186 research outputs found
Assignment of bovine submaxillary mucin (BSM1) gene homologues to bubaline, caprine, and ovine chromosomes by comparative mapping.
No full textKaryotype, centric fusion polymorphism and chromosomal aberrations in captive-born mountain reedbuck (Redunca fulvorufula).
No full textChromosomes of fourteen captive-born mountain reedbucks (Redunca fulvorufula) have been investigated. The diploid chromosome number was 2n = 56 (FN = 60). The mountain reedbuck karyotype consists of 26 acrocentric and two biarmed chromosome pairs resulting from two centric fusions involving chromosomes 2 and 25, and 6 and 10, respectively. In some animals, 57 chromosomes were detected. Variation in the diploid number was found to be due to polymorphism for the centric fusion 6;10. Both X and Y chromosomes are large and acrocentric. The entire Y chromosome and the proximal part of the X chromosome consist of heterochromatin. The chromosomes X, 9 and 14 appeared to be of caprine type. Chromosome aberrations have been detected in two of the 14 animals investigated. A de novo formed Robertsonian translocation rob(6;13) was found in one female heterozygous for the fusion 6;10. CBG-banding revealed one block of centromeric heterochromatin in the de novo formed translocation rob(6;13) and also in the evolutionarily fixed centric fusions 6;10 and 2;25. One examined male homozygous for fusion 6;10, had a mosaic 56,XY/57,XYY karyotype, with 11% of analyzed cells containing two Y chromosomes. The findings were confirmed by cross-species fluorescence in situ hybridization (FISH) with bovine (Bos taurus L.) chromosome painting probes. The study demonstrates the relevance of cytogenetic screening in captive animals from zoological gardens
The porcine proteasome subunit A4 (PSMA4) gene: Isolation of a partial cDNA, linkage and physical mapping
No full textA partial cDNA clone encoding the porcine proteasome subunit A4 (PSMA4 or proteasome subunit C9) has been isolated from a porcine muscle cDNA library and sequenced. A biallelic TaqI RFLP was identified in Large White, Landrace and Duroc breeds. Moreover, the 3'-untranslated region of the gene showed a triallelic SSCP. By linkage analysis the PSMA4 locus was assigned to pig chromosome 7 and by radioactive in situ hybridization this locus was mapped to the region 7q13-q14
Assignment of the troponin C2 fast gene (TNNC2) to porcine chromosome bands 17q2.1· q2.2 by in situ hybridization
No full textAssignment of troponin C2, fast (TNNC2) gene to porcine chromosome bands 17q21-q22 by in situ hybridizatio
Isolation and physical localization of new chromosome-specific centromeric repeats in farm animals
No full textChromosomal unbalancements in sperm and oocytes of two Italian cattle breeds as determined by dual color fluorescent in situ hybridization (FISH)
No full textAneuploidy is one of the most important causes of embryonic and foetal mortality in mammals. In order to assess the possible risk of chromosomal abnormalities in germ cells of domestic animals we investigated the aneuploidy rates on partially decondensed sperm and in vitro matured oocytes in two cattle breeds, Italian Friesian (I.F.) and Italian Brown (I.B.), by using FISH with chromosome-specific painting probes (chromosomes X-Y for sperm and chromosomes X-5 for oocytes). For each bull, more than 5,000 sperm were analyzed, for a total of 52,586 and 51,342 sperm cells for the two breeds, respectively. Aneuploid and diploid sperm had, respectively, a frequency of 0.110% and 0.050% in the I.F. and 0.078% and 0.062% in the I.B. breeds. Out of 100 in vitro matured oocytes for each breed, on the average, diploidy affected 11.2% and 18.4% in the I.F. and I.B., respectively, whereas disomy for chromosome X-had a frequency of 2% in the I.F. and 2.5% in the I.B. breeds. Further studies are needed to expand our knowledge on frequency of aneuploidy in sperm and oocytes of domestic animals, in order to assess their impact on productive and reproductive efficiency, also in relation to climatic changes and environmental hazards
The porcine poly(rC)-binding protein 2 (PCBP2) gene maps to chromosome 5
No full textA recombinant clone was isolated at random from a skeletal muscle cDNA library. The sequence of the resulting 598 bp insert (EMBL accession number X94253) was compared in EMBL and GenBank databases using BLASTN2. The comparisons showed the highest matches with mouse and human PCBP2 cDNAs (mouse X75947, mouse X97982, human X78136 and mouse L19661; BLASTN probability were 1·9e-182, 1·1e-176, 1·6e-174 and 4·7 e-172, respectively). Excluding two gaps that could be due to alternative splicing, the deduced porcine protein sequence revealed the complete identity with corresponding human ( X78136) and mouse ( L19661) sequences. The mouse sequences X75947and X97982showed only one amino acid difference (Arg instead of Gly) with the deduced amino acid sequence of the porcine cDNA clone isolated. The cDNA was used as probe for in situ hybridization and for RFLP analysis in three generation reference populations. Both approaches assigned this gene to porcine chromosome 5
Recent developments on genetic information among river type buffaloes by chromosome microdissection and cloning
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Phylogenomic study of the subfamily Tragelapinae by cross-species chromosome painting with cattle paints
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