430 research outputs found
Peripheral Blood RNA Sequencing Unravels a Differential Signature of Coding and Noncoding Genes by Types of Kidney Allograft Rejection
No full textIntroduction: Peripheral blood (PB) molecular patterns characterizing the different effector immune pathways driving distinct kidney rejection types remain to be fully elucidated. We hypothesized that transcriptome analysis using RNA sequencing (RNAseq) in samples of kidney transplant patients would enable the identification of unique protein-coding and noncoding genes that may be able to segregate different rejection phenotypes. Methods: We evaluated 37 biopsy-paired PB samples from the discovery cohort, with stable (STA), antibody-mediated rejection (AMR), and T cell-mediated rejection (TCMR) by RNAseq. Advanced machine learning tools were used to perform 3-way differential gene expression analysis to identify gene signatures associated with rejection. We then performed functional in silico analysis and validation by Fluidigm (San Francisco, CA) in 62 samples from 2 independent kidney transplant cohorts. Results: We found 102 genes (63 coding genes and 39 noncoding genes) associated with AMR (54 upregulated), TCMR (23 upregulated), and STA (25 upregulated) perfectly clustered with each rejection phenotype and highly correlated with main histologic lesions (r = 0.91). For the genes associated with AMR, we found enrichment in regulation of endoplasmic reticulum stress, adaptive immunity, and Ig class-switching. In the validation, we found that the SIGLEC17P pseudogene and 9 SIGLEC17P-related coding genes were highly expressed among AMR but not in TCMR and STA samples. Conclusions: This analysis identifies a critical gene signature in PB in kidney transplant patients undergoing AMR, sufficient to differentiate them from patients with TCMR and immunologically quiescent kidney allografts. Our findings provide the basis for new studies dissecting the role of noncoding genes in the pathophysiology of kidney allograft rejection and their potential value as noninvasive biomarkers of the rejection process
Impacts of Ta Buffer Layer and Cu-Ge-Te Composition on the Reliability of GeSe-Based CBRAM
No full textsponsorship: This work was supported by imec's Core Partner Industrial Affiliation Program on Emerging Memories. The review of this article was arranged by Editor P. Du. (Corresponding author: Janaki Radhakrishnan.) (imec's Core Partner Industrial Affiliation Program on Emerging Memories)status: Publishe
Efficacy of sugarcane bagasse to produce bacterial biofilm in water for fish culture
No full textThe present study has been conducted to understand the efficacy of sugarcane bagasse to produce bacterial biofilm in water. The study period was 90 days. Total Plate count (TPC) in water and substrate was estimated on nutrient agar at room temperature by spread plate method. The TPC in water was the highest (2.10 x 104 ml–1) after 45 days. The average TPC of bacteria on bagasse varied from 140.0 (15d) to 30.25 (90d) x104·g–1. The present study demonstrates that the sugarcane bagasse can produce more bacteria in water thereby the fish can effectively utilize biofilm grown on sugarcane bagasse and provision of a substrate reduces the need for artificial feed.---------------------------------------------------------------------------------------------------------Efficacy of sugarcane bagasse to produce bacterial biofilm in water for fish culture M.V. Radhakrishnan and E.SugumaranDepartment of Zoology, Annamalai University, Annamalainagar – 608 002, Tamilnadu, India *Corresponding Author, Email: [email protected] Cite This Article As: M.V. Radhakrishnan, E. Sugumaran. 2010. Efficacy of sugarcane bagasse to produce bacterial biofilm in water for fish culture. J. Ecobiotechnol. 2(2):41-44
Baclofen-induced neurotoxicity in a patient with end-stage renal disease
No full textBaclofen, predominantly excreted by the kidneys is accumulated in patients with renal insufficiency leading to the central nervous system toxicity. Here the author reports a patient with end-stage renal disease on maintenance hemodialysis (HD) who developed drowsiness and became unresponsive within a day after taking single 10 mg dose of baclofen. Patient improved completely after two sessions of HD
Faculty Opinions recommendation of Transcriptomic analysis uncovers novel synergistic mechanisms in combination therapy for lupus nephritis.
No full textThe burden of prescription coverage of kidney failure patients in the United States: is Medicare Part D the answer?
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