89 research outputs found
Correlation of phytochemical content with antioxidant potential of various sweet potato (Ipomoea batatas) in West Java, Indonesia
No full textObjective: To determine antioxidant activity and phytochemical content from various tubers extracts of sweet potato (Ipomoea batatas) and to explore the correlation of phytochemical content with their antioxidant activities. Methods: Antioxidant activities were tested using DPPH and FRAP assays. Total phenolic was calculated by Folin-Ciocalteu reagent, flavonoid content by Chang’s method and correlation with their antioxidant activities were analyzed by Pearson’s method. Results: PO2 showed highest antioxidant activity, which had the lowest IC50 DPPH (10.54 μg/mL) and the lowest EC50 FRAP (11.14 μg/mL). PO2 showed the highest total phenolic (11.91 g GAE/100 g) and total flavonoid content (17.83 g QE/100 g). There were significantly negative correlation between total phenolic content and flavonoid content in sample PO with their IC50 DPPH and EC50 FRAP. IC50 DPPH of sample PP and PO showed significantly positive correlation with their EC50 FRAP. Conclusions: Result of DPPH method shows that all different ethyl acetate and ethanolic tubers extracts of four varieties of sweet potato are classified as strong and very strong antioxidant. Result of DPPH and FRAP methods indicates that phenolic and flavonoid compounds in sample PO contributes together to antioxidant activities. Antioxidant activities of sample PP and PO by DPPH method are linear to FRAP method
ANTIOXIDANT ACTIVITIES OF ARABICA GREEN COFFEE FROM THREE REGIONS USING ABTS AND DPPH ASSAYS
No full textABSTRACTObjectives: The aim of this research were to determine antioxidant activity from various extracts of arabica green coffee from three different regionsusing two methods of antioxidant testing which were 2,2\u27-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl(DPPH) and correlation of total phenolic, flavonoid, and carotenoid content in various extracts of arabica coffee with their IC50 of ABTS and IC50 ofDPPH antioxidant activities.Methods: Extraction was conducted by reflux using various polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidantactivities using ABTS and DPPH assays, determination of total phenolic, flavonoid, and carotenoid content were performed by UV-visiblespectrophotometry and its correlation with IC50 of ABTS and IC50 of DPPH scavenging activities were analyzed by Pearson\u27s method.Results: The lowest IC50 of ABTS scavenging activity 3.47 µg/ml was given by n-hexane extract of Toraja arabica coffee, while ethanolic extract ofLintong arabica coffee gave the lowest IC50 of DPPH scavenging activity 0.7 µg/ml. Ethanolic extract of Madailing arabica coffee gave the highestphenolic content, and its ethyl acetate extract had the highest total flavonoid. There were negative and high correlation between phenolic content andcarotenoid content in Lintong arabica coffee extract with their IC50 of DPPH.Conclusions: All of the arabica green coffee extracts from three regions except LIN1 by ABTS method were categorized as a very strong antioxidant andexcept TOR1 for DPPH method. Phenolic and carotenoid compounds in Lintong arabica coffee extracts were the major contributor in IC50 of DPPHscavenging activities. Arabica coffee extracts from Lintong showed the linear result in ABTS and DPPH assays.Keywords: Antioxidant, 2,2\u27-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl, Arabica green coffee, Phenolic,Flavonoid, Carotenoid
EVALUATION OF ANTIOXIDANT ACTIVITIES OF FRUIT EXTRACTS OF CHAYOTE (SECHIUM EDULE [JACQ.] SWARTZ) GROWN IN DIFFERENT SITES IN JAVA - INDONESIA
No full textABSTRACTObjectives: The aim of this research were to determine antioxidant activity from various fruit extracts of chayote from three different sites using twoantioxidant methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and phosphomolybdenum methods, correlation of total phenolic, flavonoid,and carotenoid content in various extracts of chayote with their IC50 of DPPH antioxidant activities and EC50 of phosphomolybdenum capacity.Methods: An extraction was carried out by reflux using various polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidantactivities using DPPH and phosphomolybdenum assays, determination of total phenolic, flavonoid, and carotenoid content were conducted byultraviolet-visible spectrophotometry and its correlation with ICResults: The lowest IC5050 of DPPH and EC50 of phosphomolybdenum were analyzed by Pearson\u27s method. of DPPH scavenging activity was given by n-hexane fruit extract of chayote from Lembang (9.32 µg/ml), while the lowest ECof phosphomolybdenum capacity was given by ethyl acetate fruit extract of chayote from Semarang (209.87 µg/ml). Ethyl acetate chayote fruit extractfrom Malang gave the highest phenolic content and its n-hexane extract had the highest total flavonoid. There were negative and significant correlationbetween total flavonoid content in all of the chayote fruit extracts from three different sites with their IC50 of DPPH and ECConclusions: N-hexane chayote fruit extract from Lembang and Semarang and ethyl acetate chayote fruit extract from Semarang were categorizedas a very strong antioxidant by DPPH method. Flavonoid compounds in all of the chayote fruit extracts from three different locations were the majorcontributor in their antioxidant activities by DPPH and phosphomolybdenum methods. All of the chayote fruit extracts from Lembang, Semarang, andMalang had a linear result in DPPH and phosphomolybdenum assays.Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Phosphomolybdenum, Chayote, Fruit, Different sites.50of phosphomolybdenum.50Â
ANTIOXIDANT CAPACITIES FROM DIFFERENT POLARITIES EXTRACTSOF THREE KINDS GINGERUSINGDPPH, FRAPASSAYS ANDCORRELATION WITH PHENOLIC, FLAVONOID, CAROTENOID CONTENT
No full textObjectives: The objectives of this research were to study antioxidant capacityfrom different polarities extracts of three kinds ginger using two methods of antioxidant testing which were DPPH (2.2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) and correlation of total phenolic, flavonoid and carotenoid content in different polarities extracts of three kinds gingerwith DPPH and FRAP antioxidant capacities.
Methods: Extraction was performed by reflux using different polarities solvents. The extracts were vaporated using rota vapor. Then antioxidant capacities were tested using DPPH and FRAP assays. Determination of total phenolic, flavonoid and carotenoid content were performed by spectrophotometry UV-Vis and its correlation with FRAP and DPPH antioxidant capacities were analyzed by Pearson method.
Results: EG3(ethanol extract of elephant ginger rhizomes) had the highest DPPH scavenging capacity with IC50 0.26 ppmandEG3had the highest FRAP capacity also with EC5091.90 ppm.SG1(n-hexane extract of small ginger) contained the highest total phenolic (14.56 g GAE/100 g), EG2(ethyl acetate extract of elephant ginger rhizomes) had highest flavonoid content (7.5 gQE/100 g) andEG3 had the highest carotenoid 0.95 g BET/100 g.
Conclusions: There were positively high correlation between total phenolic content in small ginger rhizomes extracts with their antioxidant activity using DPPH and FRAP assays. DPPH scavenging capacities in small ginger rhizomes extracts had positively high correlation with their FRAP capacities
ANTIOXIDANT EVALUATION AND PHYTOCHEMICAL CONTENT OF VARIOUS RICE BRAN EXTRACTS OF THREE VARIETIES RICE FROM SEMARANG-CENTRAL JAVA, INDONESIA
No full textObjectives: The objectives of this research were to evaluate antioxidant activity from different polarities rice bran extract of three varieties of rice using two methods of antioxidant testing which were FRAP (Ferric Reducing Antioxidant Power) and DPPH (2,2-diphenyl-1-picrylhydrazyl), and correlation of total phenolic, flavonoid and carotenoid content with their EC50 of FRAP and IC50 of DPPH antioxidant activities. Methods: Extraction was conducted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Determination of total phenolic, flavonoid and carotenoid content, antioxidant activities using FRAP and DPPH assays were performed by UV-visible spectrophotometry and its correlation with EC50 of FRAP capacities and IC50 of DPPH scavenging activities were analyzed by Pearson\u27s method. Results: Ethanolic rice bran extract of black rice showed the lowest EC50 of FRAP capacity 64.35 µg/ml and IC50 of DPPH scavenging activity 23.92 µg/ml. The highest phenolic content, flavonoid content and carotenoid content were also given by ethanolic rice bran extract of black rice. There were significantly negative correlation between total phenolic content and carotenoid content in rice bran extract of red rice and black rice with their IC50 of DPPH. Conclusions: All of rice bran extracts (except n-hexane rice bran extract of black rice and ethanolic rice bran extract of white rice) were very strong antioxidant, by DPPH assay. Phenolic and carotenoid compounds in rice bran extracts of red rice and black rice were the major contributor in antioxidant activity by DPPH assay. Rice bran extracts of black rice had linear results by FRAP and DPPH assays.Â
ANTIOXIDANT ACTIVITIES OF DIFFERENT POLARITY EXTRACTS FROM THREE ORGANS OF MAKRUT LIME (CITRUS HYSTRIX DC) AND CORRELATION WITH TOTAL FLAVONOID, PHENOLIC, CAROTENOID CONTENT
No full textABSTRACTObjectives: The objectives of this research were to study antioxidant activities from various extracts of three organs of makrut lime (Citrus hystrix)using two methods of antioxidant assays, which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and cupric ion reducing antioxidant capacity (CUPRAC);and correlation of total flavonoid, phenolic, and carotenoid content in various extracts of three organs of makrut lime with IC50 of DPPH antioxidantactivities and EC50 of CUPRAC capacities.Methods: Extraction was performed by reflux apparatus using different polarity solvents. The extracts were evaporated using rotary evaporator.Antioxidant capacities were tested using DPPH and CUPRAC assays. Determination of total phenolic, flavonoid and carotenoid content performed byultraviolet-visible and their correlation with IC50 of DPPH scavenging activities and EC50 of CUPRAC capacities were analyzed by Pearson\u27s method.Results: Ethyl acetate stem extract of makrut lime (ST2) had the lowest IC50 of DPPH scavenging activity 0.6 μg/ml and the lowest EC50 of CUPRACcapacity 123 μg/ml. N-hexane stem extract of makrut lime (ST1) had the highest total flavonoid content (8.7 g QE/100 g), ethyl acetate stem extract(ST2) contained the highest total phenolic content (TPC) (8.3 g gallic acid equivalent /100 g and total carotenoid content (1.8 g BE/100 g).Conclusions: There was negatively high correlation between TPC in peel and stem extracts of makrut lime with their IC50 of DPPH scavenging activity.EC50 of CUPRAC capacity of leaves, peel and stem extracts of makrut lime had negative and high correlation with their total flavonoid and carotenoidcontent. IC50 of DPPH scavenging activities in leaves, peel and stem extracts of makrut lime had no linear result with EC50 of CUPRAC capacities.Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Cupric ion reducing antioxidant capacity, Organs, Makrut lime, Flavonoid, Phenolic, Carotenoid
PHYTOCHEMICAL CONTENT AND ANTIOXIDANT POTENTIAL OF DIFFERENT ORGANS OF EGGPLANT (SOLANUM MELONGENA L.) GROWN IN WEST JAVA-INDONESIA
No full text Objectives: The goals of this research were to evaluate antioxidant potential from different organs of eggplant (Solanum melongena L.) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) and correlation of total phenolic and flavonoid content with their inhibitory concentration 50% (IC50) of DPPH, and exhibitory concentration 50% (EC50) of FRAP.Materials and Methods: Each sample was extracted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities were tested using DPPH and FRAP assays, determination of total phenolic and flavonoid content were carried out by ultraviolet-visible spectrophotometry and correlation with their IC50 of DPPH and EC50 of FRAP capacities were analyzed by Pearson’s method.Results: The lowest IC50 of DPPH scavenging activity 1.14 μg/ml and the lowest EC50 of FRAP capacity 49.80 μg/ml was given by ethanolic leaves extract of eggplant. Ethanolic leaves extract of eggplant also presented the highest total phenolic content (TPC) (8.87 g gallic acid equivalent/100 g), while the highest total flavonoid content was shown by ethyl acetate leaves extract (24.50 g quercetin equivalent/100 g). There was a significantly negative correlation between TPC in leaves and fruit extracts of eggplant with their IC50 of DPPH and EC50 of FRAP.Conclusions: All different extracts of eggplant organs (except n-hexane stem extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in eggplant leaves and fruit extracts were the major contributor in antioxidant activities by DPPH and FRAP methods. DPPH and FRAP showed linear results in antioxidant activities of eggplant leaves, fruit and stem extracts.</jats:p
ANTIOXIDANT PROFILE AND PHYTOCHEMICAL CONTENT OF THREE KINDS OF LEMON GRASS GROWN IN WEST JAVA-INDONESIA
No full textABSTRACTObjective: The aims of this research were to determine antioxidant activity from various herbs extracts of three kinds of lemon grass using twoantioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) and correlation of totalphenolic content (TPC), total flavonoid content (TFC), and total carotenoid contents (TCC) with their inhibitory concentration 50% (IC) of DPPH andexhibitory concentration 50% (EC50) of FRAP.Methods: A sample was extracted by reflux method using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidantactivities using DPPH and FRAP assays, determination of TPC, TFC, and TCC were carried out by ultraviolet -visible spectrophotometry and correlationwith their IC50 of DPPH and EC50 of FRAP capacities were analyzed by Pearson\u27s method.Results: The ethanolic herbs extract of Cymbopogon citratus (CC) had the lowest IC5050 of DPPH scavenging activity 2.75 µg/ml and the lowest ECof FRAP capacity 12.22 µg/ml. Ethanolic herbs extract of Cymbopogon winterianus exposed the highest phenolic content and its n-hexane extractpresented the highest carotenoid content. Ethyl acetate herbs extract of CC gave the highest flavonoid content. There was significantly negativecorrelation between TPC in CC herbs extract with their IC50 of DPPH and EC50 of FRAP.Conclusions: All herbs extracts from three kinds of lemon grass were categorized as a very strong antioxidant by DPPH method. Phenolic compoundsin CC were the major contributor in antioxidant activities by DPPH and FRAP methods. DPPH and FRAP gave linear result in antioxidant activities ofherbs extract of three kinds of lemon grass.Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ferric reducing antioxidant power, Lemon grass, Three kinds, Herbs.5
Phytochemical compounds and pharmacological activities of Ipomoea batatas L.: An updated review
No full textThis article provides an overview of Ipomoea batatas L., the Ipomoea genus. The review covers traditional uses, nutritional value, phytochemical compounds, pharmacological activities, and toxicity studies. Data were collected from scientific databases and search engines. Sweet potatoes are used in various countries for traditional uses such as dietary fiber sources, treating allergies, and providing energy in diabetes mellitus treatment. The primary phytochemical compounds in Ipomoea batatas are phenolic compounds, flavonoids, anthocyanins, and carotenoids. Sweet potato contains several nutritional constituents: vitamin C, protein, fiber, carbohydrates, β-carotene, and minerals. Sweet potato exhibits various pharmacological activities, such as antioxidant, aphrodisiac, anticancer, and anti-inflammatory. The types of phytochemical compounds in each part of the plant are different. Each pharmacological activity and mechanism of action depends on the phytochemical compounds, part and variety of the plant, and extraction solvent. However, further study is required to investigate the chronic toxicity of Ipomoea batatas
DIURETIC ACTIVITY OF MATOA LEAVES EXTRACTS (Pometiapinnata) AND ITS INFLUENCE ON POTASSIUM AND SODIUM LEVELS
No full textObjective: The purpose of this study was to determine diuretic activity of matoa leaves (Pometiapinnata) extracts and fraction and its influence on potassium and sodium levels.Methods: Matoa leaves were extracted by reflux method followed by evaporation using rotary evaporator. The subjects were male Wistar rats that were divided into 11 group furosemide (3.6 mg/kg bw), control group CMC 0.5%, matoa leaves extracts with doses of 50 mg/kg bw, 100 mg/kg bw, 150 mg/kg bw, matoa leaves aqueous fraction with dose of 10.94 mg/kg bw, 21.88 mg/kg bw, 32.82 mg/kg bw, matoa leaves ethyl acetate fraction with dose of 4.35 mg/kg bw, 8.71 mg/kg bw, 13.06 mg/kg bw. Rats were placed in metabolic cagesduring observation study. Urine volume was measured for 5 to 24 hours. Potassium and sodium levels in urine were determined by using Atomic Absorption Spectrophotometry.Results: The effective dose of matoa leaves extract and fractions for diuretic activity was matoaleaves ethyl acetate fraction 8.71 mg/kg bw which could increase the excretion of sodium and potassium in the urine of the male Wistar rats.Conclusion:Matoa leaves extract and fractions could increase the excretion of sodium and potassium in the urine of the male Wistar rats. </jats:p
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