1,721,077 research outputs found
Editorial Comment on Anatomical outcomes and complications of sacrocolpopexy using surelift uplift mesh: A multicentric observational study
No full textRe: Long-term Results of Burch and Autologous Sling Procedures for Stress Urinary Incontinence in E-SISTEr Participants at 1 Site
No full textAUTHOR REPLY
No full textAs you have pointed out the main conclusion drawn from our
data is that, for the time being, the choice between laparoscopic
and robot-assisted surgery can only be based on cost, time, and
training requirements, since there is consistently no difference in
efficacy and safety in available trials. When longer follow-up
becomes available, durability of results could be compared and
further guide our choice
Are there the differences in the anatomical and functional outcomes between hysterectomy and uterus preservation during sacrocolpopexy?
No full textComparison between laparoscopic sacrocolpopexy with hysterectomy and hysteropexy in advanced urogenital prolapse
No full textIntroduction and hypothesis: The aim of this study was to compare the outcomes in women who underwent laparoscopic sacrocolpopexy (LSC) with or without hysterectomy for pelvic organ prolapse (POP). Methods: This was a single-centre prospective study. We included women with symptomatic POP (III–IV stage) who underwent LSC with or without hysterectomy. The preoperative evaluation included a history, clinical examination and urodynamic test; all patients completed FSFI, UDI-6 and IIQ-7 questionnaires. They were followed up at 1, 3, 6 and 12 months after surgery and then annually thereafter with the same preoperative flow chart. At the last visit, they also completed the PGI-I questionnaire. Results: Between 2012 and 2016, a total of 136 patients with POP were included (82 in the LSC with hysterectomy group and 54 in the hysteropexy group). At a median follow-up of 65.3 months (36–84 months), there were improvements in the anatomical and functional outcomes of both groups without differences between the two approaches. The apical success rate was 100% in all women, without recurrence in either group; the anterior and posterior success rates of hysterectomy were higher than those of uterine preservation. Conclusion: This study showed that there were no differences in the anatomical and functional outcomes between LSC with or without hysterectomy for POP
Production of functional, stable, unmutated recombinant human papillomavirus E6 oncoprotein: Implications for HPV-tumor diagnosis and therapy
No full textBackground: High-risk human papillomaviruses (HR-HPVs) types 16 and 18 are the main etiological agents of cervical cancer, with more than 550,000 new cases each year worldwide. HPVs are also associated with other ano-genital and head-and-neck tumors. The HR-HPV E6 and E7 oncoproteins are responsible for onset and maintenance of the cell transformation state, and they represent appropriate targets for development of diagnostic and therapeutic tools. Methods: The unmutated E6 gene from HPV16 and HPV18 and from low-risk HPV11 was cloned in a prokaryotic expression vector for expression of the Histidine-tagged E6 protein (His6-E6), according to a novel procedure. The structural properties were determined using circular dichroism and fluorescence spectroscopy. His6-E6 oncoprotein immunogenicity was assessed in a mouse model, and its functionality was determined using in vitro GST pull-down and protein degradation assays. Results: The His6-tagged E6 proteins from HPV16, HPV18, and HPV11 E6 genes, without any further modification in the amino-acid sequence, were produced in bacteria as soluble and stable molecules. Structural analyses of HPV16 His6-E6 suggests that it maintains correct folding and conformational properties. C57BL/6 mice immunized with HPV16 His6-E6 developed significant humoral immune responses. The E6 proteins from HPV16, HPV18, and HPV11 were purified according to a new procedure, and investigated for protein-protein interactions. HR-HPV His6-E6 bound p53, the PDZ1 motif from MAGI-1 proteins, the human discs large tumor suppressor, and the human ubiquitin ligase E6-associated protein, thus suggesting that it is biologically active. The purified HR-HPV E6 proteins also targeted the MAGI-3 and p53 proteins for degradation. Conclusions: This new procedure generates a stable, unmutated HPV16 E6 protein, which maintains the E6 properties in in vitro binding assays. This will be useful for basic studies, and for development of diagnostic kits and immunotherapies in preclinical mouse models of HPV-related tumorigenesis. © 2016 The Author(s)
Antibiotic prophylaxis or antibiotic treatment in prolapse surgery? A dilemma in the age of antibiotic resistance
No full textTHE PREOPERATIVE URINARY DYSFUNCTIONS:CAN THEY IMPROVE AFTER LAPAROSCOPIC SACROCOLPOPEXY?
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PROSPECTIVE RANDOMIZED CONTROLLED TRIAL COMPARING THE EFFECT OF TOTAL VS SUBTOTAL HYSTERECTOMY ASSOCIATED WITH LAPAROSCOPIC COLPOSACROPEXY
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