1,721,024 research outputs found
The effects of corticotrophin (ACTH1-24), cyclic AMP and TPA (12-O-tetradecanoyl phorbol-13-acetate) on DNA replication and proliferation of primary rabbit adrenocortical cells in a synthetic medium
No full textACTH1-24 stimulated the parenchymal cells in cultures of rat adrenal cortex in serum-free synthetic HiWoBa 2000 medium to replicate DNA, enter mitosis and divide. But ACTH's principal mediator, cyclic AMP, was not a complete mitogen: the adenylate cyclase-stimulating cholera toxin and dibutyryl cyclic AMP stimulated parenchymal cells to replicate DNA but not to enter mitosis. Thus, there must have been an additional mediator of the response to ACTH1-24 that enabled the parenchymal cells to enter mitosis. This additional mediator might have been protein kinase C because a protein kinase C activator and cyclic AMP elevator, TPA, stimulated the adrenocortical parenchymal cells to replicate DNA, enter mitosis and divide
The effect of x-irradiation on the nicotinamide adenine dinucleotides (NAD-NADH) content of rat thymocytes
No full textStimulation of DNA synthesis and mitosis of hepatocytes in primary cultures of neonatal rat liver by arachidonic acid and prostaglandins A1, E1, and E2.
No full textIt is shown that the stimulation of DNA synthesis and mitosis of hepatocytes is elicited in primary cultures of neonatal rat liver by arachidonic acid and prostaglandins A1, E1, and E2
Stimulation of DNA synthesis and mitosis of hepatocytes in primary cultures of neonatal rat liver by arachidonic acid and prostaglandins.
No full textAt low concentrations (i.e. 10−12–10−9 mol/l) arachidonic acid intensely stimulated both DNA synthetic and mitotic activities of hepatocytes in 4-day-old primary cultures of neonatal rat liver. This effect of arachidonate was completely suppressed by the simultaneous administration to the cultures of a high dose (i.e. 10−4 mol/l) of indomethacin. A similar, but much weaker proliferogenic activity was exerted on neonatal hepatocytes by quite low concentrations of some of the main products of arachidonic acid metabolism, namely prostaglandins A1, E1, and E2. Although these data support the possibility that arachidonate and prostaglandins are involved in the regulation of hepatocytic proliferative activation, the exact role of prostaglandins remains to be ascertained, because such agents might as well have acted by inducing intracellular surges of known mitogenic compounds, such as cAMP and cGMP
Arachidonic acid and prostaglandins stimulate the growth of primary neonatal rat hepatocytes
No full textArachidonic acid and prostaglandins stimulate the growth of primary neonatal rat hepatocytes in vitr
The calcium-dependence of the stimulation of neonatal rat hepatocyte DNA synthesis and division by epidermal growth factor, glucagon and insulin
No full textA low concentration (10(-11) mol/l) of epidermal growth factor (EGF) and/or an equimolar (10(-14) mol/l) mixture of glucagon and insulin stimulated DNA synthesis in hepatocytes in 4-day-old primary cultures of neonatal rat liver. EGF seems to have acted by inducing quiescent hepatocytes to begin cycling, while the glucagon-insulin combination seems to have acted mainly by shortening the cell cycle time. Incubation in low calcium medium blocked untreated hepatocytes in the G1 phase of their cycle and prevented EGF and the glucagon-insulin mixture from stimulating DNA synthesis. Nevertheless, hepatocytes in calcium-deficient medium did respond to these agents, as they reached a late stage of prereplicative development before being blocked: in fact, they initiated DNA synthesis soon after the addition of calcium. EGF, but not the glucagon-insulin combination, also enabled the already cycling hepatocytes (but not the newly activated ones) to overcome the block imposed by the extracellular calcium deficiency after a delay of several hours
The effects of the novel human parathyroid hormone fragment, hPTH-(1-31)NH2, on the proliferation and differentiation of cultured human mandibular osteoblasts.
No full textThe effects of the novel human parathyroid hormone fragment, hPTH-(1-31)NH2, on the proliferation and differentiation of cultured human mandibular osteoblasts
Arachidonic acid and prostaglandins stimulate DNA synthesis and mitosis in neonatal rat hepatocytes set into primary tissue culture.
No full textArachidonic acid and prostaglandins stimulate DNA synthesis and mitosis in neonatal rat hepatocytes set into primary tissue cultur
The calcium-dependent stimulation of the proliferation of neonatal rat hepatocytes by imidazole and indomethacin: Chem.Biol.Interact.
No full textNRC publication: Ye
Parathyroid hormone-related protein (PTH-rP) and the control of the growth and differentiation of normal human keratinocytes.
No full textParathyroid hormone-related protein (PTH-rP) and the control of the growth and differentiation of normal human keratinocytes
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