1,721,044 research outputs found
Rhodanobacter fulvus sp nov., a beta-galactosidase-producing gammaproteobacterium
No full textA taxonomic study was carried out on a bacterial strain designated as Jip2(T) isolated from a soil sample mixed with rotten rice straw. It was a Gram-negative, aerobic, motile, and rod-shaped bacterium. It grew well on nutrient agar medium and utilized a fairly narrow spectrum of carbon source. The G+C content of the genomic DNA was 65.3 mol%. The major ubiquinone was Q-8. The major fatty acids were branched fatty acids, especially large amounts of iso C-15:0 and iso C-17:1 omega9c were detected in the cells grown on TSA agar for 24 h. Comparative 16S rDNA study showed a clear affiliation of this bacterium to the genus Rhodanobacter. The 16S rDNA sequence of strain Jip2(T) showed 96.4% sequence similarity to that of Rhodanobacter lindaniclasticus RP5575(T). On the basis of phenotypic characteristics and 16S rDNA sequence analysis, strain Jip2(T) is clearly distinct from Rhodanobacter lindaniclasticus. We propose the name Rhodanobacter fulvus sp. nov. for strain Jip2(T) (=IAM 15025(T) = KCTC 12098(T))
Azonexus caeni sp nov., a denitrifying bacterium isolated from sludge of a wastewater treatment plant
No full textA polyphasic taxonomic study was carried out to determine the taxonomic position of a newly isolated denitrifying bacterium, designated Slu-05(T), which had been isolated from sludge from the main aerobic treatment tanks of a municipal sewage treatment plant. Phylogenetic analysis based on comparative 16S rRNA gene sequencing indicated that strain Slu-05(T) was closely related to Azonexus fungiphilus LMG 19178(T) (96(.)4% sequence similarity), the sole species in the genus Azonexus. Strain Slu-05(T) comprised Gram-negative, motile, non-spore-forming and slightly curved rods. The predominant respiratory lipoquinone was Q-8. The major fatty acids were C-16:1 omega 7c, C-16:0, C-18:1 isomers and C-10:0 3-OH. The G+C content of the genomic DNA was 65.6 mol%. The results of DNA-DNA hybridization (15.6 %) together with phenotypic determination showed that strain Slu-5(T) could be distinguished from A. fungiphilus. Moreover, some phenotypic properties concerning enzyme activity, the substrates utilized as carbon sources and growth conditions distinguish strain Slu-5(T) from A. fungiphilus. On the basis of the results obtained in this study, Slu-05(T) (=DSM 17719(T) =KCTC 12530(T) = CCBAU 10199(T)) is the type strain of a novel species of Azonexus, for which the name Azonexus caeni sp. nov. is proposed
Pedobacter panaciterrae sp nov., isolated from soil in South Korea
No full textA Gram-negative, facultatively anaerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 042(T), was isolated from a soil sample of a ginseng field in Pocheon province (South Korea) and was characterized taxonomically by using a polyphasic approach. The isolate was positive for catalase and oxidase, but negative for gelatinase and production of indole and H2S. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acids were summed feature 4 (C-16:1 omega 7c and/or iso-C-15:0 2-OH; 35.7%), iso-C-15:0 (24.3%) and iso-C-17:0 3-OH (11.6%). Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 042(T) fell within the radiation of the cluster comprising Pedobacter species. On the basis of 16S rRNA gene sequence similarities, the phylogenetically closest recognized species of the genus Pedobacter to strain Gsoil 042(T) included Pedobacter heparinus DSM 2366(T) (98.1%) Pedobacter africanus DSM 12126(T) (97.8%)and Pedobacter caeni LMG 22862(T) (97.2%) The 16S rRNA gene sequence similarity with other recognized species of the genus Pedobacter was less than 96.1%. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness between strain Gsoil 042(T) and its phylogenetically closest neighbours was less than 43%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 042(T) should be classified as representing a novel species in the genus Pedobacter, for which the name Pedobacter panaciterrae sp. nov. is proposed. The type strain is Gsoil 042(T) (= KCTC 12594(T) = LMG 23400(T))
Pseudoxanthomonas koreensis sp nov and Pseudoxanthomonas daejeonensis sp nov.
No full textGram-negative, non-spore-forming, rod-shaped bacteria, T7-09(T) and TR6-08(T), were isolated from soil from a ginseng field in South Korea and characterized to determine their taxonomic position. 16S rRNA gene sequence analysis showed that the two isolates shared 99-5% sequence similarity. Strains T7-09(T) and TR6-08(T) were shown to belong to the Proteobacteria and showed the highest levels of sequence similarity to Pseudoxanthomonas broegbernensis DSM 12573(T) (98-1%), Pseudoxanthomonas mexicana AMX 26B(T) (97-4-97.5%), Pseudoxanthomonas japonensis 12-3(T) (96-5-96-6%), Pseudoxanthomonas taiwanensis ATCC BAA-404(T) (95.7 %) and Xanthomonas campestris ATCC 33913(T) (96.3-96.5%). The sequence similarity values with respect to any species with validly published names in related genera were less than 96.5 %. The detection of a quinone system with Q-8 as the predominant compound and a fatty acid profile with C15:0 iso as the predominant acid supported the assignment of the novel isolates to the order ' Xanthomonadales '. The two isolates could be distinguished from the established species of the genus Pseudoxanthomonas by the presence of quantitative unsaturated fatty acid C-17:1 iso omega 9c and by their unique biochemical profiles. The results of DNA-DNA hybridization clearly demonstrated that T7-09(T) and TIR6-08(T) represent separate species. On the basis of these data, it is proposed that T7-09(T) (= KCTC 12208(T) = lAM 15116(T)) and TR6-08(T) (=KCTC 12207(T) = IAM 15115(T)) be classified as the type strains of two novel Pseudoxanthomonas species, for which the names Pseudoxanthomonas koreensis sp. nov. and Pseudoxanthomonas daejeonensis sp. nov., respectively, are proposed
Herbaspirillum chlorophenolicum sp nov., a 4-chlorophenol-degrading bacterium
No full textA 4-chlorophenol-degrading bacterial strain, formerly designated as a strain of Comamonas testosteroni, was reclassified as a member of the genus Herbaspirillum based on its phenotypic and chemotaxonomic characteristics, as well as phylogenetic analysis using 16S rDNA sequences. Phylogenetic inference based on 16S rDNA sequences showed that strain CPW301(T) clusters in a phylogenetic branch that contains Herbaspirillum species. 16S rDNA sequence similarity of strain CPW301(T) to species of the genus Herbaspirillum with validly published names is in the range 98.7-98.9%. Despite the considerably high 16S rDNA sequence similarity, strain CPW301(T) could be distinguished clearly from type strains of Herbaspirillum species with validly published names by DNA-DNA relatedness values, which were < 15.7%. The genomic DNA G + C content of strain CPW301(T) is 61.3 mol%. The predominant ubiquinone is Q-8 and the major cellular fatty acids are C-16:0 and Cyclo-C-17:0. The strain does not fix nitrogen and is not plant-associated. It is an aerobic rod with one unipolar flagellum. On the basis of these characteristics, a novel Herbaspirillum species, Herbaspirillum chlorophenolicum sp. nov., is proposed. The type strain of the novel species is strain CPW301(T) (= KCTC 12096(T) = IAM 15024(T))
Phenylobacterium koreense sp nov., isolated from South Korea
No full textT A Gram-negative, aerobic, rod-shaped, non-spore-forming bacterium, Slu-01(T) , was isolated from activated sludge from a wastewater treatment plant in Daejeon, South Korea. After 3 days on R2A medium, strain Slu-01(T) developed colourless colonies (0(.)-7-1(.)2 mm). The non-motile rods (0(.)7-1(.)0 x 1(.)0-2(.)0 mu m) were slightly curved and occurred singly and in pairs. No filamentous cells were found. On the basis of 16S rRNA gene sequence similarity, strain SlU-01(T) was shown to belong to the family Caulobacteraceae and was most closely related to Phenylobacterium immobile (96(.)6 %) and Phenylobacterium lituiforme (96(.)5 %). The genomic DNA G + C content of strain SlU-01(T) was 68(.)1 mol%, within the range of 67(.)3-68(.)4 mol% for the genus Phenylobacterium. Chemotaxonomic data (major ubiquinone, Q-10; major fatty acids C(18:1)omega 7c, C-16:0, C-15:0 and C-17:0) and 16S rRNA gene sequence analysis supported the affiliation of strain SlU-01(T) to the genus Phenylobacterium. However, DNA-DNA hybridization data and phenotypic properties showed that strain Slu-01(T) could be distinguished from the two other Phenylobacterium species with validly published names. Thus, it is suggested that strain Slu-01(T) represents a novel species of the genus Phenylobacterium, for which the name Phenylobacterium koreense is proposed. The type strain is SlU-01(T) (=KCTC 12206(T) = IAM 15119(T))
Flavobacterium granuli sp nov., isolated from granules used in a wastewater treatment plant
No full textA Gram-negative, rod-shaped, non-spore-forming bacterium (designated strain Kw05(T)) was isolated from granules used in the wastewater treatment plant of a beer-brewing factory in Kwang-Ju, Republic of Korea. On the basis of 16S rRNA gene sequence similarity, strain Kw05(T) was shown to belong to the family Flavobacteriaceae, and was most closely related to Flavobacterium limicola (96-6%), Flavobacterium hibernum (96-3%), Flavobacterium hydatis (96-1%) and Flavobacterium xinjiangense (96-1%). The G+C content of the genomic DNA of strain Kw05(T) was 36-2 mol%, within the range of 32-37 mol% for the genus Flavobacterium. Chemotaxonomic data (major menaquinone MK-6; major fatty acids iso-C-15: 0, isoC(17: 0) 3-OH, iso-C-15:0 3-OH and iso-C-17:1 omega 9C) supported the classification of strain Kw05(T) within the genus Flavobacterium. Kw05(T) therefore represents a novel species, for which the name Flavobacterium granuli sp. nov. is proposed. The type strain is Kw05(T) ( = KCTC 12201(T)= IAM 15099(T))
Methylibium fulvum sp nov., a member of the Betaproteobacteria isolated from ginseng field soil, and emended description of the genus Methylibium
No full textTwo bacterial strains, designated Gsoil 322 T and Gsoil 328, were isolated from soil of a ginseng field in Pocheon province (Republic of Korea). The strains were Gram-negative, motile, aerobic rods that showed nearly identical physiological profiles and similar chemotaxonomic profiles. The two strains were oxidase-positive but catalase-negative, reduced nitrate to nitrite and had fatty acid profiles in which C-16:0, C-17:0 cyclo and C-16:10 omega 7c/iso-C-15:0 2-OH were predominant. The DNA G + C contents of Gsoil 322(T) and Gsoil 328 were 66.6 and 66.7 mol%, respectively. Q-8 was observed as the major quinone. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 322 T belongs to the class Betaproteobacteria and was most closely related to Methylibium petroleiphilum ATCC BAA-1232(T) (97.5% sequence similarity). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 322(T) (=KCTC 12591(T) =LMG 23394(T)) was classified in the genus Methylibium as the type strain of a novel species, for which the name Methylibium fulvum sp. nov. is proposed
Kaistia adipata gen. nov., sp nov., a novel alpha-proteobacterium
No full textA taxonomic study was carried out on Chj404(T) (dagger), a bacterial strain isolated from a soil sample collected in an industrial stream near the Chung-Ju industrial complex in Korea. The strain was a gram-negative, aerobic, short rod to coccus-shaped bacterium. It grew well on nutrient agar medium and utilized a broad spectrum of carbon sources. The G+C content of the DNA was 67.4 mol% and the major composition of ubiquinone was Q-10. The major fatty acid was C-18:1. Comparative 16S rDNA studies showed a clear affiliation of this bacterium to alpha-Proteobacteria. Comparison of phylogenetic data indicated that it was most closely related to Prosthecomicrobium pneumaticum (92.7% similarity in 16S rDNA sequence). Since strain Chj404 is clearly distinct from closely related species, we propose the name Kaistia adipata gen. nov., sp. nov. for this strain Chj404(T) (=IAM 15023(T) =KCTC 12095(T))
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