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LON-2 peptides recovered from the IP-MS experiments.
LON-2 peptides recovered from the IP-MS experiments.</p
IP-MS approach from mouse skin to identify Vangl2 PTMs.
IP-MS approach from mouse skin to identify Vangl2 PTMs.</p
Target of AVRcap1b identified via IP-MS.
(A) Immune affinity enriched protein abundance plot generated based on proteins groups identified for AVRcap1b and PexRD54. Protein abundances were calculated as the total mean spectral counts identified with each effector across 3 replicates. The Log2 of the ratio of mean counts observed for AVRcap1b divided by the mean counts observed for PexRD54 was calculated. All 261 proteins identified were then sorted from highest to lowest ratio and plotted in this order (see S14 Table for details). Proteins with the highest ratio correspond to the most likely AVRcap1b (blue) specific interactors. Proteins most likely to correspond to PexRD54 are labelled in green. Proteins in grey were identified in both effectors. (B) Eight putative unique AVRcap1b proteins (based on previously mentioned ratio of mean spectral counts) identified in IP-MS experiment. Unique spectral counts obtained in each of the 3 independent replicates with AVRcap1b are included on the right-hand side. IP-MS, immunoprecipitation–mass spetrometry. (TIF)</p
Colorectal cancer anti-ORF1p LFQ IP-MS
Here, three colorectal tumors were subjected to anti-ORF1p LFQ, IP-MS: (A) Krukenberg Carcinoma, Ovary; (B) Metastatic Rectal Adenocarcinoma, Liver; (C) Adenocarcinoma, Colon. These were accompanied by matched normal IP controls and/or mouse IgG IP controls. The objective is to map LINE-1 RNP interactions in cancer
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