322 research outputs found
The complex route to MHC class I-peptide complexes
No full textThe loading of peptides into the groove of MHC class I molecules prior to antigen presentation is a complex process. In this issue of Cell, Park et al. (2006) show that peptide loading gets a helping hand from a resident ER enzyme called protein disulfide isomerase, a chaperone that has oxidoreductase activit
Response: commentary: an in silico- in vitro pipeline identifying an HLA-A*02:01+KRAS G12V+spliced epitope candidate for a broad tumor-immune response in cancer patients
No full textChemical Immunolog
Ajmalicineproductie door Catharantus roseus
No full textDocument(en) uit de collectie Chemische Procestechnologie.DelftChemTechApplied Science
The Immunoproteasome Cleans up after Inflammation
No full textImmune cells and cells activated by the inflammatory cytokine interferon express variant proteasomes called immunoproteasomes that are characterized by unique catalytic subunits. Seifert et al. (2010) now show in mouse models of inflammatory disease that immunoproteasomes help prevent the accumulation of harmful protein aggregates
Intracellular transport and peptide loading of MHC class II molecules: regulation by chaperones and motors
No full text
Soft agar colony formation assay to quantify mouse embryonic fibroblast transformation after Salmonella infection
No full textSummary: Links between bacterial infections and cancer are actively investigated. Cost-effective assays to quantify bacterial oncogenic potential can shed new light on these links. Here, we present a soft agar colony formation assay to quantify mouse embryonic fibroblast transformation after Salmonella Typhimurium infection. We describe how to infect and seed cells in soft agar for anchorage-independent growth, a hallmark of cell transformation. We further detail automated cell colony enumeration. This protocol is adaptable to other bacteria or host cells.For complete details on the use and execution of this protocol, please refer to Van Elsland et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics
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