144 research outputs found

    [<em>Pasteurella</em>] <em>caballi</em> infection not limited to horses - a closer look at taxon 42 of Bisgaard

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    Aim To investigate if taxon 42 of Bisgaard isolated from pigs represents genuine [Pasteurella] caballi which has previously only been isolated from horses. Methods and Results A total of 15 field isolates from horses and pigs from 5 different countries representing three continents were to subjected extended phenotypical characterization. Although minor differences were observed between taxon 42 and [P.] caballi, these differences did not allow phenotypic separation. Ribotyping based on HindIII digestion showed five profiles based on 9 band positions. One [P.] caballi strain and two taxon 42 strains shared the same profile. Ribotyping using HpaII gave a higher diversity with 9 profiles based on 10 band positions. While no profiles were shared between the Taxon 42 and [P.] caballi strains, pattern analysis showed that two of the Taxon 42 isolates were most similar (91 % similarity) with a [P.] caballi isolate. 16S rRNA gene sequencing of one strain of taxon 42 and one strain of [P.] caballi was performed and compared to the published sequence for the type strain of [P.] caballi. The three strains showed nearly identical sequences with at least 99.8 % similarity. DNA re-associations measured by the micro-well method were 79 and 77 %, respectively between the type strain of [P.] caballi and two strains of taxon 42 representing distinct ribotypes and confirmed that taxon 42 belongs to [P.] caballi. Conclusion The present investigation documents that [P.] caballi can be isolated from clinical respiratory specimens from pigs as well as the recognised association with respiratory infections in horses and horse bite infection in humans. Strains classified as taxon 42 are [P.] caballi isolated from pigs and for both pigs and horses lesions mainly include the respiratory tract. Significance and impact of study The results will improve the diagnostics and progress studies of virulence and epidemiology of [P.] caballi

    Pasteurella aerogenes Isolations from Swine

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    A simple identification scheme for coagulase-negative Staphylococci from bovine mastitis

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    Coagulase-negative staphylococci from cases of bovine mastitis were identified to species level by using an identification scheme based on a three-plate test system which tested for DNase on DNA agar, for protease on calcium caseinate agar, and for the organism's sensitivity to novobiocin, desferrioxaminine (deferoxamine) and fosfomycin by agar diffusion tests. Testing for the inhibition of Staphylococcus delta haemolysin (Skalka 1991) can replace the protease tests
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