30 research outputs found

    Balding Goes Trolling? Cross-Media Amplification of Controversy at the 2012 Olympics

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    Article published with author as Billur Aslan not Billur Ozgul

    Normal operating range of the microbial community under potato

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    Om de invloed van verschillende aardappelrassen op de bodem te meten bepaalde promovenda Özgül Inceoglu de variatie in parameters van de totale microbiële populatie in de bodem onder invloed van een aantal verschillende aardappelrassen. Ook woog zij de mogelijke effecten af van het zogeheten GM-ras tegen die van geselecteerde rassen. Populaties van micro-organismen worden onder andere beïnvloed door plantenwortels. Dit gebeurt via de organische stoffen die in het door de wortels uitgescheiden exudaat zitten. Op deze manier trekken planten micro-organismen aan, die de door de plant uitgescheiden stoffen consumeren. Omdat verschillende plantensoorten en verschillende groeistadia van de plant leiden tot andere samenstellingen van wortelexudaten, is het logisch dat dit verschil ook tot uiting komt in de samenstelling van de microbiële populaties. Het is echter nog niet bekend in hoeverre zulke veranderingen voorkomen, hoe de dynamiek hiervan is en hoe deze veranderingen het functioneren van het systeem beïnvloeden. Als de invloed op de bodem van genetisch gemodificeerde planten bepaald moet worden, is het dus belangrijk om eerst de samenstelling van de microbiële populatie te bepalen, zowel als de individuele microbiële populaties en fluctuaties hierin. Hiervoor is het belangrijk, dat er eenvoudige methoden voorhanden zijn voor het monitoren van microbiële populaties en veranderingen in de tijd. Deze data vormen dan de basis voor de populatie onder landbouwgewassen in een bepaalde bodem, waaraan vervolgens de data van genetisch gemodificeerde planten gerelateerd kunnen worden, in dit geval voor de aardappel. Soil-borne microbial communities are influenced by plant roots due to, among other factors, the excretion of organic compounds in the exudates. Plants thus selectively attract microorganisms in their rhizospheres which then consume particular excreted compounds. It clearly follows that microbial communities in the rhizosphere may be differentially influenced by plant genotype as well as developmental stage if these incur different patterns of root exudation. However, there is a paucity of knowledge on the extent to which such community shifts may occur, on the dynamics of the changes and on the putative effects regarding the functioning of the system. Hence, in order to weigh effects of transgenic plants, it is important to first assess the overall community composition as well as individual microbial populations as these are affected by plants, and study fluctuations therein. Moreover, easy monitoring methods are needed to track the community and diversity changes over time. Such assessments will define the baseline of cropping in a particular soil, against which the effect of genetically modified cultivars can be weighed, in this study tuned to potato as a crop. So far, such a baseline of soil functioning has not been well-defined, and this is mainly due to the fact that soil ecosystems are dynamic and can be instantly changed due to seasonal influences as well as to different agricultural management practices. Thus, as this represents the normal operating conditions of the system, the seasonal variability of the soil communities under cropping regimes should be used to establish a baseline. The borders of these microbial aspects will be provided by the extremes of fluctuations and thus determine the normal operating conditions. This will culminate in the provision of a technically-based protocol for risk assessment of GM crops in terms of effects on soil. Since less than 1% of soil microorganisms can be cultivated from their natural environments, new and more sensitive approaches are required that provide better measures of community structure and function. However, biases of other nature are possibly introduced right at the forefront of most of our current soil DNA-based molecular analyses, that is, at the soil DNA extraction stage. Therefore in Chapter 2 of this thesis, four different DNA extraction methods, including two non-commercial ones, the Smalla et al and CIAT methods, and two commercial ones (MoBio Ultraclean and Powersoil), were compared for their efficiencies in producing DNA from three agricultural soils of contrasting texture and organic matter content. The analyses performed included assessments of DNA quality and quantity as well as PCRDGGE based analyses of bacterial, archaeal, ß-proteobacterial, ß-proteobacterial ammonium oxidizer, and pseudomonad and actinobacterial communities. Across the target bacterial groups, clear effects of DNA extraction method on the apparent richness and community structures based on DGGE patterns were discernible. Thus, different DNA extraction methods applied to the same soil sample yield different views of the communities present in that sample. Powersoil was used for the rest of project, because of its efficiency, yield and purity. In Chapter 3, the effects of plant cultivar, growth stage and soil type on the communities associated with the potato cultivars Aveka, Aventra, Karnico, Modena, Premiere and Désirée were assessed in two different sandy fields, containing either high or low amounts of organic compounds in the different growth stages (young plant, flowering, senescence).

    Validity and reliability of the Turkish Patient-Rated Wrist Evaluation questionnaire

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    Objective: The Patient-Rated Wrist Evaluation (PRWE) scale is a self-administered outcome questionnaire used to determine level of pain and disability in wrist problems. The scale includes pain (PRWE-P) and function (PRWE-F) subscales, the latter consisting of specific function (PRWE-SF) and usual (PRWE-UF) function. This study aimed to evaluate the validity and reliability of the Turkish version of the PRWE scale. Methods: Permission was sought and received from the original author of the PRWE for a Turkish translation for use in the study. The study included 110 patients (85 female and 25 male; mean age: 50.8 +/- 1.53 years; range: 18 to 85) with distal radius fracture, carpal tunnel syndrome, wrist ganglion cyst, De Quervain syndrome, Kienbock disease, and connective lesions affecting the wrist, all of whom completed the Turkish version of both the PRWE (PRWE-T) and the Disabilities of the Arm Shoulder and Hand scale (DASH). Reliability and validity of the PRWE-T scale were evaluated via an internal consistency analysis and a factor analysis respectively. The level of correlation between PRWE-T and DASH scores was also examined. Results: Cronbach's alpha coefficient was calculated as 0.86, 0.82 and 0.88 for PRWE-P, PRWE-F and PRWE-T respectively for the scale and all subscales. The PRWE-T scale was found to be highly reliable. A statistically significant correlation was found between PRWE-T and DASH in the criterion-related validity analysis (Spearman's rho=0.9). Conclusion: The PRWE-T was found to be valid and reliable. It is therefore suggested for use in evaluating patient-based pain and disability levels in routine clinical practice

    Student EFL Teachers⿿ Receptive Vocabulary Size

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    AbstractThis study reports the results of a study investigating the receptive vocabulary size of student EFL teachers. The study also examined the relationship between the participants⿿ academic achievement (departmental GPA) and their receptive vocabulary size and the existence of any gender-related difference. The study was carried out in English Language Teaching (ELT) Department of a major state university in Turkey. A total of 104 undergraduate students (females: 76 and males: 28) enrolled in the department voluntarily participated in the study, took the Version 2 of the Vocabulary Levels Tests (Schmitt, Schmitt & Clapham, 2001) and responded to a survey investigating their GPA scores and genders. The results revealed that student EFL teachers have a high level of total receptive vocabulary capacity size in their foreign language (M=103.82). Learners⿿ academic achievement (GPA) is treated as an indicator of their language proficiency level in many studies of English Language Teaching (ELT) field. However, the results of this study revealed that there was not any statistically significant correlation between academic achievement (GPA) and their receptive vocabulary size. Although GPA may be a sign of academic achievement (GPA) and successful studentship, it may not readily one's actual English language proficiency. The results also indicated that there was no significant difference between males and females regarding their receptive vocabulary size

    Cowpox virus infection in natural field vole Microtus agrestispopulations: significant negative impacts on survival

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    1. Cowpox virus is an endemic virus circulating in populations of wild rodents. It has been implicated as a potential cause of population cycles in field voles Microtus agrestis L., in Britain, owing to a delayed density-dependent pattern in prevalence, but its impact on field vole demographic parameters is unknown. This study tests the hypothesis that wild field voles infected with cowpox virus have a lower probability of survival than uninfected individuals. 2. The effect of cowpox virus infection on the probability of an individual surviving to the next month was investigated using longitudinal data collected over 2 years from four grassland sites in Kielder Forest, UK. This effect was also investigated at the population level, by examining whether infection prevalence explained temporal variation in survival rates, once other factors influencing survival had been controlled for. 3. Individuals with a probability of infection, P(I), of 1 at a time when base survival rate was at median levels had a 22.4% lower estimated probability of survival than uninfected individuals, whereas those with a P(I) of 0.5 had a 10.4% lower survival. 4. At the population level, survival rates also decreased with increasing cowpox prevalence, with lower survival rates in months of higher cowpox prevalence. 5. Simple matrix projection models with 28 day time steps and two stages, with 71% of voles experiencing cowpox infection in their second month of life (the average observed seroprevalence at the end of the breeding season) predict a reduction in 28-day population growth rate during the breeding season from λ = 1.62 to 1.53 for populations with no cowpox infection compared with infected populations. 6. This negative correlation between cowpox virus infection and field vole survival, with its potentially significant effect on population growth rate, is the first for an endemic pathogen in a cyclic population of wild rodents

    The Different Organizational Structures of Alternative Media: Through the Perspective of Alternative Media Journalists in Turkey and Greece

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    Copyright © 2021 The Author(s). This article analyses and compares the organizational structures of alternative media projects in Turkey and Greece; two countries that have recently witnessed networked social movements. Drawing on in-depth interviews with journalists working in six alternative media projects, we inform on the news production process, the news values and normative ideals adopted by these journalists while covering the news and explore if they make use of similar or different organizational structures. Our research invites a rethinking of alternative media to focus on their unique features and the differing experiences and values of their journalists. Our findings indicate that, alternative professional journalists’ news production routines in both countries vary based on their organization’s scale, normative ideals, the political and media contexts in which they operate. In this study, we reflect on what these different news production routines accomplish or fail to, and their broader implications for journalism
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