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Post-implantation embryo development in the South American plains vizcacha, Lagostomus maximus (Rodentia, Caviomorpha)
No full textThe early embryo development after implantation varies among mammals. In muroid rodents, an egg cylinder is formed by folding during gastrulation; however, in most mammals, a fat disc develops after the embryo implants in the uterine wall. In contrast to what occurs in muroids, the embryo of the caviomorph Lagostomus maximus develops from a bilaminar disc by cavitation rather than folding. The fat disc morphology of the epiblast is shared with non-rodent mammals such as rabbits, pigs, cows, and humans and is considered the mammotypic embryonic morphology. Nevertheless, the fat embryo of L. maximus is a somewhat intermediate situation between the mammotypical bilaminar disc and the egg cylinder of muroids. In muroid rodents, the extraembryonic ectoderm is in close contact with the epiblast as a consequence of embryo folding during gastrulation. In contrast, the typical fat disc of mammals does not show extraembryonic ectoderm. L. maximus embryo develops extraembryonic ectoderm homologous to that found in muroids; however, it remains far apart from the epiblast by the visceral endoderm. Here we describe the embryonic and fetal development throughout gestation in L. maximus by discussing similarities and differences with other mammals from an evolutionary and phylogenetic perspective.Fil: Leopardo, Noelia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Inserra, Pablo Ignacio Felipe. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
Embryo development in the South American plains vizcacha, Lagostomus maximus (Rodentia, Caviomorpha)
No full textThe early embryo development after implantation varies among mammals. In muroid rodents, an egg cylinder is formed by folding during gastrulation; however, in most mammals, a fat disc develops after the embryo implants in the uterine wall. In contrast to what occurs in muroids, the embryo of the caviomorph Lagostomus maximus develops from a bilaminar disc by cavitation rather than folding. The fat disc morphology of the epiblast is shared with non-rodent mammals such as rabbits, pigs, cows, and humans and is considered the mammotypic embryonic morphology. Nevertheless, the fat embryo of L. maximus is a somewhat intermediate situation between the mammotypical bilaminar disc and the egg cylinder of muroids. In muroid rodents, the extraembryonic ectoderm is in close contact with the epiblast as a consequence of embryo folding during gastrulation. In contrast, the typical fat disc of mammals does not show extraembryonic ectoderm. L. maximus embryo develops extraembryonic ectoderm homologous to that found in muroids; however, it remains far apart from the epiblast by the visceral endoderm. Here we describe the embryonic and fetal development throughout gestation in L. maximus by discussing similarities and differences with other mammals from an evolutionary and phylogenetic perspective.Fil: Leopardo, Noelia Paola. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Inserra, Pablo Ignacio Felipe. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
Genetic variation across Lagostomus maximus geographical distribution in Argentina
No full textStudying the amount and pattern of genetic diversity within and between populations is one of the central aims of population genetics. However, the population genetic structure of many species, including Lagostomus maximus, is currently poorly understood. The analysis of the mitochondrial DNA control region reveals low levels of genetic variation in L. maximus populations throughout argentina. Despite the recognition of three morphologically distinct subspecies in Argentina, and the species’ tendency for philopatry among females and the average home range of the species of 1.3 ha, a single widely distributed population inhabits Argentina. Metapopulation processes and changes in human population dynamics in the late Holocene may have played a role in shaping the population genetic structure of L. maximus in Argentina.Fil: Gariboldi, María Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Inserra, Pablo Ignacio Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Tunez, Juan Ignacio. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
The South American Plains Vizcacha, Lagostomus maximus, as a Valuable Animal Model for Reproductive Studies
Get PDFThe vast majority of our understanding of the mammalianreproductive biology comes from investigations mainlyperformed in mice, rats and humans. However, evidencegathered from non-conventional laboratory models, farm andwild animals strongly suggests that reproductive mechanismsshow a plethora of different strategies among species. Forinstance, studies developed in unconventional rodents suchas guinea pigs and hamsters, that share with humans someendocrine and reproductive characters, have contributed to abetter understanding of human physiology and disease [1,2]. Abetter knowledge on the variety of mechanisms that regulatereproduction could lead to improve early diagnosis, treatment,or novel strategies development to ameliorate fertility andguarantee a successful reproduction. In this letter, we brieflyintroduce Lagostomus maximus, an unconventional rodentwhose neuro-endocrinology and reproduction in general haveattracted significant interest in recent years in view of its unusualreproductive traits.Fil: Dorfman, Verónica Berta. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Inserra, Pablo Ignacio Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Leopardo, Noelia Paola. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Halperin, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Vitullo, Alfredo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentin
The world pandemic of vitamin D deficit culd possibly be explained by cellular inflammatory response activity induced by the renin angiotensin system
Get PDFThis review attempts to show that there may be a relationship between inflammatory processes induced by chronic overstimulation of the renin-angiotensin system (RAS) and the worldwide deficiency of vitamin D (VitD) and that both disorders are probably associated with environmental factors. Low VitD levels represent a risk factor for several apparently different diseases, such as infectious, autoimmune, neurodegenerative, and cardiovascular diseases, as well as diabetes, osteoporosis, and cancer. Moreover, VitD insufficiency seems to predispose to hypertension, metabolic syndrome, left ventricular hypertrophy, heart failure, and chronic vascular inflammation. On the other hand, inappropriate stimulation of the RAS has also been associated with the pathogenesis of hypertension, heart attack, stroke, and hypertrophy of the left ventricle and vascular smooth muscle cells. Because VitD receptors (VDRs) and RAS receptors are almost distributed in the same tissues, a possible link between VitD and the RAS is even more plausible. Furthermore, from an evolutionary point of view, both systems were developed simultaneously, actively participating in the regulation of inflammatory and immunological mechanisms. Changes in RAS activity and activation of the VDR seem to be inversely related; thus any changes in one of these systems would have a completely opposite effect on the other, making it possible to speculate that the two systems could have a feedback relationship. In fact, the pandemic of VitD deficiency could be the other face of increased RAS activity, which probably causes lower activity or lower levels of VitD. Finally, from a therapeutic point of view, the combination of RAS blockade and VDR stimulation appears to be more effective than either RAS blockade or VDR stimulation individually.Fil: Ferder, Marcelo. Universidad de Buenos Aires. Facultad de Medicina; Argentina;Fil: Inserra, Pablo Ignacio Felipe. Universidad Austral. Facultad de Cs.biomedicas; Argentina;Fil: Manucha, Walter Ariel Fernando. Universidad Nacional de Cuyo; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina;Fil: Ferder, León. Ponce School of Medicine and Health Sciences; Puerto Rico
Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserve
Get PDFFemale germ line in mammals is subjected to massive cell death that eliminates 60-85% of the germinal reserve by birth, and continues from birth to adult life until the exhaustion of the germinal pool. Germ cell demise occurs mainly through apoptosis by means of a biased expression in favour of pro-apoptotic members of the BCL2 gene family. In contrast, the South American plains vizcacha, Lagostomus maximus, shows as sustained expression of the anti-apoptotic BCL2 gene throughout gestation and a low incidence of apoptotic germ cells. This led to propose that, in the absence of death mechanisms other than apoptosis, female germ line should continuously increase from foetal life until after birth. We quantified all healthy germ cells and follicles in L. maximus ovaries from early foetal life to day 60 after birth using unbiased stereological methods and labelling with TUNEL assay to detect apoptosis. Healthy germ cell population increased continuously from early developing ovary reaching a 50 times higher population number by the end of gestation. TUNEL positive germ cells were <0.5% of germ cell number except at mid-gestation (3.62%). Mitotic proliferation, entrance to prophase I and primordial follicle formation occurred as overlapped processes from early pregnancy to birth. Germ cell number remained constant in early postnatal life but a remnant population of non-follicular VASA and PCNA positive germ cells still persisted at postnatal day 60. L. maximus is the first mammal so far described in which female germ line develops in the absence of constitutive massive germ cell elimination.Fil: Inserra, Pablo Ignacio Felipe. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leopardo, Noelia Paola. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Willis, Miguel Alfredo. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Freysselinard, A. L.. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
Reproductive neuroendocrinology of the female South American plains vizcacha, Lagostomus maximus
No full textFemale plains vizcachas have several peculiar reproductive characteristics as a true reactivation of the hypothalamic–pituitary–ovarian axis during pregnancy among others. This axis-reactivation is enabled by precise regulation of the hypothalamic gonadotropin-releasing hormone (GnRH) neurons allowing the pulsatile delivery of GnRH at mid-gestation. Such an event is followed by sequential secretion of estradiol (E2), follicle-stimulating hormone (FSH), and luteinizing hormone (LH), ultimately leading to a reboot of luteal steroidogenesis and a consequent boost of progesterone (P4) that enables the successful delivery of (at most) two well-developed newborns. The regulation of the hypothalamic GnRH neurons results from the combination of different endocrine pathways. GnRH neurons located in the preoptic area express progesterone and estrogens receptors, and aromatase, the estradiol-synthesizing enzyme. This local expression points to a mechanism of direct hormonal regulation of GnRH neurons that would synergize with the classic feedback pathways driven from the ovaries. Furthermore, the conspicuous organization of the kisspeptin system and the neuromodulators prolactin and dopamine observed in this area strongly suggests their involvement in the modulation of GnRH neurons. All these hormones, receptors, enzymes, and neurotransmitters coordinately regulate the functioning of this distinctive mechanism of which many details remain to be elucidated. In addition, photoperiod information transduced through melatonin hormone would coordinate plains vizcacha gestation with the most favorable moment for offspring surviving. To our knowledge, plains vizcacha is the unique rodent species that has a true reactivation of the reproductive axis during pregnancy. This trait is likely related to a reproductive strategy of plains vizcacha favoring offspring success.Fil: Dorfman, Verónica Berta. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Inserra, Pablo Ignacio Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Vitullo, Alfredo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Halperin, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentin
Lungs as Target of COVID-19 Infection: Protective Common Molecular Mechanisms of Vitamin D and Melatonin as a New Potential Synergistic Treatment
Get PDFCOVID-19 pandemic has a high mortality rate and is affecting practically the entire world population. The leading cause of death is severe acute respiratory syndrome as a consequence of exacerbated inflammatory response accompanied by uncontrolled oxidative stress as well as the inflammatory reaction at the lung level. Until now, there is not a specific and definitive treatment for this pathology that worries the world population, especially the older adults who constitute the main risk group. In this context, it results in a particular interest in the evaluation of the efficacy of existing pharmacological agents that may be used for overcoming or attenuating the severity of this pulmonary complication that has ended the lives of many people worldwide. Vitamin D and melatonin could be good options for achieving this aim, taking into account that they have many shared underlying mechanisms that are able to modulate and control the immune adequately and oxidative response against COVID-19 infection, possibly even through a synergistic interaction. The renin-angiotensin system exaltation with consequent inflammatory response has a leading role in the physiopathology of COVID-19 infection; and it may be down-regulated by vitamin D and melatonin in many organs. Therefore, it is also essential to analyze this potential therapeutic association and their relation with RAS as part of this new approach.Fil: Martín Giménez, Virna Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Catolica de Cuyo. Facultad de Ciencias Quimicas y Tecnologicas; ArgentinaFil: Inserra, Pablo Ignacio Felipe. Universidad de Maimónides; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tajer, Carlos Daniel. Hospital El Cruce Néstor Kirchner; ArgentinaFil: Mariani, Javier. Hospital El Cruce Néstor Kirchner; ArgentinaFil: Ferder, Leon Fernando. Universidad de Maimónides; ArgentinaFil: Reiter, Russel. University Of Texas At San Antonio.; Estados UnidosFil: Manucha, Walter Ariel Fernando. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentin
Actividad del eje hipotálamo-hipófisis-gonadal en la vizcacha de las llanuras de sudamérica (lagostomus maximus) durante la gestación
No full textEl eje Hipotálamo-Hipófisis-Gonadal (HHG) controla el funcionamiento ovárico a través de la Hormona Liberadora de Gonadotrofinas (GnRH) y las hormonas gonadotróficas Hormona Luteinizante (LH) y Hormona Folículo Estimulante (FSH). Posteriormente a la ovulación, y durante la preñez, los esteroides ováricos, Estradiol (E₂) y Progesterona (Pg), ejercen retroalimentación negativa sobre el hipotálamo y la hipófisis.Fil: Inserra, Pablo Ignacio Felipe. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Charif, Santiago Elías. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Schmidt, Alejandro Raúl. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Di Giorgio, Noelia Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Lux, Victoria Adela R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Dorfman, Verónica Berta. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaLVIII Reunión Científica Anual Sociedad Argentina de Investigación Clínica; Reunión Científica Anual 2013 Sociedad Argentina de Fisiología; XLV Reunion Científica Anual Sociedad Argentina de Farmacología ExperimentalMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de FisiologíaSociedad Argentina de Farmacología Experimenta
NF-κB and chemokine-cytokine expression in renal tubulointerstitium in experimental hyperoxaluria. Role of the renin-angiotensin system
No full textRecent evidence indicates that the reninangiotensin system (RAS) seems to play a considerable role in the development of tubulointerstitial (TI) lesions caused by hyperoxaluria (Hox). The purpose of the present study was to evaluate the specific mechanism by which Hox involving RAS induces chemokine and cytokine expression and, therefore, renal TI damage in the ethylene-glycol (ETG) induced hyperoxaluric rat model. Sprague-Dawley rats, separated into five groups, received: G1 regular water, and G2, G3, G4 and G5 1% ETG (a precursor for oxalates) in their drinking water for 4 weeks. An angiotensin converting enzyme inhibitor, benazepril (BZ) 10 mg/kg/day, angiotensin II receptor antagonists, subtype 1 (AT1) losartan (LOS) 40 mg/kg/day and subtype 2 (AT2) PD 123,319 (PD) 10 mg/kg/day, were administered daily to G3, G4 and G5, respectively. At the end of the study, the inflammatory response to Hox was evaluated using antiNF-jB (p50), anti-IL-6, anti-MCP-1; anti-RANTES and anti-ED1 (monocytes/macrophages) in each group. In spite of the same urine oxalate levels, rats belonging to the hyperoxaluric groups treated with either BZ or LOS showed significantly (P<0.01) less TI lesions together with a lower immunoexpression of inflammatory mediators when compared with untreated hyperoxaluric animals. NF-jB (p50) was increased in tubular cells in the ETG group (43.6±8.7 positive cells/mm2 ) and was significantly (P<0.01) reduced by LOS (11.2±4 positive cells/mm2 ) and even more by BZ (6.1±2.4 positive cells/ mm2 ). There was a significant (P<0.01) correlation between NF-jB (p50) positive cells and ED1 cells in the ETG group (r=0.88) and in the ETG+LOS group (r=0.92). LOS showed better control on IL-6 and MCP1 with respect to untreated rats, while BZ showed the best control on RANTES and ED1 cells in comparison with untreated animals. Renal function was significantly (P<0.01) better preserved in BZ and LOS treated groups compared to both untreated animals and rats with PD, as indicated by creatinine clearance values. These results suggest that Hox stimulates the NF-jB cascade and, therefore, induces the overexpression of inflammatory mediators like IL-6, MCP-1, and RANTES. This pathway seems to be mediated not only by AT1 but also by AT2 receptors of angiotensin II.Fil: Toblli, Jorge Eduardo. Hospital Alemán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cao, Gabriel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Alemán; ArgentinaFil: Casas, Gabriel. Hospital Alemán; ArgentinaFil: Stella, Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Inserra, Pablo Ignacio Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Angerosa, Margarita. Hospital Alemán; Argentin
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