89 research outputs found
First clinical assessment of a blood glucose predictor and therapy advisor in type 1 diabetes: The DIAdvisor-1 Trial
No full text
SPLEEN REGENERATION IN MICE AFTER GAMMA IRRADIATION AND ADMINISTRATION OF THYMOSIN
No full textThe effect of thymosin (thymic humeral factor isolated from calf thymus) on regeneration of the spleen in mice after whole-body gamma irradiation was studied. Thymosin, in varied dosages (0.1-2.0 mg/day) applied subcutaneously before and after radiation exposure, stimulated splenic regeneration as indicated by increased splenic weight, number of endogenous splenic colonies and 59Fe and 125IUdR incorporation into the spleen. A control extract of brain tissue (cerebrosin) isolated in the same way as thymosin was applied to mice to verify specificity of thymosin. After cerebrosin application, a mild increase also was observed. Whereas a near maximal effect of thymosin was reached at a dosage of 0.1 mg, a comparable response with cerebrosin required a dosage of 1.0 mg. These data suggest that administration of thymosin has both a specific and nonspecific effect on splenic regeneration and proliferation of hematopoietic stem cells
Distribution of human beta-defensin polymorphisms in various control and cystic fibrosis populations.
No full textAbstract
Human beta defensins contribute to the first line of defense against infection of the lung. Polymorphisms in these genes are therefore potential modifiers of the severity of lung disease in cystic fibrosis. Polymorphisms were sought in the human beta-defensin genes DEFB1, DEFB4, DEFB103A, and DEFB104 in healthy individuals and cystic fibrosis (CF) patients living in various European countries. DEFB1, DEFB4, and DEFB104 were very polymorphic, but DEFB103A was not. Within Europe, differences between control populations were found for some of the frequent polymorphisms in DEFB1, with significant differences between South-Italian and Czech populations. Moreover, frequent polymorphisms located in DEFB4 and DEFB104 were not in Hardy Weinberg equilibrium in all populations studied, while those in DEFB1 were in Hardy Weinberg equilibrium. Sequencing of a monochromosomal chromosome 8 mouse-human hybrid cell line revealed signals for multiple alleles for some loci in DEFB4 and DEFB104, but not for DEFB1. This indicated that more than one DEFB4 and DEFB104 gene was present on this chromosome 8, in agreement with recent findings that DEFB4 and DEFB104 are part of a repeat region. Individual DEFB4 and DEFB104 PCR amplification products of various samples were cloned and sequenced. The results showed that one DNA sample could contain more than two haplotypes, indicating that the various repeats on one chromosome were not identical. Given the higher complexity found in the genomic organization of the DEFB4 and DEFB104 genes, association studies with CF lung disease severity were performed only for frequent polymorphisms located in DEFB1. No association with the age of first infection by Pseudomonas aeruginosa or with the FEV1 percentage at the age of 11-13 years could be found
Distribution of beta-defensin polymorphisms in various control and cystic fibrosis populations
No full textHuman β defensins contribute to the first line of defense against infection of the lung. Polymorphisms in these genes are therefore potential modifiers of the severity of lung disease in cystic fibrosis. Polymorphisms were sought in the human β-defensin genes DEFB1, DEFB4, DEFB103A, and DEFB104 in healthy individuals and cystic fibrosis (CF) patients living in various European countries. DEFB1, DEFB4, and DEFB104 were very polymorphic, but DEFB103A was not. Within Europe, differences between control populations were found for some of the frequent polymorphisms in DEFB1, with significant differences between South-Italian and Czech populations. Moreover, frequent polymorphisms located in DEFB4 and DEFB104 were not in Hardy Weinberg equilibrium in all populations studied, while those in DEFB1 were in Hardy Weinberg equilibrium. Sequencing of a monochromosomal chromosome 8 mouse-human hybrid cell line revealed signals for multiple alleles for some loci in DEFB4 and DEFB104, but not for DEFB1. This indicated that more than one DEFB4 and DEFB104 gene was present on this chromosome 8, in agreement with recent findings that DEFB4 and DEFB104 are part of a repeat region. Individual DEFB4 and DEFB104 PCR amplification products of various samples were cloned and sequenced. The results showed that one DNA sample could contain more than two haplotypes, indicating that the various repeats on one chromosome were not identical. Given the higher complexity found in the genomic organization of the DEFB4 and DEFB104 genes, association studies with CF lung disease severity were performed only for frequent polymorphisms located in DEFB1. No association with the age of first infection by Pseudomonas aeruginosa or with the FEV1 percentage at the age of 11-13 years could be found. © 2005 Elsevier Inc. All rights reserved
The effect of thymic humoral factor upon regeneration of lymphatic and haemopoietic tissues of irradiated mice.
No full text26 Establishment of borderline sweat chloride concentrations: statistical analysis of long-term Czech data
No full textSelf-regulation in children and minors in institutional care
No full textThe study deals with self-regulation in children and minors (aged 11 to 19 years) living in so-called "total institutions". It examines the degree of self-regulation of behaviour from the perspective of the children and minors themselves and from the perspective of their key workers. Children and minors and their key workers differ significantly in perception of the wards' self-regulation of behaviour in the short and long-term context. The lowest rate of self-regulation in children and minors in institutional care is reflected in the area of regulation of emotions. The results point to certain specificity of the institutional care environment. © The author(s)
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