51 research outputs found
The role of pH dynamics and the Na+/H+ antiporter in the etiopathogenesis and treatment of cancer. Two faces of the same coin-one single nature
No full textRegulatory Considerations in Application of Encapsulated Cell Therapies
No full textThe encapsulation of tissue in semi-permeable membranes is a technology with high potential and in due time several new therapies based on this technology will be tested in clinical trials. Recent, new legislation requires that these investigational medicinal products used in clinical trials Phase I must be produced according to Good Manufacturing Practice (GMP). Consequently, the activities of GMP are expanding to the field of research and researchers might need to change developed protocols in order to meet GMP legislation. This chapters gives an overview of the overall guidelines covering GMP and more specific guidelines dealing with cell based therapies and gene therapy.</p
Regulatory Considerations in Application of Encapsulated Cell Therapies
No full textThe encapsulation of tissue in semi-permeable membranes is a technology with high potential and in due time several new therapies based on this technology will be tested in clinical trials. Recent, new legislation requires that these investigational medicinal products used in clinical trials Phase I must be produced according to Good Manufacturing Practice (GMP). Consequently, the activities of GMP are expanding to the field of research and researchers might need to change developed protocols in order to meet GMP legislation. This chapters gives an overview of the overall guidelines covering GMP and more specific guidelines dealing with cell based therapies and gene therapy.</p
Treatment of Diabetes with Encapsulated Islets
No full textCell encapsulation has been proposed for the treatment of a wide variety of diseases since it allows for transplantation of cells in the absence of undesired immunosuppression. The technology has been proposed to be a solution for the treatment of diabetes since it potentially allows a mandatory minute-to-minute regulation of glucose levels without side-effects. Encapsulation is based on the principle that transplanted tissue is protected for the host immune system by a semipermeable capsule. Many different concepts of capsules have been tested. During the past two decades three major approaches of encapsulation have been studied. These include (i) intravascular macrocapsules, which arc anastomosed to the vascular system as AV shunt, (ii) extravascular macrocapsules, which are mostly diffusion chambers transplanted at different sites and (iii) extravascular microcapsules transplanted in the peritoneal cavity. The advantages and pitfalls of the three approaches are discussed and compared in view of applicability in clinical islet transplantation.</p
Treatment of Diabetes with Encapsulated Islets
No full textCell encapsulation has been proposed for the treatment of a wide variety of diseases since it allows for transplantation of cells in the absence of undesired immunosuppression. The technology has been proposed to be a solution for the treatment of diabetes since it potentially allows a mandatory minute-to-minute regulation of glucose levels without side-effects. Encapsulation is based on the principle that transplanted tissue is protected for the host immune system by a semipermeable capsule. Many different concepts of capsules have been tested. During the past two decades three major approaches of encapsulation have been studied. These include (i) intravascular macrocapsules, which arc anastomosed to the vascular system as AV shunt, (ii) extravascular macrocapsules, which are mostly diffusion chambers transplanted at different sites and (iii) extravascular microcapsules transplanted in the peritoneal cavity. The advantages and pitfalls of the three approaches are discussed and compared in view of applicability in clinical islet transplantation.</p
Determination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin
No full textEulalia Sans-Serramitjana,1 Marta Jorba,1 José Luis Pedraz,2 Teresa Vinuesa,1 Miguel Viñas1 1Laboratory of Molecular Microbiology and Antimicrobials, Department of Pathology and Experimental Therapeutics, University of Barcelona, Barcelona, 2Laboratory of Pharmaceutics, University of the Basque Country (UPV/EHU), Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina, Vitoria, Spain Abstract: The emergence of colistin-resistant Pseudomonas aeruginosa in cystic fibrosis (CF) patients, particularly after long-term inhalation treatments, has been recently reported. Nanoencapsulation may enable preparations to overcome the limitations of conventional pharmaceutical forms. We have determined the time-dependent viability of P. aeruginosa biofilms treated with both free and nanoencapsulated colistin. We also examined the relationship between the optimal anti-biofilm activity of nanostructured lipid carrier (NLC)-colistin and the structural organization of the biofilm itself. The results showed the more rapid killing of P. aeruginosa bacterial biofilms by NLC-colistin than by free colistin. However, the two formulations did not differ in terms of the final percentages of living and dead cells, which were higher in the inner than in the outer layers of the treated biofilms. The effective anti-biofilm activity of NLC-colistin and its faster killing effect recommend further studies of its use over free colistin in the treatment of P. aeruginosa infections in CF patients. Keywords: cystic fibrosis, colistin sulfate, lipid nanoparticles, P. aeruginosa, confocal laser scanning microscopy, anti-biofilm activit
Immunomodulatory and Anti-inflammatory effect of Neural Stem/Progenitor Cells in the Central Nervous System
No full textNeuroinflammation is a critical event that responds to disturbed homeostasis and governs various neurological diseases in the central nervous system (CNS). The excessive inflammatory microenvironment in the CNS can adversely affect endogenous neural stem cells, thereby impeding neural self-repair. Therapies with neural stem/progenitor cells (NSPCs) have shown significant inhibitory effects on inflammation, which is mainly achieved through intercellular contact and paracrine signalings. The intercellular contact between NSPCs and immune cells, the activated CNS- resident microglia, and astrocyte plays a critical role in the therapeutic NSPCs homing and immunomodulatory effects. Moreover, the paracrine effect mainly regulates infiltrating innate and adaptive immune cells, activated microglia, and astrocyte through the secretion of bioactive molecules and extracellular vesicles. However, the molecular mechanism involved in the immunomodulatory effect of NSPCs is not well discussed. This article provides a systematic analysis of the immunomodulatory mechanism of NSPCs, discusses efficient ways to enhance its immunomodulatory ability, and gives suggestions on clinical therapy
Borax-loaded injectable alginate hydrogels promote muscle regeneration in vivo after an injury
No full text[EN] Muscle tissue possess an innate regenerative potential that involves an extremely complicated and synchronized process on which resident muscle stem cells play a major role: activate after an injury, differentiate and fuse originating new myofibers for muscle repair. Considerable efforts have been made to design new approaches based on material systems to potentiate muscle repair by engineering muscle extracellular matrix and/or including soluble factors/cells in the media, trying to recapitulate the key biophysical and biochemical cues present in the muscle niche. This work proposes a different and simple approach to potentiate muscle regeneration exploiting the interplay between specific cell membrane receptors. The simultaneous stimulation of borate transporter, NaBC1 (encoded by SLC4A11gene), and fibronectin-binding integrins induced higher number and size of focal adhesions, major cell spreading and actin stress fibers, strengthening myoblast attachment and providing an enhanced response in terms of myotube fusion and maturation. The stimulated NaBC1 generated an adhesion-driven state through a mechanism that involves simultaneous NaBC1/?5?1/?v?3 co-localization. We engineered and characterized borax-loaded alginate hydrogels for an effective activation of NaBC1 in vivo. After inducing an acute injury with cardiotoxin in mice, active-NaBC1 accelerated the muscle regeneration process. Our results put forward a new biomaterial approach for muscle repair.PR acknowledges support from the Spanish Ministry of Science, Innovation and Universities (RTI2018096794) , and Fondo Europeo de Desarrollo Regional (FEDER) . CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. The authors wish to thank also the intellectual and technical assistance from the ICTS "NANBIOSIS", more specifically by the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN) at the University of Basque Country (UPV/EHU) . The authors are grateful to A. Miralles for the credit of image of the mouse included in the graphical abstract.Ciriza, J.; Rodriguez-Romano, A.; Nogueroles, I.; Gallego-Ferrer, G.; Martín Cabezuelo, R.; Pedraz, JL.; Rico Tortosa, PM. (2021). Borax-loaded injectable alginate hydrogels promote muscle regeneration in
vivo after an injury. Materials Science and Engineering C: Materials for Biological Applications (Online). 123:1-14. https://doi.org/10.1016/j.msec.2021.112003S11412
Reconstruction of the main theoretical proposals regarding the problem of Scientific Science in Sports.
No full textEste artículo de revisión presenta una síntesis de los aportes histórico-culturales de siete de los más importan-tes autores (José María Cagigal, Jean le Boulch, Pierre, Parlebals, Miguel Vicente Pedraz, Herbert Haag, Manuel Sergio y Jaime Martínez) que argumentaron en favor de dar el calificativo de ciencia al campo denominado ciencias del deporte. Cada autor se examinó según cuatro categorías de análisis: nominación, objeto, método, cuerpos de conocimiento. Se presenta de forma descriptiva el panorama general. Se muestra la poca produc-ción en epistemología, la disparidad de nominaciones y los problemas taxonómicos que conllevan. Además, se presenta en una matriz la postura de cada autor. Se predice el desenlace positivo para la constitución de una ciencia, pero se advierte que este paso solo es posible si el análisis se da a la luz de la epistemología en rela-ción con el estado del arte de las ciencias del deporte.This review article presents a synthesis of the historical-cultural contributions of seven of the most important scholars (José María Cagigal, Jean le Boulch, Pierre, Parlebals, Miguel Vicente Pedraz, Herbert Haag, Manuel Sergio and Jaime Martínez) who argued in favor of giving the qualification of science to the field called sports sciences. Each author was examined according to four categories of analysis: nomination, object, method, bodies of knowledge. The overview is presented descriptively. It shows the low production in epistemology, the disparity of nominations and the taxonomic problems that they entail. In addition, the position of each author is presented in a matrix. The positive outcome for the constitution of a science is predicted, but it is noted that this step is only possible if the analysis is given in the light of epistemology in relation to the state of the art of sports science
Regulatory Considerations in Application of Encapsulated Cell Therapies
No full textThe encapsulation of tissue in semi-permeable membranes is a technology with high potential and in due time several new therapies based on this technology will be tested in clinical trials. Recent, new legislation requires that these investigational medicinal products used in clinical trials Phase I must be produced according to Good Manufacturing Practice (GMP). Consequently, the activities of GMP are expanding to the field of research and researchers might need to change developed protocols in order to meet GMP legislation. This chapters gives an overview of the overall guidelines covering GMP and more specific guidelines dealing with cell based therapies and gene therapy
- …
