13,793 research outputs found
Oncolytic adenovirus co-expressing IL-12 and IL-18 improves tumor-specific immunity via differentiation of T cells expressing IL-12R beta(2) or IL-18R alpha
No full textThe oncolytic adenovirus (Ad) is currently being advanced as a promising antitumor remedy as it selectively replicates in tumor cells and can transfer and amplify therapeutic genes. Interleukin (IL)-12 induces a potent antitumor effect by promoting natural killer (NK) cell and cytotoxic T cell activities. IL-18 also augments cytotoxicity of NK cells and proliferation of T cells. This effect further enhances the function of IL-12 in a synergistic manner. Therefore, we investigated for the first time an effective cancer immunogene therapy of syngeneic tumors via intratumoral administration of oncolytic Ad co-expressing IL-12 and IL-18, RdB/IL-12/IL-18. Intratumoral administration of RdB/IL-12/IL-18 improved antitumor effects, as well as increased survival, in B16-F10 murine melanoma model. The ratio of T-helper type 1/2 cytokine as well as the levels of IL-12, IL-18, interferon-gamma and granulocyte-macrophage colony-stimulating factor was markedly elevated in RdB/IL-12/IL-18-treated tumors. Mice injected with RdB/IL-12/IL-18 also showed enhanced cytotoxicity of tumor-specific immune cells. Consistent with these results, immense necrosis and infiltration of NK cells, as well as CD4(+) and CD8(+) T cells, were observed in RdB/IL-12/IL-18-treated tumor tissues. Importantly, tumors treated with RdB/IL-12/IL-18 showed an elevated number of T cells expressing IL-12R beta 2 or IL-18R alpha. These results provide a new insight into therapeutic mechanisms of IL-12 plus IL-18 and provide a potential clinical cancer immunotherapeutic agent for improved antitumor immunity.This work was supported by grants from the Ministry of Knowledge Economy (10030051, Dr C-O Yun), the Korea Science and Engineering Foundation (R15-2004-024-02001-0, 2009K001644, 2010-0029220, Dr C-O Yun), the Korea Food and Drug Administration (KFDA-10172-332 to Dr C-O Yun), and Yonsei University College of Medicine faculty research grant (6-2010-0052, Dr C-O Yun). I-K Choi is a graduate student sponsored by KOSEF through National Core Research Center for Nanomedical Technology. J-S Lee and S-N Zhang are graduate students sponsored by the Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea
FIGURE 1. Melampyrum koreanum K.-J. Kim & S.-M. Yun. A. Flowering branches. B. Upper corolla lobes and stamens. C. Developing flowers. D. Mature flower. E. Calyx and style. F. Lower leaf. G–H. Upper bract-like leaves. I–J. Capsules. K. Seeds. All scale bars are 1 in A new species of Melampyrum (Orobanchaceae) from Southern Korea
No full textFIGURE 1. Melampyrum koreanum K.-J. Kim & S.-M. Yun. A. Flowering branches. B. Upper corolla lobes and stamens. C. Developing flowers. D. Mature flower. E. Calyx and style. F. Lower leaf. G–H. Upper bract-like leaves. I–J. Capsules. K. Seeds. All scale bars are 1 cm. Drawn from the holotype K.-J. Kim and Seok Min Yun 2008-1561(KUS).Published as part of Kim, Ki-Joong & Yun, Seok-Min, 2012, A new species of Melampyrum (Orobanchaceae) from Southern Korea, pp. 48-50 in Phytotaxa 42 on page 49, DOI: 10.11646/phytotaxa.42.1.6, http://zenodo.org/record/489483
A functional variant in the promoter region regulates the C-reactive protein gene and is a potential candidate for increased risk of atrial fibrillation
No full text. Chang S-N, Tsai C-T, Wu C-K, Lee J-K, Lai L-P, Huang S-W, Huang L-Y, Tseng C-D, Lin J-L, Chiang F-T, Hwang J-J (National Taiwan University Hospital Yun-Lin Branch, Yun-Lin; National Taiwan University Hospital, Taipei; National Taiwan University Hospital, Taipei; Institute of Pharmacology, Taipei; and Graduate Institute of Biomedical Engineering, Taipei, Taiwan). A functional variant in the promoter region regulates the C-reactive protein gene and is a potential candidate for increased risk of atrial fibrillation. J Intern Med 2012; 272: 305315. Objectives. In a large population-based cohort, the level of C-reactive protein (CRP) in patients at baseline predicts an increased risk of future development of atrial fibrillation (AF). The mechanism of this increased risk is unknown. Furthermore, both the molecular effects of CRP on atrial myocytes and fibroblasts and whether genetic variants in the CRP gene predispose to AF are also unknown. Methods.similar to A genetic association study between CRP gene polymorphisms and AF was performed in two independent populations (I: 100 AF patients and 101 controls; II: 348 AF patients and 356 controls), with functional studies to elucidate the mechanism of association. Results.similar to Three polymorphisms (T-861C, A-821G and C-390A/C-390T) were found in the 1-kb promoter of CRP. A triallelic polymorphism (C-390A/C-390T) captured all haplotype information and determined the CRP gene promoter activity and the plasma CRP level, and was in nearly complete linkage disequilibrium with G1059C polymorphism in exon 2. The -390A variant was associated with a higher CRP gene promoter activity, a higher plasma CRP level and a higher risk of AF. Patients with AF also had a higher plasma CRP level than controls. CRP significantly increased the inward L-type calcium current in atrial myocytes with no changes in other ionic currents. CRP did not affect the expressions of type I alpha 1 (COL1A1), type III alpha 1 (COL3A1) and type 1 alpha 2 (COL1A2) procollagens in atrial fibroblasts. Conclusion.similar to A CRP gene promoter triallelic polymorphism was associated with CRP gene promoter activity, determined the plasma level of CRP, and predicted the risk of AF. The mechanism of this may be via augmention of calcium influx by CRP in atrial myocytes, but not because of atrial fibrosis
Interrogation of fiber grating sensor arrays with a wavelength-swept fiber laser
No full textWe demonstrate a novel application of a wavelength-swept fiber laser to fiber Bragg grating sensor-array interrogation. The laser provides high signal powers of >3 mW with <0.1-nm spectral resolution over a 28-nm wavelength span. Using time-interval counting, we demonstrate static-dynamic strain measurements with a resolution of 0.47 mu epsilon rms at a sampling rate of 250 Hz. (C) 1998 Optical Society of America.S. H. Yun acknowledges the financial support of
the Korea Research Foundation in program year 1997.
D. J. Richardson was supported by the Royal Society
under the provision of a Royal University Fellowship
A Study of Aerodynamics in Agriculture
No full textRosana G. Moreira, Editor-in-Chief; Texas A&M UniversityThis is an Invited Paper from International Commission of Agricultural Engineering (CIGR, Commission Internationale du Genie Rural) E-Journal Volume 5 (2003): I. Lee, C. Kang, J. Yun, J. Jeun, and G. Kim. A Study of Aerodynamics in Agriculture. Vol. V. December 2003
Predicting photoisomerization profile of the highly polymerized nematic azobenzene liquid crystal network: First principle calculation
No full textThe cis profile of azobenzene is a key factor in predicting the photodeformation of the nematic azobenzene liquid crystal network (LCN). An ab initio based method for predicting the photoisomerization profile of azobenzene is developed by coupling the stimulated Raman adiabatic passage (STIRAP) method with nonlinear Beers law, and compared with experimental data. Using this combined method, we calculate the photoisomerization profile of azobenzene with various light input conditions. We identify the cis profile of the nematic LCN structure evolves into a step-like decaying shape when the direction of polarized light is parallel to the nematic direction. (C) 2015 Published by Elsevier B.V.This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2012R1A3A2048841)
Jia ru ju he wu dui jun yun tuan liu ji jun yun tuan liu dui liu de ying xiang
No full textWong, Chai Kwok = 加入聚合物對均勻湍流及均勻湍流對流的影響 / 黃濟國.Thesis M.Phil. Chinese University of Hong Kong 2013.Includes bibliographical references (leaves 89-91).Abstracts also in Chinese.Title from PDF title page (viewed on 01, November, 2016).Wong, Chai Kwok = Jia ru ju he wu dui jun yun tuan liu ji jun yun tuan liu dui liu de ying xiang / Huang Jiguo
Recommended from our members
Macroeconomic models and the yield curve: An assessment of the fit
No full textMany have questioned the empirical relevance of the Calvo-Yun model. This paper adds a term structure to three widely studied macroeconomic models (Calvo-Yun, hybrid and Svensson). We back out from observations on the yield curve the underlying macroeconomic model that most closely matches the level, slope and curvature of the yield curve. With each model we trace the response of the yield curve to macroeconomic shocks. We assess the fit of each model against the observed behaviour of interest rates and find limited support for the Calvo-Yun model in terms of fit with the observed yield curve, we find some support for the hybrid model but the Svensson model performs best
Enhancing the therapeutic efficacy of adenovirus in combination with biomaterials
No full textWith the reason that systemically administered adenovirus (Ad) is rapidly extinguished by innate/adaptive immune responses and accumulation in liver, in vivo application of the Ad vector is strictly restricted. For achieving to develop successful Ad vector systems for cancer therapy, the chemical or physical modification of Ad vectors with polymers has been generally used as a promising strategy to overcome the obstacles. With polyethylene glycol (PEG) first in order, a variety of polymers have been developed to shield the surface of therapeutic Ad vectors and well accomplished to extend circulation time in blood and reduce liver toxicity. However, although polymer-coated Ads can successfully evacuate from a series of guarding systems in vivo and locate within tumors by enhanced permeability and retention (EPR) effect, the possibility to entering into the target cell is few and far between. To endow targeting moiety to polymer-coated Ad vectors, a diversity of ligands such as tumor-homing peptides, growth factors or antibodies, have been introduced with avoiding unwanted transduction and enhancing therapeutic efficacy. Here, we will describe and classify the characteristics of the published polymers with respect to Ad vectors. Furthermore, we will also compare the properties of variable targeting ligands, which are being utilized for addressing polymer-coated Ad vectors actively.This work was supported by NIH CA107070 (Sung Wan Kim), the Ministry of Knowledge Economy (10030051, C-O. Yun), the Korea Science and Engineering Foundation (2009K001644, 2010-0029220, C-O Yun), and the Korea Food and Drug Administration (KFDA-10172-332 to C-O Yun)
FIGURE 2. Hemiboea malipoensis. A–B. Habit. C. Involucre. D. Involucel and opened involucel. E. Opened corolla showing stamens and staminode. F. Stamens. G. Calyx and pistil with disc. I. Calyx. H. Stigma. J in Hemiboea malipoensis, a new species of Gesneriaceae from southeastern Yunnan, China
No full textFIGURE 2. Hemiboea malipoensis. A–B. Habit. C. Involucre. D. Involucel and opened involucel. E. Opened corolla showing stamens and staminode. F. Stamens. G. Calyx and pistil with disc. I. Calyx. H. Stigma. J. Fruit. Illustration by Yun-Xi Zhu based on the holotype Yun-Hong Tan 6055.Published as part of Zhang, Li-Xia, Tan, Yun-Hong, Li, Jian-Wu, Wen, Bin, Yin, Jian-Tao & Lan, Qin-Ying, 2014, Hemiboea malipoensis, a new species of Gesneriaceae from southeastern Yunnan, China, pp. 165-172 in Phytotaxa 174 (3) on page 168, DOI: 10.11646/phytotaxa.174.3.5, http://zenodo.org/record/515062
- …
