21,244 research outputs found
The characteristics of free <i>Rg</i> PAL and MCM-41-NH-GA-<i>Rg</i> PAL.
No full text<p>(a) The optimal pH of free <i>Rg</i>PAL and MCM-41-NH-GA-<i>Rg</i>PAL. The effects of pH were determined at 50°C using a series of buffers with various pH values. (b) The pH stability of free and immobilized <i>Rg</i>PAL. The activity was assessed following the enzyme treatment in buffer at different pH values (5.0–12) for 12 h. The relative activity at pH 8.5 was defined as 100%. (c) The optimal temperature of free and MCM-41-NH-GA-<i>Rg</i>PAL. (d) The thermostability of free <i>Rg</i>PAL and MCM-41-NH-GA-<i>Rg</i>PAL. The free <i>Rg</i>PAL and MCM-41-NH-GA-<i>Rg</i>PAL were incubated at 60°C for 10–60 min. The relative activity before incubation was defined as 100%. The values presented correspond to the mean values of at least three replicates.</p
Plastid Genotyping Reveals the Uniformity of Cytoplasmic Male Sterile-T Maize Cytoplasms
No full textCytoplasmic male-sterile (CMS) lines in maize (Zea mays) have been classified by their response to specific restorer genes into three categories: cms-C, cms-S, and cms-T. A mitochondrial genome representing each of the CMS cytotypes has been sequenced, and male sterility in the cms-S and cms-T cytotypes is linked to chimeric mitochondrial genes. To identify markers for plastid genotyping, we sequenced the plastid genomes of three fertile maize lines (B37, B73, and A188) and the B37 cms-C, cms-S, and cms-T cytoplasmic substitution lines. We found that the plastid genomes of B37 and B73 lines are identical. Furthermore, the fertile and CMS plastid genomes are conserved, differing only by zero to three single-nucleotide polymorphisms (SNPs) in coding regions and by eight to 22 SNPs and 10 to 21 short insertions/deletions in noncoding regions. To gain insight into the origin and transmission of the cms-T trait, we identified three SNPs unique to the cms-T plastids and tested the three diagnostic SNPs in 27 cms-T lines, representing the HA, I, Q, RS, and T male-sterile cytoplasms. We report that each of the tested 27 cms-T group accessions have the same three diagnostic plastid SNPs, indicating a single origin and maternal cotransmission of the cms-T mitochondria and plastids to the seed progeny. Our data exclude exceptional pollen transmission of organelles or multiple horizontal gene transfer events as the source of the mitochondrial urf13-T (unidentified reading frame encoding 13-kD cms-T protein) gene in the cms-T cytoplasms. Plastid genotyping enables a reassessment of the evolutionary relationships of cytoplasms in cultivated maize.Peer reviewe
Structural properties of <i>Rx</i>PAL by homology modeling.
No full text<p>Ribbon representation of the homotetrameric model of <i>Rx</i>PAL (Panel A) and backbone line representation model with the MIO (in pink) surrounded by three pairs of cysteines (in yellow) which may form two intra-subunit and one inter-subunit disulfide bridges (Panel B). The electrostatic surface potential representation of <i>Rx</i>PAL (Panel C) compared to that of less alkalophilic <i>Av</i>PAL (Panel D, PDB code 3CZO <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085943#pone.0085943-Wang1" target="_blank">[35]</a>) indicated highly negatively charged <i>Rx</i>PAL surface.</p
MALDI-TOF mass spectra from <i>Pseudoalteromonas (PAL)</i> isolates.
No full text<p>The mass spectrum of <i>P. tetraodonis</i> was added to the database after identification of an isolate by 16S rRNA gene sequencing. Isolate S49, with some similarities to the mass spectral pattern of <i>PAL</i> is also included in the comparisons. Panel A shows spectra of all <i>PAL</i> isolates for the range of <i>m/z</i> 3000–10000. Panels B to E show similarities and differences between the <i>PAL</i> isolates at different <i>m/z</i>. The order of isolates in all panels is as labelled in panel A.</p
Expression and purification of <i>Rx</i>PAL.
No full text<p>Bacterial cells transformed with the pBAD-HisB plasmid containing the gene for <i>Rx</i>PAL were extracted and the cell extract was run on 12% SDS-PAGE gels. Left panel shows expression pattern before induction (Lane A) and 10 hours after induction (Lane B). Right panel shows purified <i>Rx</i>PAL protein (Lane C: 300 µg, Lane D: 200 µg, Lane E: 80 µg, Lane F: 40 µg, Lane G: 20 µg).</p
EGA/VGA to TV (PAL) conversion
No full textNowadays two of the most popular professional computer graphics cards for the PC (Personal Computer) are the EGA (Enhanced Graphics Adapter) and the VGA (Video Graphics Array) cards. These two graphics cards features relative high resolution and colors. Of different reasons it’s interesting to have the possibility to connect a PC with one of these graphics cards to a PAL (Phase Alternate Line) system television-set. However this is not possible because of different horizontal and vertical synchronization (the horizontal and vertical synchronization pulses dictate the scan rate of the display, i.e. computer monitor resp. television screen) of the EGA/VGA cards and the PAL system. In this report a way will be discussed in which this EGA/VGA to PAL conversion can be realized. By manipulating, i.e. refill with new values, the registers in charge of the synchronization on the EGA/VGA cards so that a PAL compatible signal can be achieved. This solution, based on register manipulation, gives a relative good picture on the television screen if alphanumeric characters are present on the computer. With graphics the conversion was not possible with this solution.The conclusion is that this method can be used only if alphanumeric characters are to be displayed on a television screen.Electrical Engineering, Mathematics and Computer ScienceTelecommunicatie- en Verkeersbegeleidingssysteme
A pal, Lue to Dear James (6 October 1962)
No full textSigned by A pal, Luehttps://egrove.olemiss.edu/mercorr_pro/1089/thumbnail.jp
SIMULATION RESULTS OF SELF SEEDING SCHEME IN PAL-XFEL
No full textAbstract There are two major undulator lines in Pohang Accelerator Laboratory XFEL (PAL XFEL), soft X-ray and hard X-ray. For the hard X-ray undulator line, selfseeding is the most promising approach to supply narrow bandwidth radiation to the users. The electron energy at hard X-ray undulator is 10 GeV and the central wavelength is 0.1 nm. We plan to provide the self-seeding option in the Phase I operation of PAL-XFEL. In this talk, the simulation results for the self-seeding scheme of hard X-ray undulator line in PAL XFEL will be presented
He\u27s Me Pal
No full text[Verse 1] I know a lad and when I feel bad He drives all me troubles away, When he’s your friend, he’s your to de end No matter what others may say; He don’t tell me how I ought to be, He likes me just as I am, So, when I gets blue, he’s the one I go to For his heart is as big as a ham.
[Chorus] He’s me pal, he’s me pal Dere ain’t nobody else I can see, I know he’s dead tough, but his love ain’t no bluff He’s share his last dollar with me; I’d rather have him with his fifteen a week Than be some old millionaire’s gal, He’s de best ever wuz, and I loves him becuz He’s me pal, he’s me pal.
[Verse 2] My pal is poor, but I am dead sure He’s better than most millionaires, I’m satisfied when he’s at me side Me joys and me troubles he shares; I’d give me arm to keep him from harm, He’d do de same thing for me, He’ll always be there, stormy weather or fair, That’s de reason I likes him, you see.
[Chorus]
[Verse 3] Friends may be few, friends may be true But I have one dear friend of old, Bless her dear heart, we will never part For she loves me better than gold, When she is near I have no fear, No harm to me can befall, She’s patient, resigned, she is loving and kind And I know she’s the best friend of all
[3rd Chorus] She’s me pal, she’s me pal She’s the very best friend that I know, Her herat’s full of love as the heavens above, She’s drives away sorrow and woe, I’ll try to repay all her kindness to me And if God be willing I shall, I have one friend that’s true, dear old mother that’s you, You’re me pal, you’re me pa
On Axiomatizations of PAL
No full textIn the literature, different axiomatizations of Public Announcement Logic (PAL) were proposed. Most of these axiomatizations share a 'core set' of the so-called reduction axioms. In particular, there is a composition axiom which stipulates how two consecutive announcements are composed into one. In this paper, by designing non-standard Kripke semantics for the language of PAL, we show that without the composition axiom the core set does not completely axiomatize PAL. In fact, most of the intuitive 'axioms' and rules we took for granted could not be derived from the core set. The non-standard semantics we proposed is of its own interest in modelling realistic agents. We show that with the help of different composition axioms we may axiomatize PAL w.r.t. such non-standard semantics.Computer Science, Artificial IntelligenceComputer Science, Theory & MethodsMathematics, AppliedEICPCI-S(ISTP)
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