25 research outputs found
Author Correction: Data storage in DNA with fewer synthesis cycles using composite DNA letters
No full textThe magnetoelectric effect in LiFePO4 - revisited
No full textWe study the magnetoelectric effect in LiFePO4 using the pyrocurrent method to determine the magnetic -field-induced electric polarization as a function of temperature for various applied magnetic field strengths. We discover a diagonal magnetoelectric tensor element not previously reported. This new coupling reflects the emergence of an electric polarization along a induced by a magnetic field along the same direction and is consistent with the symmetry-imposed requirements for the small canting components of the magnetic ground state reported for this compound. Our observation is thus further evidence for a ground state which is not supported by the space group Pnma as originally assigned to LiFePO4.This article is published as Fogh, Ellen, Bastian Klemke, Alexandre Pages, Jiying Li, David Vaknin, Henrik M. Rønnow, Niels B. Christensen, and Rasmus Toft-Petersen. "The magnetoelectric effect in LiFePO4–revisited." Physica B: Condensed Matter 648 (2023): 414380.
DOI: 10.1016/j.physb.2022.414380.
Copyright 2022 The Author(s).
Attribution 4.0 International (CC BY 4.0).
Posted with permission.
DOE Contract Number(s): AC02-07CH11358; 81045
La vision et le leadership du maire dans le développement urbain économique durable
No full textLa recherche est centrée sur une étude de cas du développement historique de la ville d’Ashkelon pendant le mandat de l’auteur en tant que maire suppléant et maire de la ville. Axée sur l'analyse du développement durable, elle montre les effets du développement d'une vision stratégique du maire sur tous les domaines d'activité de la ville. Cette analyse montre clairement que le leadership du maire, accompagné de l’autodétermination et de la persévérance, est essentiel pour atteindre les objectifs de sa vision. L'analyse des résultats obtenus montre le succès des strategies mises en oeuvre pour soutenir la croissance urbaine, de 63 000 habitants en 1991 à 138 000 en 2017 et promouvoir son développement économique. En particulier la vision du développement économique urbain en tant que partie intégrante des fonctions de la municipalité, était essentielle pour son développement durable, ainsi que les strategies d'excellence et les normes élevées dans les domaines de l'éducation, de la culture, de la protection de l'environnement, de l’intégration sociale, le développement économique et les services municipaux. Cette recherche propose un modèle de développement durable pour des villes à forte croissance dans des conditions difficiles face aux défis du changement climatique et aux enjeux géo-politiques.This research is focused on the case study of the historical development of the City of Ashkelonduring the tenure of the author as its Deputy Mayor and subsequently as the Mayor of the City.This case study illustrates the impact of the author’s mayoral vision, as well as his leadership,self-determination and perseverance on the local governance capacity to accomplish the mayoralstrategic sustainable urban development goals, including: upgrading the performance of the widediversity of the urban service sectors, stimulating environmentally sustainable economicdevelopment, alleviating unemployment, improving the educational systems at all levels,ensuring integrated urbanization that effectively meets the societal needs of fast expandingmulti-cultural population, and promoting quality of life. Performance measures and national andlocal data statistics illustrate the significant societal, economic and environmental impacts of theprograms implemented to support the population expansion from 63,000 residents in 1991 to138,000 in 2017 and promote economic growth as a core target goal of local governance forsustainable urban development. This case study demonstrates that the mayoral leadership is amain factor in building local governance commitment to achieving excellence and promoting highperformance urbanization standards of municipal services, educational systems, socio-culturalintegration, environmental quality preservation, and other critical aspects of sustainable urbandevelopment. It proposes and illustrates the implementation of a sustainable development modelfor cities, which experience a fast population growth under extreme environmental conditionsfacing climate change impacts and geo-political stress
Author response: Multiple sources of slow activity fluctuations in a bacterial chemosensory network
No full textA universal system for boosting gene expression in eukaryotic cell-lines
No full textAbstract We demonstrate a transcriptional regulatory design algorithm that can boost expression in yeast and mammalian cell lines. The system consists of a simplified transcriptional architecture composed of a minimal core promoter and a synthetic upstream regulatory region (sURS) composed of up to three motifs selected from a list of 41 motifs conserved in the eukaryotic lineage. The sURS system was first characterized using an oligo-library containing 189,990 variants. We validate the resultant expression model using a set of 43 unseen sURS designs. The validation sURS experiments indicate that a generic set of grammar rules for boosting and attenuation may exist in yeast cells. Finally, we demonstrate that this generic set of grammar rules functions similarly in mammalian CHO-K1 and HeLa cells. Consequently, our work provides a design algorithm for boosting the expression of promoters used for expressing industrially relevant proteins in yeast and mammalian cell lines
Abstract 5027: Identification of novel immune checkpoints as potential targets for cancer immunotherapy
No full textILDR2-Fc Is a Novel Regulator of Immune Homeostasis and Inducer of Antigen-Specific Immune Tolerance
No full textAbstract
ILDR2 is a member of the Ig superfamily, which is implicated in tricellular tight junctions, and has a putative role in pancreatic islet health and survival. We recently found a novel role for ILDR2 in delivering inhibitory signals to T cells. In this article, we show that short-term treatment with ILDR2-Fc results in long-term durable beneficial effects in the relapsing-remitting experimental autoimmune encephalomyelitis and NOD type 1 diabetes models. ILDR2-Fc also promotes transplant engraftment in a minor mismatch bone marrow transplantation model. ILDR2-Fc displays a unique mode of action, combining immunomodulation, regulation of immune homeostasis, and re-establishment of Ag-specific immune tolerance via regulatory T cell induction. These findings support the potential of ILDR-Fc to provide a promising therapeutic approach for the treatment of autoimmune diseases.</jats:p
The Role of CCL24 in Primary Sclerosing Cholangitis: Bridging Patient Serum Proteomics to Preclinical Data
No full textPrimary sclerosing cholangitis (PSC) is an inflammatory and fibrotic biliary disease lacking approved treatment. We studied CCL24, a chemokine shown to be overexpressed in damaged bile ducts, and its involvement in key disease-related mechanisms. Serum proteomics of PSC patients and healthy controls (HC) were analyzed using the Olink® proximity extension assay and compared based on disease presence, fibrosis severity, and CCL24 levels. Disease-related canonical pathways, upstream regulators, and toxicity functions were elevated in PSC patients compared to HC and further elevated in patients with high CCL24 levels. In vitro, a protein signature in CCL24-treated hepatic stellate cells (HSCs) differentiated patients by disease severity. In mice, CCL24 intraperitoneal injection selectively recruited neutrophils and monocytes. Treatment with CM-101, a CCL24-neutralizing antibody, in an α-naphthylisothiocyanate (ANIT)-induced cholestasis mouse model effectively inhibited accumulation of peribiliary neutrophils and macrophages while reducing biliary hyperplasia and fibrosis. Furthermore, in PSC patients, CCL24 levels were correlated with upregulation of monocyte and neutrophil chemotaxis pathways. Collectively, these findings highlight the distinct role of CCL24 in PSC, influencing disease-related mechanisms, affecting immune cells trafficking and HSC activation. Its blockade with CM-101 reduces inflammation and fibrosis and positions CCL24 as a promising therapeutic target in PSC
Ebola Virus Glycoprotein Strongly Binds to Membranes in the Absence of Receptor Engagement
No full textEbola virus (EBOV)
is an enveloped virus that must fuse with the
host cell membrane in order to release its genome and initiate infection.
This process requires the action of the EBOV envelope glycoprotein
(GP), encoded by the virus, which resides in the viral envelope and
consists of a receptor binding subunit, GP1, and a membrane fusion
subunit, GP2. Despite extensive research, a mechanistic understanding
of the viral fusion process is incomplete. To investigate GP-membrane
association, a key step in the fusion process, we used two approaches:
high-throughput measurements of single-particle diffusion and single-molecule
measurements with optical tweezers. Using these methods, we show that
the presence of the endosomal Niemann-Pick C1 (NPC1) receptor is not
required for primed GP-membrane binding. In addition, we demonstrate
this binding is very strong, likely attributed to the interaction
between the GP fusion loop and the membrane’s hydrophobic core.
Our results also align with previously reported findings, emphasizing
the significance of acidic pH in the protein–membrane interaction.
Beyond Ebola virus research, our approach provides a powerful toolkit
for studying other protein–membrane interactions, opening new
avenues for a better understanding of protein-mediated membrane fusion
events
