8 research outputs found
Effect of Immunization Against Rubella on Lactation Products. II. Maternal-Neonatal Interactions
No full textEffect of Immunization Against Rubella on Lactation Products. I. Development and Characterization of Specific Immunologic Reactivity in Breast Milk
No full textEvaluation of a Neuraminidase-Specific Influenza A Virus Vaccine in Children: Antibody Responses and Effects on Two Successive Outbreaks of Natural Infection
No full textClinical and Immunologic Evaluation of NeuraminidaseSpecific Influenza A Virus Vaccine in Humans
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Individualised motivational counselling to enhance adherence to antiretroviral therapy is not superior to didactic counselling in South African patients: findings of the CAPRISA 058 randomised controlled trial.
Get PDFConcerns that standard didactic adherence counselling may be inadequate to maximise antiretroviral therapy (ART) adherence led us to evaluate more intensive individualised motivational adherence counselling. We randomised 297 HIV-positive ART-naïve patients in Durban, South Africa, to receive either didactic counselling, prior to ART initiation (n = 150), or an intensive motivational adherence intervention after initiating ART (n = 147). Study arms were similar for age (mean 35.8 years), sex (43.1 % male), CD4+ cell count (median 121.5 cells/μl) and viral load (median 119,000 copies/ml). Virologic suppression at 9 months was achieved in 89.8 % of didactic and 87.9 % of motivational counselling participants (risk ratio [RR] 0.98, 95 % confidence interval [CI] 0.90-1.07, p = 0.62). 82.9 % of didactic and 79.5 % of motivational counselling participants achieved >95 % adherence by pill count at 6 months (RR 0.96, 95 % CI 0.85-1.09, p = 0.51). Participants receiving intensive motivational counselling did not achieve higher treatment adherence or virological suppression than those receiving routinely provided didactic adherence counselling. These data are reassuring that less resource intensive didactic counselling was adequate for excellent treatment outcomes in this setting
Interleukin 7 from maternal milk crosses the intestinal barrier and modulates T-cell development in offspring
Get PDFBackground
Breastfeeding protects against illnesses and death in hazardous environments, an
effect partly mediated by improved immune function. One hypothesis suggests that
factors within milk supplement the inadequate immune response of the offspring,
but this has not been able to account for a series of observations showing that
factors within maternally derived milk may supplement the development of the
immune system through a direct effect on the primary lymphoid organs. In a
previous human study we reported evidence suggesting a link between IL-7 in
breast milk and the thymic output of infants. Here we report evidence in mice of
direct action of maternally-derived IL-7 on T cell development in the offspring.
Methods and Findings
We have used recombinant IL-7 labelled with a fluorescent dye to trace the
movement in live mice of IL-7 from the stomach across the gut and into the
lymphoid tissues. To validate the functional ability of maternally derived IL-
7 we cross fostered IL-7 knock-out mice onto normal wild type mothers. Subsets
of thymocytes and populations of peripheral T cells were significantly higher
than those found in knock-out mice receiving milk from IL-7 knock-out mothers.
Conclusions/Significance Our study provides direct evidence that interleukin 7,
a factor which is critical in the development of T lymphocytes, when maternally
derived can transfer across the intestine of the offspring, increase T cell
production in the thymus and support the survival of T cells in the peripheral
secondary lymphoid tissue.PLoS ON
Endogenous human cytomegalovirus gB is efficiently presented by MHC class II molecules to CD4+ CTL
No full textHuman cytomegalovirus (HCMV) infects endothelial, epithelial, and glial cells in vivo. These cells can express MHC class II proteins, but are unlikely to play important roles in priming host immunity. Instead, it seems that class II presentation of endogenous HCMV antigens in these cells allows recognition of virus infection. We characterized class II presentation of HCMV glycoprotein B (gB), a membrane protein that accumulates extensively in endosomes during virus assembly. Human CD4+ T cells specific for gB were both highly abundant in blood and cytolytic in vivo. gB-specific CD4+ T cell clones recognized gB that was expressed in glial, endothelial, and epithelial cells, but not exogenous gB that was fed to these cells. Glial cells efficiently presented extremely low levels of endogenous gB--expressed by adenovirus vectors or after HCMV infection--and stimulated CD4+ T cells better than DCs that were incubated with exogenous gB. Presentation of endogenous gB required sorting of gB to endosomal compartments and processing by acidic proteases. Although presentation of cellular proteins that traffic into endosomes is well known, our observations demonstrate for the first time that a viral protein sorted to endosomes is presented exceptionally well, and can promote CD4+ T cell recognition and killing of biologically important host cells
