1,721,053 research outputs found
Human CD4 internal antigen anti-idiotypic monoclonal antibody. Immunochemical and sequence analysis
No full textThe mouse mAb2 16D7 recognizes the paratope of the syngeneic anti-human CD4 mAb HP2/6 (mAb1 of our idiotypic cascade) and mimics CD4 in xenogeneic settings in humans. Immunochemical and sequence analyses were performed to define the minimum structural requirement for this mimicry. Binding assay of mAb1 with isolated naive 16D7 H and L chains showed that only the second reacted with mAb1. Specificity was indicated by the lack of reactivity of mAb1 with the L chain of mAb2 14D6, which also recognizes mAb1-paratope. It is likely that the 16D7-L mAb1-specific epitope is "sequence-dependent", since fully denatured 16D7-L still reacted with mAb1. Sequence analysis of 16D7 and mAb1 showed a high degree of homology of their VH, as both were coded by the same gene family (V/II), whereas CDR3 showed the greatest diversity. Alignment of 16D7-H CDR3 with CD4, however, produced no similarity. In contrast, analyses of the 16D7 VL sequence (XX/V) defined a CDR3 6-mer peptide with a 50% identity (83% of similarity) to the CD4 stretch 218-223. This peptide seems a suitable replacement for 16D7 in active immunotherapy as it did not match any protein fragment retrieved from the n-r database (NCBI) and both the peptide and the corresponding CD4 amino acid stretch are surface accessible. Based on their immunochemical profiles and similarity to CD4, four additional 16D7-derived peptides were designed for synthesis. The data indicate that CD4 mimicry by mAb2 can be obtained at the level of primary structure and provide useful information for the synthesis of peptide(s) with bioactive potential
CD4 mimicry by anti-idiotypic mAb (mAb2) F16-16D7 (16D7). Immunochemical and sequence analysis
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Subclass and clonotypic profiles of serum AB elicited with the "human CD4 internal AG" mab F16-16D7 (16D7) in 4 patients with systemic lupus erythematosus
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In vitro enhancement of the proliferative response of human T cells to autologous non-T cells by hydralazine.
No full textAnti-idiotypic monoclonal antibody (mAb) to anti-CD4 mAb. Potential treatment of autoimmune diseases
No full textT cells from patients with chronic liver diseases: abnormalities in PHA-induced expression of HLA class II antigens and in autologous mixed-lymphocyte reactions.
No full textMurine anti-idiotypic monoclonal antibodies (mAb2) to an anti-CD4 monoclonal antibody induces anti-CD4 antibodies in humans
No full textComplete remission of AIDS/KS after treatment with a combination of two NRTIs and one NNRTI
No full textAnalisi funzionale e fenotipica dei T linfociti isolati dal sangue periferico di pazienti affetti da AIDS
No full textThe diagnosis of polymyalgia rheumatica in patients with a low erythrocyte sedimentation rate: comment on the article by Helfgott and Kieval.
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