304 research outputs found
J. W. Manak Blacksmith and Woodwork Shop
No full textPhotograph of blacksmith shop. Two men and three children are standing by the doorway in front of the shop. The sign on facade states: "J.W.MANAK, BLACKSMITH & WOODWORK"
Corrosion and Electrochemical Properties of Laser-Shock-Peening-Treated Stainless Steel AISI 304L in VVER Primary Water Environment
No full textLaser Shock Peening (LSP) is a surface treatment technique for metallic materials. It induces plastic deformation at the surface of up to around 1 mm in depth. This process introduces residual stresses that lead to strain hardening, and potentially improvements in fatigue, stress corrosion cracking (SCC) and general corrosion behaviour in many, but not all, corrosive media. In this paper, two specimens made of AISI 304L stainless steel, one LSP-treated and one un-treated, were tested at 280 °C and 8 MPa in VVER (or PWR) primary circuit water chemistry using in situ Electrochemical Impedance Spectroscopy (EIS). This experiment serves to qualify the influence of LSP on the changes in corrosion behaviour in high-temperature, high-density water. The residual stress (RS) measurement of the surface showed a compression RS. Before LSP treatment, RS at the surface was 52.2 MPa in the rolling direction 0°RD and 10.42 MPa in the transverse rolling direction 90°RD. After the treatment, surface RS was −175.27 MPa and −183.51 MPa for Scan and TScan directions, respectively. The effect of compressive RS at the surface was studied and showed an increase in corrosion rate. The analysis of oxide layer by SEM revealed differences between LSP-treated and untreated AISI 304L specimens and their connection to corrosion rates
Technological and institutional approach for enhancing water (logged) productivity in agriculture: a case study of Ganga Basin in Allahabad
No full textWater productivityCase studiesRiver basinsWaterloggingDrainageIrrigation canalsLand useInstitutionsWater users associations
Third annual exhibition, Western Federation of Watercolor Societies Exhibition by the Watercolor Art Society-Houston in conjunction with the Houston Public Library
No full textThe Western Federation of Watercolor Societies is a collective partnership amongst several Watercolor Societies in the Western United States. Every year this federation sponsors an annual juried exhibition of collage and water-based media. The third annual watercolor exhibition was hosted by Watercolor Art Society-Houston in conjunction with the Houston Public Library. Alexander Ross and Lee Weiss juried the show. The First Award winner was Manak for Magdalena by Stevie Kesner. The Second Award winner was Collector\u27s Corner by Robery Landry
Complex epigenomes of endocycling ovaries vs. mitotic testis in the closest living relative of vertebrates
No full text<p><strong>Abstract</strong></p>
<p>The animal epigenome is composed of interconnected distinct functions, where the key is a chromatin structure and dynamics. Epigenetic information is highly specific for certain cell type and developmental stage and dynamically changes with the cell cycle. Here we present thorough analysis of epigenome components of maturing gonads of Oikopleura dioica. Use of ovary and testes samples enabled us to compare between mitotic male germline and endocycling female nurse cells. The urochordate Oikopleura is considered the closest relative to vertebrates with a great proportion of its extremely compact genome being splice-led and/or transcribed in operons. Genome-wide ChIP-chip profiles of numerous posttranslational histone modifications, along with RNA polymerase II, CTCF and p300 binding sites were generated from two homogeneous cell population samples. To capture the combinatorial action of the histone modifications we applied unsupervised learning method and annotated the genome with 15 distinct chromatin states and related those to numerous genomic features. Besides many conserved histone modification functions, we found some exceptions, which could be attributed to sex chromosomes, cell cycle mode or germ line-specificity. Interestingly, the nurse nuclei pattern is in agreement to what is known from other metazoan epigenomes, but testis epigenome revealed quite different histone code. In addition, to gain more insight into the cell cycle and developmental stage specificities of the histone modifications, we analyzed expression profiles of panel of histone modifier enzymes and address the complexity of the chromatin dynamics from this point of view. Overall, we provide a detailed structure of an early chordate epigenome.</p>
<p><strong>References:</strong></p>
<p>Jenuwein, T., & Allis, C. D. (2001). Translating the histone code. Science (New York, N.Y.), 293(5532), 1074–1080Ho, J. W. K., Jung, Y. L., Liu, T., Alver, B. H., Lee, S., Ikegami, K., … Park, P. J. (2014). Comparative analysis of metazoan chromatin organization. Nature, 512(7515), 449–452. doi:10.1038/nature13415Fuchs, J., Demidov, D., Houben, A., & Schubert, I. (2006). Chromosomal histone modification patterns--from conservation to diversity. Trends in Plant Science, 11(4), 199–208Delsuc, F., Brinkmann, H., Chourrout, D., & Philippe, H. (2006). Tunicates and not cephalochordates are the closest living relatives of vertebrates. Nature, 439(7079), 965–8. doi:10.1038/nature04336Denoeud, F., Henriet, S., Mungpakdee, S., Aury, J.-M., Da Silva, C., … Chourrout, D. (2010). Plasticity of animal genome architecture unmasked by rapid evolution of a pelagic tunicate. Science (New York, N.Y.), 330(6009), 1381–5.Bouquet, J.-M., Spriet, E., Troedsson, C., Otterå, H., Chourrout, D., & Thompson, E. M. (2009). Culture optimization for the emergent zooplanktonic model organism Oikopleura dioica. Journal of Plankton Research, 31(4), 359–370.Moosmann, A., Campsteijn, C., Jansen, P. W., Nasrallah, C., Raasholm, M., Stunnenberg, H. G., & Thompson, E. M. (2011). Histone variant innovation in a rapidly evolving chordate lineage. BMC Evolutionary Biology, 11(1), 208.Danks, G., Campsteijn, C., Parida, M., Butcher, S.,… Manak, J. R. (2013). OikoBase: a genomics and developmental transcriptomics resource for the urochordate Oikopleura dioica. Nucleic Acids Research, 41, D845–53.Ganot, P., Bouquet, J.-M., Kallesøe, T., & Thompson, E. M. (2007). The Oikopleura coenocyst, a unique chordate germ cell permitting rapid, extensive modulation of oocyte production. Developmental Biology, 302(2), 591–600.Ernst, J., & Kellis, M. (2012). ChromHMM: automating chromatin-state discovery and characterization. Nature Methods, 9(3), 215–6.</p>
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Benign-Ex: Delineating Regions of the Human Genome Benign to Copy Number Variation. Alyssa S. Wetzel, Heather Major, Mrutyunjaya Parida, J. Robert Manak, Benjamin W. Darbro. medRxiv 2022.10.17.22280252; doi: https://doi.org/10.1101/2022.10.17.22280252
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Industrial Location in India under Liberalization
No full textThe economic liberalization policy initiated in the country since 1991 has made large-scale delicensing of industry and changes in the industrial location policies along with the stabilization-cum-structural adjustments of the economy. This curtailed the role of the state as industrial owner and location regulator and increases the role of private sector in industrialization. With the increasing dominance of private sector in industrialization under the liberalization policy it is expected that industries will be more spatially concentrated in the leading industrial regions. However, the neoclassical principle suggests that in the long run “divergence is followed by convergence”. This is in contrast with the theory that raises the question about the regional industrial development in India under the two policy regimes (an inward looking restrictive policy regime prior to 1980s and liberalization policy since 1991). The main objective of our study is to see whether there is convergence or divergence of industrial location and also the relative concentration of industries within the states in the post liberalization period, and thus, understands the influence of economic liberalization on industrial location in India. These two objectives are examined with the employment data of organized manufacturing sector for the pre- and post-reform periods using: first coefficient of variation of manufacturing employment, aggregated for all industries and second, location quotients and specialization coefficients, disaggregated into three use-based manufacturing sectors (consumer goods, intermediate goods and capital goods). Our study finds that there is more concentration of the manufacturing industries in the post liberalization period in India and the tendency to catch up the industrially developed states is hardly seen among the backward states, which suggest widening inter-regional divergence, as against the neo-classical principle “divergence followed by convergence”.Industrial Location, Economic Liberalization, India
Seizures are regulated by ubiquitin-specific peptidase 9 x-linked (USP9X), a de-ubiquitinase
No full textEpilepsy is a common disabling disease with complex, multifactorial genetic and environmental etiology. The small fraction of epilepsies subject to Mendelian inheritance offers key insight into epilepsy disease mechanisms; and pathologies brought on by mutations in a single gene can point the way to generalizable therapeutic strategies. Mutations in the PRICKLE genes can cause seizures in humans, zebrafish, mice, and flies, suggesting the seizure-suppression pathway is evolutionarily conserved. This pathway has never been targeted for novel anti-seizure treatments. Here, the mammalian PRICKLE-interactome was defined, identifying prickle-interacting proteins that localize to synapses and a novel interacting partner, USP9X, a substrate-specific de-ubiquitinase. PRICKLE and USP9X interact through their carboxy-termini; and USP9X de-ubiquitinates PRICKLE, protecting it from proteasomal degradation. In forebrain neurons of mice, USP9X deficiency reduced levels of Prickle2 protein. Genetic analysis suggests the same pathway regulates Prickle-mediated seizures. The seizure phenotype was suppressed in prickle mutant flies by the small-molecule USP9X inhibitor, Degrasyn/WP1130, or by reducing the dose of fat facets a USP9X orthologue. USP9X mutations were identified by resequencing a cohort of patients with epileptic encephalopathy, one patient harbored a de novo missense mutation and another a novel coding mutation. Both USP9X variants were outside the PRICKLE-interacting domain. These findings demonstrate that USP9X inhibition can suppress prickle-mediated seizure activity, and that USP9X variants may predispose to seizures. These studies point to a new target for anti-seizure therapy and illustrate the translational power of studying diseases in species across the evolutionary spectrum.Lily Paemka, Vinit B. Mahajan, Salleh N. Ehaideb, Jessica M. Skeie, Men Chee Tan, Shu Wu, Allison J. Cox, Levi P. Sowers, Jozef Gecz, Lachlan Jolly, Polly J. Ferguson, Benjamin Darbro, Amy Schneider, Ingrid E. Scheffer, Gemma L. Carvill, Heather C. Mefford, Hatem El-Shanti, Stephen A. Wood, J. Robert Manak, Alexander G. Bassu
Generalized schematic for dialect learning.
No full text(A) Female flies cannot efficiently communicate a wasp threat if “teacher” and “student” are from different diverged species. (B) Cohabitation of diverged species allows for each species to learn the language “dialects” of the others; once dialects are learned, a female teacher now can communicate a wasp threat to a female student from a diverged species. Different diverged Drosophila species are highlighted in different colors (orange, green, blue, brown). Wasp is highlighted in black.</p
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