202 research outputs found
Affect on forecasting reliability safety engine in medical institutions; International Conference of Experimental and Numerical Investigations and NewTechnologies
No full textM. Ristic, Lj. Radovanovic, J. Perisic, I. Vasovic, G. Otic; Affect on forecasting reliability safety engine in medical institutions; International Conference of Experimental and Numerical Investigations and NewTechnologies; Zlatibor, Serbia, 29 Jun- 02 July 2021; Serbia; The book of Abstracts; ISBN: 978-86-6060-077-8; pp. 5; 202
Abstract A32: The role of the ribosome in GCN2's ability to sense amino acid deprivation and initiate the integrated stress response
No full textAbstract
The ability to replicate without limit is one of the traditional hallmarks of cancer. It is therefore evident that the majority of tumor cells are operating in environments with limited nutrients. This means that tumor cells are disproportionately reliant on their stress response pathways in comparison to healthy cells. One such stress response pathway is a component of the integrated stress response pathway that responds to deprivation of amino acids. GCN2 is a serine/threonine protein kinase that is activated in response to a drop in the intracellular concentration of amino acids. Upon activation, GCN2 phosphorylates the translational initiation factor eIF2, inhibiting its activity in the translational cycle. This leads to a global inhibition of translation, whilst paradoxically upregulating the expression of certain genes involved in stress responses. It has been shown that knocking out GCN2 leads to a significant reduction in the growth of tumors in mice, suggesting that GCN2 could be a useful therapeutic target.
We have expressed and purified human GCN2 and found it to be a dimer, as has been observed for yeast GCN2. We have shown in vitro that GCN2 becomes autophosphorylated, and it catalyses phosphorylation of a putative binding partner GCN1 as well as eIF2. We have found that human GCN2 forms a stable complex with 80S ribosomes, and mammalian 80S ribosomes significantly increase GCN2 kinase activity in vitro, consistent with a previous report that an increase in ribosomal stalling leads to a rise in eIF2 phosphorylation in vivo. We are attempting to determine the structure of GCN2 bound to ribosomes in order to understand the detailed interactions and the molecular mechanisms that lead to GCN2 activation.
Citation Format: Alison J. Inglis, Sichen Shao, Glenn R. Masson, Olga Perisic, Ramanujan S. Hegde, Roger L. Williams. The role of the ribosome in GCN2's ability to sense amino acid deprivation and initiate the integrated stress response. [abstract]. In: Proceedings of the AACR Special Conference on Translational Control of Cancer: A New Frontier in Cancer Biology and Therapy; 2016 Oct 27-30; San Francisco, CA. Philadelphia (PA): AACR; Cancer Res 2017;77(6 Suppl):Abstract nr A32.</jats:p
Pretrial detention and alternate methods of securing the presence of the accused during the criminal procedure
No full textIn this paper, the author discusses pretrial detention and alternate methods of securing the presence of the accused during the criminal procedure. Except issues of terminology, in the introduction to this paper, the author deals with the current status of our criminal justice system, the readiness of both the state and the criminal justice system to accept innovations. Further, the author examines the accordance of pretrial detention with international sources, then its correlations with the Constitution, Criminal Procedure Codes from 2001 and 2006, and types of pretrial detention. As the paper continues, it describes institutes that are alternatives of pretrial detention: home detention and other restriction orders, and bail. The paper follows with the definition and content of the reasonable doubt concept as a mandatory condition for pronouncing pretrial detention. The major part of the paper deals is dedicated to the discussion of legal regulations of pretrial detention and its misinterpretation by judicial apparatus, and the effort to explain some undefined parts of those regulations
Harmonized Translation: A Case Study from English to Serbian
No full textHarmonized translation is not just a process of translation; it is a creative endeavor that transcends simple language conversion. It's a sophisticated method that artfully interweaves the original song’s melody, rhythm, and poetic essence with the target language. This results in a seamless and expressive rendition that resonates with its new audience while preserving the original's musical integrity, emotional resonance, and contextual relevance. In essence, harmonized translation is a profound way of connecting languages and cultures through the power of music, inspiring a new level of creativity in the translation process. Compared to traditional translation theories, which encompass lyrics and poems as discussed by Munday, J. (2016), harmonized translation emphasizes measurable qualities necessary for achieving what can succinctly be described as fidelity
Protease-activated kinase II as the mediator of epidermal growth factor-stimulated phosphorylation of ribosomal protein S6
No full textAbstractEpidermal growth factor stimulates phosphorylation of ribosomal protein S6 in serum-starved Swiss 3T3 cells, leading to the formation of highly phosphorylated derivatives containing 4–5 phosphates. Two-dimensional analysis of tryptic phosphopeptides of S6 shows an identical pattern to the ones obtained previously in other cells in response to insulin and the tumor promoting phorbol ester, 12-O-tetradecanoyl phorbol-13-acetate. This suggests a common intracellular mediator of S6 phosphorylation by different growth promoting agents. It is proposed that the potential mediator of this phosphorylation is the Ca2+-independent, cAMP-independent protein kinase, protease activated kinase II, as shown by the extent of phosphorylation and the tryptic phosphopeptide maps of S6 with highly purified enzyme [(1983) J. Biol. Chem. 258, 13998-14002]
OVERCOMING EMPTINESS IN THE TRANSLATION OF DUBRAVKA UGREŠIĆ
No full textThe theme of this paper concerns the main aspects of translating a linguistically and culturally hybrid text, using the example of two Dubravka Ugrešić’s books (1949–2023), Karaoke culture and Europe in Sepia. The author addresses contemporary themes in a globalised and increasingly virtual world, while constantly revisiting the Soviet and Yugoslav communist past through her personal experiences. Existing between languages and interpreting such a diverse literary world requires a profound understanding of historical, linguistic, and cultural contexts, as well as translation strategies aimed at bridging the inevitable spatiotemporal gap that arises between the two versions of the text. The main research questions are: what are the criteria for including or excluding different elements of heterolinguism/ plurilingualism in a translated text? By what linguistic means is it domesticated in a culturally and linguistically hybrid text
Afferent connections of the ectostriatum and visual wulst in the zebra finch (Taeniopygia guttata castanotis Gould) — an HRP study
No full textNixdorf B, Bischof H-J. Afferent connections of the ectostriatum and visual wulst in the zebra finch (Taeniopygia guttata castanotis Gould) — an HRP study. Brain Research. 1982;248(1):9-17.Afferent connections of the two main areas in the telencephalon, the visual wulst and the ectostriatum, were traced in the zebra finch by injection of horseradish peroxidase and staining with tetramethylbenzidine (TMB). Nuclei projecting to the hyperstriatum accessorium (HA) or the HIS region (lamina hyperstriatica intercalatus superior) were: (1) ipsilaterally the n. dorsalis anterior pars lateralis (DLL) with its two subdivisions DLLd and DLLv, the n. dorsolateralis anterior pars magnocellularis (DLAmc), and the area pretectalis (AP); (2) bilaterally the nucleus of the septomesencephalic tract (SPC) with the ipsilateral component coming from the medial, the contralateral component from the lateral part of the nucleus. As in the pigeon or the owl the ectostriatum of the zebra finch receives massive input, which is topographically ordered, from the n. rotundus. In addition to this pathway the ectostriatum receives additional visual input from the ipsilateral area pretectalis, the n. subrotundus and eventually a bilateral projection from the n. tegmenti pedunculopontinus pars compacta (TPC)
Lighthouses as a Part of Tourist Offer Being Specific Tourist
No full textThis paper presents Croatian lighthouses as a unique tourist category on Croatian and European tourist market and its contribution to Croatian tourist offer in generallighthouse, tourist offer
Supporting student agency and success in higher education and beyond through the development of assessment feedback skills (the ability to self-monitor and self-evaluate)
No full textA possible role of repressive chromatin marks in GLT-1 unresponsivity to dexamethasone in rat cerebellar astrocytes
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