27 research outputs found
« Vos pensées ne sont pas mes pensées et mes chemins ne sont pas vos chemins » (Isaïe 55.8) : une réflexion sur le thème de l’ordre dans les Suspiria de Profundis (1845) de Thomas De Quincey
No full textThis article looks at the theme of order in Thomas De Quincey’s Suspiria de Profundis (1845). Although chaos is easily noticeable in De Quincey’s digressional style, order is also a recurring motif in the narrative of this mature work. Indeed, for the Protestant and Romantic author plagued by suffering, chaos and order are the starting points for broader reflections. De Quincey discovers that chaos in the outer and inner worlds is but apparent, and that deciphering these worlds enables him to see a unifying or organizing force beneath contraries and paradoxes. As a consequence, in his hermeneutics based on the scandal of suffering, De Quincey introduces the notion of obliqueness operating through the masks behind which God acts, thus retracing the very paths taken by God and disclosing his own theology
Comparison and practical review of segmentation approaches for label-free microscopy
No full text<p>This dataset contains microscopic images of PNT1A cell line captured by multiple microcopic without use of any labeling and a manually annotated ground truth for subsequent use in segmentation algorithms. Dataset also includes images reconstructed according to the methods described below in order to ease further segmentation. </p>
<p>See Vicar et al. Cell segmentation methods for label-free contrast microscopy: review and comprehensive comparison. BMC Bioinformatics (2019) 20:360. DOI <a href="https://doi.org/10.1186/s12859-019-2880-8">10.1186/s12859-019-2880-8</a></p>
<p>Code using this dataset is available at <a href="https://github.com/tomasvicar/Cell-segmentation-methods-comparison">https://github.com/tomasvicar/Cell-segmentation-methods-comparison</a></p>
<p><strong>Materials and methods </strong></p>
<p>Cells were cultured in RPMI-1640 medium supplemented with antibiotics (penicillin 100 U/ml and streptomycin 0.1 mg/ml) with 10% fetal bovine serum. Prior microscopy acquisition, cells were maintained at 37 cenigrade in a humidified incubator with 5% CO2. Intentionally, high passage number of cells was used (>30) in order to describe distinct morphological heterogeneity of cells (rounded and spindle-shaped, relatively small to large polyploid cells). For acquisition purposes, cells were cultivated in Flow chambers µ-Slide I Luer Family (Ibidi, Martinsried, Germany).</p>
<p>Quantitative phase imaging (QPI) microscopy was performed on Tescan Q-PHASE (Tescan, Brno, Czech republic), with objective Nikon CFI Plan Fluor 10x/0.30 captured by Ximea MR4021MC (Ximea, Münster, Germany). Imaging is based on the original concept of coherence-controlled holographic microscope \cite{Kolman:10,Slaby:13}, images are shown in grayscale with units of pg/µm2.</p>
<p>DIC microscopy was performed on microscope Nikon A1R (Nikon, Tokyo, Japan), with objective Nikon CFI Plan Apo VC 20x/0.75 captured by CCD camera Jenoptik ProgRes MF (Jenoptik, Jena, Germany). </p>
<p>HMC microscopy was performed on microscope Olympus IX71 (Olympus, Tokyo, Japan), with objective Olympus CplanFL N 10x/0.3 RC1 captured by CCD camera Hamamatsu Photonics ORCA-R2 (Hamamatsu Photonics K.K., Hamamatsu, Japan).</p>
<p>PC microscopy was performed on a Nikon Eclipse TS100-F microscope, with a Nikon CFI Achro ADL 10x/0.25 objective captured by CCD camera Jenoptik ProgRes MF.</p>
<p><strong>Folder structure and file and filename description</strong><br>
<br>
<em>folder "source data+groundtruth"</em><br>
- includes raw microscopic data <br>
(uncompressed 16-bit for DIC, HMC and PC, 32-bit for QPI)<br>
- includes manualy annotated groundtruth (zip file - imageJ ROI file, 1bit png mask)</p>
<p>e.g. <br>
DIC_01_raw.tif<br>
DIC_01_groundtruth_imagejROI.zip<br>
DIC_01_groundtruth_mask.png</p>
<p><br>
<em>folder "reconstructions"</em></p>
<p>includes reconstructed images using reconstructions with highest dice coefficient achieved. </p>
<p>for DIC and HMC: rDIC-Koos, rDIC-Yin, and rWeka<br>
for PC: rPC-Top-Hat, rDIC-Yin, and rWeka<br>
for QPI: rWeka</p>
<p>note that for rWeka images numbered 01 for DIC, HMC and PC and 01-03 for QPI were used for learning.</p>
<p><strong>Abbreviations</strong><br>
DIC, differential image contrast<br>
HMC, Hoffman modulation contrast<br>
PC, phase contrast<br>
QPI, quantitative phase imaging<br>
rDIC-Koos, DIC/HMC image reconstruction according to Koos et al, Sci Rep. 2016;6:30420<br>
rDIC-Yin, DIC/HMC image reconstruction according to Yin et al, Inf Process Med Imaging. 2011;22:384-97.<br>
rPC-Yin, PC image reconstruction according to Yin et al, Med Im Anal. 2012; 16(5):1047<br>
rPC-Top-Hat, Top-Hat filter according to Dewan et al, IEEE Transactions on Biomedical Circuits and<br>
Systems.2014;8(5):716-728<br>
rWeka, probability map using Trainable Weka segmentation according to Arganda-Carreras et al. Bioinformatics. 2017</p>This work was supported by the Czech Science Foundation GACR 18-24089
Aesthetic "Existenzerhellung" and subversive texture
No full textDas 2019 mit dem Nobelpreis für Literatur ausgezeichnete Werk Peter Handkes gehört zu den bedeutendsten Beiträgen der deutschen Gegenwartsliteratur. Dem relativ gut erforschten Werk der frühen und mittleren Schreibperiode Handkes steht ein bislang kaum untersuchtes Spätwerk gegenüber, in dem der teilweise umstrittene politische Autor zeitkritische Gegenwartsdiagnosen mit subtilen Auslo-tungen literarästhetischer Schreibstrategien miteinander verknüpft.
Ausgehend vom existenzialistisch geprägten Grundansatz des Handkeschen Schreibens, wie er sich vor allem in Handkes frühem Werk abbildet, entwickelt der Forschungsbeitrag einen systematischen Doppelzugriff auf Handkes Oeuvre bis hin zu den jüngsten vorgelegten Prosabeiträgen. Während der Deutungsansatz im Begriff der auf Karl Jaspers‘ Philosophie zurückgehenden „Existenzerhellung“ auf Handkes Kunst einer existenziell und phänomenologisch ausgerichteten Kunst des ästhetischen Erfahrung-Machens verweist, untersucht der Autor am Leitbegriff der subversiven Textur Handkes ästhetisch-literarische Diagnose von Phänome-nen gesellschaftlicher Entfremdung. In der Zusammenschau beider Unter-suchungslinien wird deutlich, dass Handkes Oeuvre auch dort, wo es nicht vor-dergründig um existenzielle Kategorien wie Tod oder Angst kreist, Grundsituatio-nen der Befindlichkeit des modernen Menschen reflektiert und ästhetisch eindring-lich problematisiert.
Neben der Ausleuchtung der in Handkes Texten zahlreich angereicherten intertextuellen Verbindungslinien wartet die Untersuchung mit theoretischen Annäherungen und Neuverständigungen zu Handkes Spätwerk auf. Hierzu zählen die interdisziplinären Berührungspunkte zur philosophischen Theorie der ästhetischen Erfahrung und Phänomenologie ebenso wie die Einbezie-hung von Wolfram Hogrebes Ansatz eines ästhetischen „Zwischenreichs“. Im methodischen Gesamtertrag der Arbeit zeichnet sich die unverbrüchliche Zusammengehörigkeit und Interdependenz von Handkes ästhetischen und philosophischen Deutungsinteressen einer Wirklichkeit der Literatur, der Sprache und der gelebten Erfahrung ab.Peter Handke's work, which was awarded the Nobel Prize for Literature in 2019, is one of the most important contributions to contemporary German literature. The relatively well-researched work of Handke's early and middle writing period contrasts with a late work that has hardly been researched to date, in which the sometimes controversial political author combines con-temporary diagnoses with subtle explorations of literary-aesthetic writing strategies.
Starting from the basic existentialist approach of Handke's writing, as re-flected above all in his early work, the research article develops a system-atic double approach to Handke's oeuvre up to the most recent prose con-tributions. While the interpretative approach in the concept of "Existen-zerhellung", which goes back to Karl Jaspers' philosophy, refers to Hand-ke's art of an existentially and phenomenologically orientated art of aesthet-ic experience-making, the author uses the guiding concept of “subversive texture” to examine Handke's aesthetic-literary diagnosis of phenomena of social alienation. In the synopsis of both lines of investigation, it becomes clear that Handke's oeuvre, even where it does not primarily revolve around existential categories such as death or fear, reflects fundamental situations of the state of modern man and problematises them in an aesthetically penetrating way.
In addition to illuminating the numerous intertextual connections enriched in Handke's texts, the study offers theoretical approaches and new under-standings of Handke's late work. These include interdisciplinary points of contact with the philosophical theory of aesthetic experience and phenome-nology as well as the inclusion of Wolfram Hogrebe's approach of an aes-thetic "intermediate realm". The overall methodological outcome of the work reveals the unbreakable cohesion and interdependence of Handke's aes-thetic and philosophical interests in interpreting a reality of literature, lan-guage and lived experience
Simple solvothermal approach to highly nanostructured hematite thin films
No full textIn this work, we present a solvothermal method for the synthesis of hematite thin films on fluorine-doped tin
oxide substrates. This simple method uses a precursor solution of iron(III) 2,4-pentanedionate dissolved in ethanol with a microliter-scale amount of water and yields hematite ~ 500-nm thick films after annealing. The synthesised films were characterised using an array of methods, including scanning electron microscopy, energy-dispersive X-ray spectroscopy, diffuse reflectance, and powder x-ray diffraction. Incorporating water into the precursor solution provides nucleation sites for the reaction and results show that by altering the amount of water used in the synthesis, it is possible to generate nanocrystalline films of different morphologies, nanocrystal size distributions, and surface areas. This synthetic procedure therefore provides control over the films’ physical and electrochemical characteristics. Doping of hematite thin films is also possible using this synthesis, as exemplified by doping with tin by adding tin(II) 2,4-pentanedionate to the precursor solution. To demonstrate utility, we build prototype photoelectrochemical cells using the synthesized hemtatite as the photoanode.The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
Abstract 1492: Prediction of cancer progression in Barrett's esophagus patients using miRNA profiling
No full textAbstract 4006: MiRNA profiling in esophageal precancerous for malignancy progression risk assessment
No full textA peer mediation program piloted in the fourth grade at Black Earth Elementary School
No full textPlan BDespite concerns about increasing violence by educational officials, parents, and community members, most school programs do not provide students specific educational experiences that facilitate adequate personal and social development. Some schools, nationally, have addressed this need by offering educational programs teaching conflict resolution skills through peer mediation. The purpose of this study was to determine whether a peer mediation program piloted in a fourth grade population would encourage students to use conflict resolution skills to resolve disputes peacefully. The model used is called the Peers in Education Addressing Conflict Effectively (PEACE) Program, whose goal is to teach appropriate conflict resolution skills, and decrease hostility in the school environment.
Fifteen mediators, selected by their peers, were taught the PEACE program skills of conflict resolution. These skills include communication, reflective listening, identifying feelings and problem solving. The mediators were trained by the author and another staff member who had been trained in the PEACE Program.
The time frame of the study was from January 1999 to January 2000. Information was gathered through questionnaires given to peer mediators, a questionnaire answered by the fourth grade population and fourth grade teachers, anecdotal records and observations from participating teachers and the school principal. Results of the study did not show a reduction in antisocial behavior or reduced violence in the school. The findings did suggest that the mediators learned the conflict resolution skills, but because of the time schedule, opportunities to practice and use them were limited. Students and teachers did demonstrate positive interest in the program; students wanted to become trained mediators and teachers and administrators supported the concept of peaceful problem-solving. Data collected from the study will be used to evaluate the effectiveness of the program, identifying strengths and weaknesses for future program implementations
Diagnostic and prognostic potential of miR-21, miR-29c, miR-148 and miR-203 in adenocarcinoma and squamous cell carcinoma of esophagus
No full textDo regional systems of innovation matter?
No full textThe paper deals with the significant impact of location on innovation activity that has been found in many empirical studies. Main elements of such an explanation are the specific problems of a division of innovative labor. Based on an outline of these issues the concept of a regional innovation system is presented in some detail and an overview of results from recent research on regional innovation systems is given. The paper then discusses basic policy options and names a number of important questions for further research. -- Der Beitrag behandelt den Einfluss regionaler Gegebenheiten auf Innovationsakti-vitäten, wie er in diversen empirischen Studien nachgewiesen wurde. Ein wesentliches Element bei der Erklärung der Bedeutung von Standortgegebenheiten sind die besonde-ren Probleme der Arbeitsteiligkeit von Innovationsprozessen. Ausgehend von einem Abriss dieser Besonderheiten wird das Konzept des regionalen Innovationssystems er-läutert und ein Überblick über neuere Forschungsergebnisse gegeben. Schließlich wer-den grundlegende innovationspolitische Strategien diskutiert und wesentliche For-schungsfragen benannt.Innovation,regional innovation systems,division of innovative labor,clusters,technology policy,Innovation,regional innovation systems,division of innovative labor,clusters,technology policy
A plan for promoting the resiliency of suicidal adolescents
No full textPlan BThis review of literature and projected study will explore the need for an investigation into the understanding of, the sources of support for, and resiliency for adolescents who have contemplated, but not attempted suicide. It will also outline the process used to obtain and interview youth for a pilot study. It is hypothesized that students draw their resiliency from many sources that are connected to relationships, such as support and open communication either with parents, siblings, grandparents, teachers, pastors, mentors, or friends. It is also hypothesized that students feel an internal sense of being needed or valued by someone or by themselves in a way which gives them the strength to carry on for another day. The process outlined here will provide for a qualitative study that will clarify both the sources and types of resilience to be identified for the testing of these two hypotheses.
A results and discussion section will detail difficulties the author encountered in carrying a full-length pilot study of resilience in suicidal prone adolescents. Because of current conditions in U.S. schools, prospective researchers may expect school personnel to consider carefully the risks they perceive as attendant upon a study of this kind. The methodology section of the paper has also been adapted to reflect a proposed study rather than a retrospective research study
