115 research outputs found
Gyromastix Doweld, 2021, nom. nov.
No full textGenus Gyromastix nom. nov. pro Peregrinia Cavalier-Smith 2011. Diagnosis: see Howe et al. (2011: p. 349). Etymology: From Greek γύρος (gýros), from the turning of the meat on a spit, and Greek μάστιξ (mastix), for the whip form (scourge). Type species: Gyromastix clavideferens (Howe, Arndt & Cavalier-Smith) Doweld, comb. nov. ≡ Peregrinia clavideferens Howe, Arndt & Cavalier-Smith, 2011.Published as part of Doweld, Alexander B., 2021, Gyromastix, a new name for Peregrinia Cavalier-Smith, 2011 (Protista: Thaumatomonada) non Peregrinia E. I. Vorobyeva & O. A. Lebedev, 1986 (Pisces Osteolepididae), pp. 399-400 in Zootaxa 5072 (4) on page 399, DOI: 10.11646/zootaxa.5072.4.8, http://zenodo.org/record/574899
Clinical experience with recombinant prourokinase for complex retinal diseases in type 2 diabetes
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Background: medical treatment of vitreous and retinal hemorrhages is a relevant issue. Considering this, the authors review published data of different years. Fibrinolytic enzymes are reasonable for vitreous and retina hemorrhages treatment from the viewpoint of pathogenesis.
Aim: to improve treatment strategies for complex retinal diseases (i.e., diabetic retinopathy, hypertensive retinopathy, “wet” age-related macular degeneration (AMD) with hemorrhages in type 2 diabetes) using recombinant prourokinase by dynamic retinal changes and tear levels of vascular endothelial growth factor (VEGF-A).
Patients and Methods: 30 patients (30 eyes) with diabetic retinopathy, hypertensive retinopathy, and AMD were examined. All patients were subdivided into 4 groups. Best-corrected visual acuity (BCVA), retinal light sensitivity (using MAIA microperimetry), vitreous hemorrhage severity (using vitreous hemorrhage density grading scale), intraocular pressure, and tear levels of VEGF-A (by ELISA) were measured.
Results: after treatment with recombinant prourokinase, VEGF-A level was 1520–1640 pg/mL. Ocular parameters (i.e., BCVA, retinal light sensitivity, and vitreous hemorrhage severity) and eye fundus manifestations are likely to benefit from recombinant prourokinase. It was demonstrated that recombinant prourokinase is highly effective for vitreous and retinal hemorrhages in patients with complex retinal diseases.
Conclusion: fibrinolytic treatment provided stabilization of pathological process in patients with complex retinal diseases since VEGF-A tear levels did not change significantly. The result of standard treatment was significant increase in VEGF-A levels thus demonstrating the activity of pathological process despite the treatment. Single intravitreal injection of recombinant prourokinase (0.2 mL, 500 IU) on day 3 after the disease begins and further daily parabulbar injections of recombinant prourokinase (5000 IU/0.5 mL of sodium chloride 0.9%) of 10 injections are recommended for complex retinal diseases (i.e., vitreous, retinal hemorrhages) in type 2 diabetes.
Keywords: diabetic retinopathy, hypertensive retinopathy, age-related macular degeneration, vascular endothelial growth factor, fibrinolytic, fibrinolytic treatment, recombinant prourokinase, Gemase.
For citation: Moshetova L.K., Slonimsky Yu.B., Vorobyeva I.V. et al. Clinical experience with recombinant prourokinase for complex retinal diseases in type 2 diabetes. Russian Journal of Clinical Ophthalmology. 2019;19(2):73–79.
About the authors:
1Larisa K. Moshetova — MD, PhD, Professor, Academician of the Russian Academy of Sciences, Head of Ophthalmology Department, ORCID iD 0000-0002-5899-2714;
1Yury B. Slonimsky — MD, PhD, Professor of the Department of Ophthalmology, ORCID iD 0000-0002-5656-4919;
1Irina V. Vorobyeva — MD, PhD, Associate Professor of the Department of Ophthalmology, ORCID iD 0000-0003-2707-8417;
1Ana Dgebuadze — postgraduate student of the Department
of Ophthalmology, ORCID iD 0000-0002-9518-0459;
2Olga V. Agafonova — PhD in Biology, Research Scientist, ORCID iD 0000-0002-7483-7167;
2Evgeny P. Delver — PhD in Biology, Senior Research Scientist, ORCID iD 0000-0003-2319-7937;
2Anatoly A. Belogurov — PhD in Biology, Leading Research Scientist, ORCID iD 0000-0002-7170-9530.
1Russian Medical Academy of Continuous Professional Education. 2/1, Barrikadnaya str., Moscow, 125993, Russian
Federation.
2Research Institute of experimental cardiology of the National Medical Research Center of Cardiology. 15а, 3rd Cherepkovskaya str., Moscow, 121552, Russian Federation.
Contact information: Irina V. Vorobyeva, e-mail: [email protected]. Financial Disclosure: no author has a financial or property interest in any material or method mentioned. There is no conflict of interests. Received 15.04.2019.
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Gyromastigidae Doweld 2021, fam. nov.
No full textFamily GyRomastiGidae Doweld fam. nov. Diagnosis: Cells with oval two-tiered body scales, lacking flagellar scales; scales either symmetric ovals with heavily outturned upper and lower rims (Gyromitus) or asymmetric ovals with concave to flat lower surface and convex upper surface with rims more strongly laterally inrolled than at the ends (Gyromastix). Flagellar pit apical or not subapical and ventral, or cells so amoeboid as to lack defined shape; flagella not clearly differentiated into anterior and posterior; flagellar gliding unknown; locomotion by swimming or slow amoeboid creeping. Type genus: Gyromastix nom. nov. Included genera: Gyromastix nom. nov.; Gyromitus Skuja 1939.Published as part of Doweld, Alexander B., 2021, Gyromastix, a new name for Peregrinia Cavalier-Smith, 2011 (Protista: Thaumatomonada) non Peregrinia E. I. Vorobyeva & O. A. Lebedev, 1986 (Pisces Osteolepididae), pp. 399-400 in Zootaxa 5072 (4) on page 399, DOI: 10.11646/zootaxa.5072.4.8, http://zenodo.org/record/574899
Gyromastix limax Doweld 2021, sp. nov.
No full textGyromastix limax Doweld, sp. nov. ≡ Gyromitus limax Swale & Belcher, Arch. Protistenk. 117: 20–21. 1975, nom. inval. [ICBN] (nom. unavail.). [ICZN] ≡ Peregrinia limax (Swale & Belcher) Cavalier-Smith, 2011, comb. inval. & nom. unavail. [ICZN, ICBN] Diagnosis: see Swale & Belcher (1975: 20–21). Holotype: (iconotype) Swale & Belcher (1975: pl. 1–3). Note: Since Gyromitus limax was originally published as a botanical taxon [ICBN, Turland et al., 2018], the rules of botanical nomenclature were applied to the nomenclature of the flagellate microorganism. Authors unhappily did not designate the necessitated holotype of the species, required by the Botanical Code from 1958, and hence the species was not validly published (ICBN, now ICN, Art. 40.1). When Cavalier-Smith (2011) recombined the species in Peregrinia, the invalid basionym name was formally recombined as Peregrinia with no rectifying actions as to its invalid nomenclatural status. Herewith the species is validated to conform to the rules of both ICN and ICZN to allow its acceptance and valid/ available use as an ambiregnal microorganism.Published as part of Doweld, Alexander B., 2021, Gyromastix, a new name for Peregrinia Cavalier-Smith, 2011 (Protista: Thaumatomonada) non Peregrinia E. I. Vorobyeva & O. A. Lebedev, 1986 (Pisces Osteolepididae), pp. 399-400 in Zootaxa 5072 (4) on pages 399-400, DOI: 10.11646/zootaxa.5072.4.8, http://zenodo.org/record/574899
A case of mistaken identity: ‘<i>Latvius</i>’ <i>obrutus</i> and new dipnoans from the Frasnian of Stolbovo, Russia
No full textThe village of Stolbovo, western Russia, has previously yielded a dipnoan fauna comprising the long-snouted form 'Rhinodipterus' stolbovi, but also a three-dimensionally preserved otoccipital region of a skull previously identified as the osteolepiform ‘Latvius’ obrutus. This identification was made from the density of lateral line canal pores in the skull roof bones in addition to what Vorobyeva (1977) described as the post-parietal, tabular and extratabular bones. Reexamination of this specimen does not recognise the osteolepiform dermal elements previously identified but rather a dipnoan configuration including a characteristic B-bone, lateral dermal elements and a dipnoan parasphenoid. rhinodipterid and dipterid dipnoans. Phylogenetic analysis resolves ‘Latvius’ obrutus as the primitive sister taxon to the ‘phaneropleurid-fleurantiid’ clade – a clade comprising derived Middle–Upper Devonian lungfishes. 'Rhinodipterus' stolbovi resolves among the more primitive griphognathids, typical Gondwanan forms, and is not a rhinodipterid. Additional isolated dipnoan skeletal elements from the site include tooth plates, parasphenoids, shoulder girdle elements and dermal elements and indicate at least an additional three lungfish taxa from Stolbovo. Of particular note is a ctenodiform tooth plate more characteristic of Carboniferous lungfishes. The new material and analyses not only demonstrate increased lungfish diversity from the Frasnian of Baltica but also probable interchange between Gondwana and Baltica during this time. Most importantly, Carboniferous-like forms appear more common in the Devonian than previously realised indicating that Carboniferous lungfishes likely represent a survival assemblage in addition to a new radiation of lungfish.</jats:p
<i>In vivo</i> activation of NCL probe by luciferase and nitroredictase expressing <i>E</i>.<i>coli</i> in a mouse model of thigh infection.
No full text<p>(A). Luminescence over 4 h from <i>E</i>. <i>coli luc+</i> infected quadriceps (5 × 10<sup>4</sup>–5 × 10<sup>7</sup> bacteria) after IP injection of 0.8 mg NCL probe (200 μL of 10 mM solution in PBS). (B). Luminescence over 4 h from <i>E</i>. <i>coli luc+</i> infected quadriceps (5 × 10<sup>7</sup> bacteria) following IP injection of 0.8 mg of probe or 0.63 mg of luciferin (200 μL of 10 mM solution in PBS). (C). Luminescence imaging of mice over 24 h bearing 5 × 10<sup>7</sup> bacteria, treated with various (0.08, 0.8 and 1.6 mg) NCL probe concentrations (200 μL of 1, 10 and 20 mM solutions of NCL in PBS). As a positive control, mice were injected with equal amounts of <i>E</i>. <i>coli</i> MG1655 expressing lux luciferase that doesn't require exogenous substrate for light production [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131037#pone.0131037.ref044" target="_blank">44</a>]. The signal was collected over 24 h, n = 3 per group.</p
Chemical composition of the hydro-cryogenic system of the Lake Baikal: “snow on ice–ice–water under-ice”
No full textThe results of studies of the chemical composition and the basic regularities of migration of macro- and microelements within the hydro-cryogenic system “snow on ice–ice–water under ice” obtained in the winter of 2016/17 in the waters of Lake Baikal are presented. Such investigation over the Lake area was carried out for the first time. It has been found that due to climatic conditions, dates of freeze-up (formation of the ice on the Lake) differ by 7–10 days from North to South, and the depth of snow on ice and its density change over the Lake area by 2 times, however there are some parts without snow. It was found that the changes in the pH indexes were identical across the whole Lake area – minimum pH values are present in the snow (from 5.59 to 7.39), average values – in the ice (6.01–7.50), and maximum values are noted in the water under ice (7.42–8.50). For the most part, increased quantities of suspended matter and an increase in the pH of snow were recorded near settlements, which is obviously a result of the anthropogenic influence. It was determined that the concentration of ions in the ice in relation to their content in the initial solutions decreases within the series: NO2–> Cl – >SO42– >HCO3– . Among the cations, K+, Na+ ions are involved into the ice intensively, while the Ca2+ and Mg2+ – weakly. The ice phase is enriched with ammonium ions outside the settlements. Near settlements and in shallow water, quantity of salts in the ice may be close to or equal to their concentration in the water under ice. The coefficient of migration in the water (Kx) divide the chemical elements into two groups – the mobile ones and slow-moving elements. The first group includes Ca, Cu, Sr, Mg, Co, Zn, and Cd (Kx >1), the second one contains Ba, Mn, Si, Fe, Al, Ti, Ni, Cr, P, and K
El género Rhaponticum en el Este de Asia.
Get PDF6p.[EN] The genus Rhaponticum in East Asia has always been a taxon for discussion. Rhaponticum carthamoides from East
Siberia comprises three subspecies: carthamoides, chamarensis and orientale. Even though they differ in morphology,
they do not have isolated areas. Rhaponticum satzyperovii was recently described and its author pointed out its
affinity with Rh. uniflorum. Plant height, stem indumentum, and radical and stem leaf dissection were signaled as
the diagnostic characters. Our present study on living and herbarium specimens of Rh. satzyperovii shows that the
diagnostic characters are not consistent. The species area was also claimed to be an argument for considering Rh.
satzyperovii a distinct species. This area covers the south of the Primorye Province in the Far East of Russia with
some locations in the adjacent Jewish Autonomous Region and in China. In our study, the area of Rh. satzyperovii is
found to be within the area of Rh. uniflorum and thereafter they turned out to have no disjunction. In East Asia, Rh.
uniflorum is characterized by a wide range of morphological variability. We suggest that Rh. satzyperovii should be
included within Rh. uniflorum without any taxonomic rank.[ES] discutido. Rhaponticum carthamoides del Este de Siberia incluye tres subespecies: carthamoides, chamarensis y
orientale. Aunque difieren en su morfología, sus áreas no están aisladas. Rhaponticum satzyperovii fue descrito recientemente
y su autor señaló su afinidad con Rh. uniflorum. Los caracteres diagnósticos fueron la altura de la planta,
el indumento del tallo y las divisiones de las hojas basales y caulinares. Nuestro estudio de plantas vivas y muestras
de herbario de Rh. satzyperovii muestra que los caracteres diagnósticos no son consistentes. El área de distribución
también se argumentó para considerar Rh. satzyperovii una especie diferente. El área cubre el sur de la provincia de
Primorye en el extremo Este de Russia con algunas localidades en la adyacente región Autónoma Judía y en China.
En nuestro estudio hemos visto que el área de Rh. satzyperovii está dentro del área de Rh. uniflorum y por lo tanto
no cabe hablar de disyunción. En el Este de Asia, Rh. uniflorum se caracteriza por un amplio rango de variabilidad
morfológica. Proponemos que Rh. satzyperovii se incluya en Rh. uniflorum sin ningún rango taxonómico.This research was supported by the Advice of grants of the President
of the Russian Federation for support of young Russian
scientists and leading scientific schools (projects MK-4620.2006.4
and 8969.2006.4) and the integration grant of the Siberian department
of the Russian Academy of Sciences (06-II-SD-05-021).Peer reviewe
Superposition of the active site residues of Class I Fba.
No full textThe predicted active site topology of the E. coli enzyme (derived from I-Tasser, Model 1) is shown in dark green on both figure panels. A. Spatial alignment of E. coli FbaB and the X-ray crystal structure of FbaB from Thermoproteus tenax (PDB: 1W8R [52]). B. The alignment between E. coli FbaB relative to Oryctolagus cuniculus FbAB (PDB: 1ZAI [9]). The substrate fructose-1,6-bisphosphate (FBP), taken from the 1W8R crystal structure, is represented as a ball-and-stick in panel A. The amino acid numbers of the corresponding proteins are given in black for the X-ray crystal structures and in green for the I-Tasser E.coli model.</p
Imaging of NTR activity in cells and in <i>in vivo</i> cancer model with NCL.
No full text<p>(A) Concentration-dependent uncaging of NCL in MDA-MB-231 NTR+luc+ cancer cells in comparison with luciferin. (B) Selectivity of NTR imaging by NCL in the same cells in comparison with NTR-luc+ cells. The dashed line indicates background (cells only), *P = 0.0001. (C) <i>In vivo</i> imaging of NTR activity in subcutaneous NTR+ and NTR- xenografts (n = 5). Total luminescence over 1 h from IP injection of luciferin (1.5 mg) and NCL (1.9 mg). d) Representative images of mice 15 min post injection of luciferin or NCL.</p
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