu
Exercise-Induced Muscle Damage and Oxidative Stress
No full textExercise-induced muscle damage (EIMD) is associated with muscle soreness or discomfort and amarked decline of muscle strength during the first 12–72 hours post-exercise [1].[...
The dancer as a performing athlete: physiological considerations.
No full textThe physical demands placed on dancers from current choreography and performance schedules make their physiology and fitness just as important as skill development. However, even at the height of their professional careers, dancers' aerobic power, muscular strength, muscular balance, bone and joint integrity are the 'Achilles heels' of the dance-only selection and training system. This partly reflects the unfounded view, shared by sections of the dance world, that any exercise training that is not directly related to dance would diminish dancers' aesthetic appearances. Given that performing dance itself elicits only limited stimuli for positive fitness adaptations, it is not surprising that professional dancers often demonstrate values similar to those obtained from healthy sedentary individuals of comparable age in key fitness-related parameters. In contrast, recent data on male and female dancers revealed that supplementary exercise training can lead to improvements of such fitness parameters and reduce incidents of dance injuries, without interfering with key artistic and aesthetic requirements. It seems, however, that strict selection and training regimens have succeeded in transforming dance to an activity practised by individuals who have selectively developed different flexibility characteristics compared with athletes. Bodyweight targets are normally met by low energy intakes, with female dance students and professional ballerinas reported to consume below 70% and 80% of the recommended daily allowance of energy intake, respectively, while the female athlete 'triad' of disordered eating, amenorrhoea and osteoporosis is now well recognised and is seen just as commonly in dancers.An awareness of these factors will assist dancers and their teachers to improve training techniques, to employ effective injury prevention strategies and to determine better physical conditioning. However, any change in the traditional training regimes must be approached cautiously to ensure that the aesthetic content of the dance is not affected by new training techniques. Since physiological aspects of performing dance have been viewed primarily in the context of ballet, further scientific research on all forms of dance is required
Association of fat mass profile with natriuretic peptide receptor alpha in subcutaneous adipose tissue of medication-free healthy men: A cross-sectional study [version 2; referees: 2 approved]
No full textBackground: Atrial natriuretic peptide increases lipolysis in human adipocytes by binding to natriuretic peptide receptor-A (NPRA). The aim of the current study was to examine the associations of NPRA mRNA of subcutaneous adipose tissue with fat mass, fat-free mass, body mass index (BMI) and arterial blood pressure in medication-free healthy men. Method: Thirty-two volunteers [age (years): 36.06±7.36, BMI: 27.60±4.63 (kg/m2)] underwent assessments of body height/weight, % fat mass, fat-free mass (kg), blood pressure, and a subcutaneous adipose tissue biopsy via a surgical technique. Results: We found that NPRA mRNA was negatively associated with % fat mass (r=-0.40, R2=0.16, p=0.03) and BMI (r=-0.45, R2=0.20, p=0.01). Cohen’s f2 effect size analyses showed a small effect size between NPRA mRNA and BMI (f2=0.25). One-way analysis of variance with Bonferroni post-hoc tests showed a tendency for mean differences of NPRA mRNA across BMI categories (p=0.06). This was confirmed by Cohen’s d effect size analyses revealing a large effect size of NPRA mRNA between obese individuals (BMI≥30 kg/m2) and either normal weight (BMI=19-25 kg/m2; d=0.94) or overweight (BMI=25-30 kg/m2; d=1.12) individuals. Conclusions: NPRA mRNA is negatively associated with % fat mass and BMI in medication-free healthy men, suggesting a possible role of NPRA in the control of fat mass accumulation
Unilateral Plyometric Training is Superior to Volume-Matched Bilateral Training for Improving Strength, Speed and Power of Lower Limbs in Preadolescent Soccer Athletes
No full textThis study compared the effects of unilateral and bilateral plyometric training on strength, sprint performance and lower limb power. Sixty-eight preadolescent soccer athletes were randomly assigned to a unilateral plyometric training group (n=23), a bilateral plyometric training group (n=23) and a control group (n=22). Both plyometric training groups trained with equal volumes of unilateral or bilateral exercises for 15 minutes in each session, at which time the control group performed soccer-specific drills. Plyometric exercises were executed twice weekly for 10 weeks during the competitive season. The following tests were performed before and after the intervention: single-leg and double-leg countermovement jump, squat jump, horizontal jumps in different directions, maximal isometric strength of quadriceps and hamstrings, sprint performance, agility and balance. Unilateral plyometric training resulted in greater improvements compared to the control group in the following variables: hamstrings strength (ES: 0.91, p=0.037), 5m sprint time (ES: 0.93, p=0.004), single-leg countermovement jump (ES: 0.90, p=0.006), single- and double-leg squat jump (ES: 0.87, p=0.030 and ES: 0.73, p=0.067, respectively) and single-leg hop performance (ES: 1.01, p=0.004). The only tests where there was an improvement of BPT compared with the CG were the single-leg and double leg SJ (ES: 0.76, p=0.026; ES: 0.70, p=0.050). Quadriceps strength, side hop test, double-leg horizontal jump test, flamingo balance test and modified agility T-test were equally improved in all three groups (p<0.001). In conclusion, unilateral lower-limb plyometric training is more effective in increasing muscle strength and power in preadolescent soccer players when compared to bilateral training or soccer training alone. © 2020 Vasileios Drouzas, Christos Katsikas, Andreas Zafeiridis, Athanasios Z. Jamurtas, Gregory C. Bogdanis, published by Sciendo 2020
Unilateral Plyometric Training is Superior to Volume-Matched Bilateral Training for Improving Strength, Speed and Power of Lower Limbs in Preadolescent Soccer Athletes
No full textThis study compared the effects of unilateral and bilateral plyometric training on strength, sprint performance and lower limb power. Sixty-eight preadolescent soccer athletes were randomly assigned to a unilateral plyometric training group (n=23), a bilateral plyometric training group (n=23) and a control group (n=22). Both plyometric training groups trained with equal volumes of unilateral or bilateral exercises for 15 minutes in each session, at which time the control group performed soccer-specific drills. Plyometric exercises were executed twice weekly for 10 weeks during the competitive season. The following tests were performed before and after the intervention: single-leg and double-leg countermovement jump, squat jump, horizontal jumps in different directions, maximal isometric strength of quadriceps and hamstrings, sprint performance, agility and balance. Unilateral plyometric training resulted in greater improvements compared to the control group in the following variables: hamstrings strength (ES: 0.91, p=0.037), 5m sprint time (ES: 0.93, p=0.004), single-leg countermovement jump (ES: 0.90, p=0.006), single- and double-leg squat jump (ES: 0.87, p=0.030 and ES: 0.73, p=0.067, respectively) and single-leg hop performance (ES: 1.01, p=0.004). The only tests where there was an improvement of BPT compared with the CG were the single-leg and double leg SJ (ES: 0.76, p=0.026; ES: 0.70, p=0.050). Quadriceps strength, side hop test, double-leg horizontal jump test, flamingo balance test and modified agility T-test were equally improved in all three groups (p<0.001). In conclusion, unilateral lower-limb plyometric training is more effective in increasing muscle strength and power in preadolescent soccer players when compared to bilateral training or soccer training alone. © 2020 Vasileios Drouzas, Christos Katsikas, Andreas Zafeiridis, Athanasios Z. Jamurtas, Gregory C. Bogdanis, published by Sciendo 2020
Low-Grade Systemic Inflammation Interferes with Anabolic and Catabolic Characteristics of the Aged Human Skeletal Muscle.
No full textAging is associated with the development of chronic low-grade systemic inflammation (LGSI) characterized by increased circulating levels of proinflammatory cytokines and acute phase proteins such as C-reactive protein (CRP). Collective evidence suggests that elevated levels of inflammatory mediators such as CRP, interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α) are correlated with deteriorated skeletal muscle mass and function, though the molecular footprint of this observation in the aged human skeletal muscle remains obscure. Based on animal models showing impaired protein synthesis and enhanced degradation in response to LGSI, we compared here the response of proteolysis- and protein synthesis-related signaling proteins as well as the satellite cell and amino acid transporter protein content between healthy older adults with increased versus physiological blood hs-CRP levels in the fasted (basal) state and after an anabolic stimulus comprised of acute resistance exercise (RE) and protein feeding. Our main findings indicate that older adults with increased hs-CRP levels demonstrate (i) increased proteasome activity, accompanied by increased protein carbonylation and IKKα/β phosphorylation; (ii) reduced Pax7+ satellite cells; (iii) increased insulin resistance, at the basal state; and (iv) impaired S6 ribosomal protein phosphorylation accompanied by hyperinsulinemia following an acute RE bout combined with protein ingestion. Collectively, these data provide support to the concept that age-related chronic LGSI may upregulate proteasome activity via induction of the NF-κB signaling and protein oxidation and impair the insulin-dependent anabolic potential of human skeletal muscle
The Yo-Yo Intermittent Endurance Level 2 Test: Reliability of Performance Scores, Physiological Responses and Overload Characteristics in Competitive Soccer, Basketball and Volleyball Players
No full textThe purpose of this study was to evaluate the reliability and reproducibility of the physiological and overload features of the Yo-Yo intermittent endurance test level 2 (Yo-Yo IE2) in competitive male soccer (n = 20), basketball (n = 11), and volleyball players (n = 10). The participants completed Yo-Yo IE2 tests on three separate occasions with assessment of performance, heart rate, running speed, accelerations, decelerations and body load using GPS instrumentation. The intra-class correlation coefficient index, confidence intervals and coefficients of variation were calculated to assess the reliability of the test. Intra-class correlation coefficients for test-retest trials in the total sample ranged from large to nearly perfect (total distance: 0.896; mean speed: 0.535; maximum speed: 0.715; mean HR: 0.876; maximum HR: 0.866; body load: 0.865). The coefficients of variation for distance, mean speed, HR response, as well as acceleration and deceleration scores for test-retest trials ranged from 1.2 to 12.5% with no differences observed among particular sport disciplines. The CV for shuttles performed ranged from 4.4 to 5.5% in all sports. Similar results were obtained for the three different categories of players tested. These results suggest that the Yo-Yo IE2 test appears to be a reliable alternative for evaluating the ability to perform intermittent high-intensity running in different outdoor and indoor team sports. Players may need one or two familiarization tests to ensure valid assessment of intermittent endurance capacity. It appears that the Yo-Yo IE2 test incorporates accelerations and decelerations in a consistent and reproducible fashion. © 2019 Konstantinos Papanikolaou, Athanasios Chatzinikolaou, Theodoros Pontidis, Alexandra Avloniti, Chariklia K. Deli, Diamanda Leontsini, Dimitrios Draganidis, Panagiotis D. Tsimeas, Lefteris Rafailakis, Athanasios Z. Jamurtas, Peter Krustrup, Magni Mohr, Ioannis G. Fatouros published by Sciendo 2019
Browning formation markers of subcutaneous adipose tissue in relation to resting energy expenditure, physical activity and diet in humans
No full textBackground Regular exercise and diet may contribute to white adipose tissue (WAT) conversion into a brown adipose-like phenotype that may increase resting energy expenditure (REE), leading to weight loss. We examined the relationship between REE, physical activity (PA) participation and diet with browning formation markers of subcutaneous WAT in healthy men. Materials and methods We assessed REE, diet and body composition of 32 healthy men [age (years): 36.06 ± 7.36, body mass index (BMI): 27.06 ± 4.62 (kg/m2)]. Participants also underwent measurements of PA [metabolic equivalent (MET)-min/week] using the International Physical Activity Questionnaire (IPAQ), while they undertook a subcutaneous fat biopsy from the abdominal region to assess the mRNA expressions of uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ). Results We found no associations between the UCP1, PGC-1α, PPARα and PPARγ mRNAs with REE, PA levels and diet (p > 0.05). However, the PGC-1α, PPARα and PPARγ mRNAs were more expressed in individuals displaying moderate rather than low PA levels (p < 0.05). Furthermore, PGC-1α, PPARα and PPARγ mRNAs were negatively correlated with fat mass percentage (p < 0.05). PGC-1α and PPARα mRNAs were also negatively correlated with BMI, while PGC-1α mRNA was inversely associated with waist-to-hip ratio (p < 0.05). Conclusion REE, PA levels and diet are not associated with browning formation indices of subcutaneous adipose tissue in healthy adult men
Acute and short-term effects of secondhand smoke on lung function and cytokine production.
No full textRATIONALE: The acute effect of secondhand smoke (SHS) on lung function and the duration of system disruption remain unknown. OBJECTIVES: To assess the SHS effects and their duration on lung function and inflammatory markers. METHODS: In a randomized single-blind crossover experiment data were obtained from 16 (8 women) nonsmoking adults at baseline and at 0, 1, and 3 hours after a 1-hour SHS exposure set at bar/restaurant SHS levels. MEASUREMENTS AND MAIN RESULTS: Serum and urine cotinine, lung function, and cytokines IL-4, IL-5, IL-6, tumor necrosis factor (TNF)-alpha, and IFN-gamma. At 0 hours most lung function parameters were significantly reduced (indicative: FEV(1), 4.3 +/- 0.4 vs. 3.8 +/- 0.3 L; FEV(1)/FVC, 0.9 +/- 0.1 vs. 0.8 +/- 0.1; P < 0.05) but at 3 hours they were at baseline levels. In contrast, cotinine (serum, 8.9 +/- 3.2 vs. 35.5 +/- 10.2 ng x ml(-1)), IL-4 (41.3 +/- 5.8 vs. 44.2 +/- 4.5 pg x ml(-1)), IL-5 (36.1 +/- 3.2 vs. 60.1 +/- 7.0 pg x ml(-1)), IL-6 (2.5 +/- 0.3 vs. 7.6 +/- 1.4 pg x ml(-1)) and IFN-gamma (0.3 +/- 0.2 vs. 0.6 +/- 0.2 IU x ml(-1)) at 3 hours were higher than at baseline (P < 0.05). IL-4 and TNF-alpha increased only in men, whereas IL-5, IL-6, and IFN-gamma were different between sexes after exposure (P < 0.05). Regression analyses revealed inverse associations of FEV(1) and FEV(1)/FVC ratio with IL-5 (P < 0.05) in men and with IL-5 (P = 0.01), IL-6 (P < 0.001), IFN-gamma (P = 0.034) and serum cotinine (P < 0.001) in women. CONCLUSIONS: We conclude that 1 hour of SHS exposure at bar/restaurant levels is accompanied by significant decrements on lung function and marked increases in inflammatory cytokines, particularly in men. More importantly, whereas most smoke-induced effects on lung function appear to recede within 60 minutes, inflammatory cytokines remain elevated for at least 3 hours after exposure to SHS
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