625 research outputs found

    like the rames of mercy

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    rames n plHe was as thin as the rames of mercy. : said of a very thin person.YesDNE-cit SEP 1977[check]J. D. A. WIDDOWSON COLLECTOR'S PERMANENT FILEUsed INot usedUsed IThe second line of the quotation does not appear in the dictionary

    Enterovirus Database

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    Enterovirus reference sequence databases (EV_3kb and EV_7kb). Used for analysis of Enterovirus sequences, as described in Rames, E and Macdonald, J (2018) "Evaluation of MinION nanopore sequencing for rapid enterovirus genotyping", Submitted to Virus Research

    Des bases de l'activité vitale et de ses aptitudes / [signé Dr J. Rames]

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    De l'Action nerveuse (2e étude) par le Dr Rames,...

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    Microbial indicators related to yield and disease and changes in soil microbial community structure with ginger farm management practices

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    TRFLP (terminal restriction fragment length polymorphism) was used to assess whether management practices that improved disease suppression and/or yield in a 4-year ginger field trial were related to changes in soil microbial community structure. Bacterial and fungal community profiles were defined by presence and abundance of terminal restriction fragments (TRFs), where each TRF represents one or more species. Results indicated inclusion of an organic amendment and minimum tillage increased the relative diversity of dominant fungal populations in a system dependant way. Inclusion of an organic amendment increased bacterial species richness in the pasture treatment. Redundancy analysis showed shifts in microbial community structure associated with different management practices and treatments grouped according to TRF abundance in relation to yield and disease incidence. ANOVA also indicated the abundance of certain TRFs was significantly affected by farming system management practices, and a number of these TRFs were also correlated with yield or disease suppression. Further analyses are required to determine whether identified TRFs can be used as general or soil-type specific bio-indicators of productivity (increased and decreased) and Pythium myriotylum suppressiveness.E. K. Rames, M. K. Smith, S. D. Hamill, J. De Faver

    Aperçu sur le fonctionnement du système nerveux, par le Dr Rames,...

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    Considération sur le fonctionnement du système nerveux, par le Dr Rames

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    Rapid assessment of viral water quality using a novel recombinase polymerase amplification test for human adenovirus

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    Sensitive and rapid methods for determining viral contamination of water are critical, since illness can be caused by low numbers of viruses and bacterial indicators do not adequately predict viral loads. We developed novel rapid assays for detecting the viral water quality indicator human adenovirus (HAdV). A simple 15-min recombinase polymerase amplification step followed by a 5-min lateral flow detection is used. Species-specific assays were developed to discriminate HAdV A, B, C and F, and combined into a multiplex test (Ad-FAC). Species-specific assays enabled detection of 10–50 copies of the HAdV plasmid. Sample testing using methods optimised for wastewater analysis indicated the Ad-FAC assay showed 100% sensitivity and 100% specificity when compared with HAdV qPCR, with a detection limit as low as 50 gene copies. This is the first study to demonstrate the use of RPA for detecting enteric viruses in water samples, to assess virological water quality. The ability to rapidly detect enteric virus contamination of water could assist in more effective management of water safety and better protection of public health.No Full Tex
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