3,024 research outputs found
Maturità fenolica: monitorare i processi evolutivi
No full textmaturità fenolica nel Sangiovese, effetto della parete cellulare delle bacche sulla estraibilità di antociani e tannini in Merlo
In-situ label-free optical detection of cells cultured in 3D microincubators
No full textIn this work, we show that high aspect-ratio silicon microstructures can play, at the same time, the roles of a cell-selective three-dimensional microincubator for cell culture and optical label-free transducer of cell morphology mapping. Silicon microincubators, integrating a periodic array of narrow (5-μm-wide), deeply etched (50-μm-deep) gaps separated by 3-μm-thick silicon walls, are fabricated by electrochemical micromachining (ECM) technology [1], and used for culturing several both epithelial and mesenchymal cell lines. Fluorescence microscopy analyses highlight that the microincubator shows cell-selective capabilities, being mostly cells with mesenchymal phenotype able to actively colonize the deeply etched gaps and grow attached to the vertical silicon walls [2]. The microincubator also features reflectivity spectral properties typical of one-dimensional (1D) photonic crystals (PhCs) structures in the near infrared range, with high reflectivity regions separated by deep reflectivity notches. According to 1DPhC optical properties, the presence of cells inside the gaps of the microincubator strongly affects the reflectivity signal, which can be measured in-situ with a fiber-optic setup orthogonally to the silicon wall surface (x-y plane). By spatially mapping the reflected power spectrum in the vertical x-y plane, it is thus possible to infer on the extension of cells growing into the microincubator attached to silicon walls. In particular, the intensity ratio between reflectivity maximum and minimum at two different wavelengths around 1.55 μm is closely related to the cell spreading on the silicon wall inside the deeply etched gaps of the microincubator. These results clearly envisage future in-situ label-free analyses of cellular activities involving changes in cell morphology and/or adhesion (e.g. apoptosis), in a three-dimensional environment.
[1] M. Bassu, S. Surdo, L. M. Strambini, G. Barillaro, Adv. Funct. Mat., 22 (2012), 1222-1228;
[2] F. Carpignano, G. Silva, S. Surdo, V. Leva, A. Montecucco, F Aredia, A. Scovassi, S. Merlo, G. Barillaro, G. Mazzini, Plos ONE 7 (2012) DOI: 10.1371/journal.pone.0048556
Maturità fenolica: monitorare i processi evolutivi
No full textmaturità fenolica nel Sangiovese, effetto della parete cellulare delle bacche sulla estraibilità di antociani e tannini in Merlo
La Convenzione sui diritti dell'infanzia in trent'anni di scuola italiana
No full textLa domanda alla quale si cercherà di rispondere con il presente contributo concerne significato e ricadute che l’istituzione di diritti specifici dei minori ha avuto in ambito scolastico. Atto giuridico di fondamentale importanza e dalla profonda valenza etica, capace di incidere sui costumi e sulla cultura, la Convenzione obbliga, a distanza di tre decenni, ad una riflessione in ordine alla complicata realizzazione che tale costrutto lessicale e giuridico ha avuto nella realtà sociale, culturale ma soprattutto educativa. Di qui la necessità di riflettere in merito all’attuazione dell’articolo 28 il quale rimanda non solo ad un insegnamento primario obbligatorio e gratuito per tutti ma anche alla concretizzazione di varie forme di insegnamento superiore atte a garantire la regolarità della frequenza e la diminuzione del tasso di abbandono della scuola. Il fine primario di favorire lo sviluppo della personalità nonché lo sviluppo delle facoltà e delle attitudini mentali e fisiche di ciascuno soggetto (art. 29) diventano i capisaldi di una educazione alla cittadinanza attiva e responsabile. Nella Convenzione troviamo allora sanciti i diritti alla conoscenza e all’istruzione, intesa quale materia prima dello sviluppo delle Nazioni, all’interno dei quali bambini e adolescenti, dismessi i panni di esclusivi destinatari del dovere di protezione dell’adulto, vestono quelli di cittadini in atto e in crescita partecipi, proprio perché detentori di diritti, delle comunità di appartenenza
Identification and Estimation of Stochastic Bargaining Models, Third Version
No full textStochastic sequential bargaining models (Merlo and Wilson (1995, 1998)) have found wide applications in different fields including political economy and macroeconomics due to their flexibility in explaining delays in reaching an agreement. This paper presents new results in nonparametric identification and estimation of such models under different data scenarios.Nonparametric identification, non-cooperative bargaining, stochastic sequential bargaining, rationalizable counterfactual outcomes
Identification and Estimation of Stochastic Bargaining Models, Fourth Version
No full textStochastic sequential bargaining models (Merlo and Wilson (1995, 1998)) have found wide applications in different fields including political economy and macroeconomics due to their flexibility in explaining delays in reaching an agreement. This paper presents new results in nonparametric identification and estimation of such models under different data scenarios.Nonparametric identification and estimation, non-cooperative bargaining, stochastic sequential bargaining, rationalizable counterfactual outcomes
An improved representation of gait curves ensemble average
No full textThe mean cycle plus/minus one or two standard deviations is widely used to represent the gait patterns of specific populations, as in the case of normality data. While this approach is correct when a wide sample is available, it might produce unreliable patterns when the sample size is reduced, the data distribution is not Gaussian and in presence of outliers. Moreover, it forces the band around the mean value to be symmetric, which can be not realistic when studying populations of patients. An alternative representation is proposed in this work, based on the use of the median curve with gray-scaled percentile bands
Federico I Barbarossa e i comuni nell'Archivio Storico Lombardo (dalla fondazione alla prima guerra mondiale)
No full textTuning of mechanical cough assistance in neuromuscular patients by optoelectroinc plethysmography
No full textMechanical Insufflation-Exsufflation (MI-E) is a therapy used by patients with neuromuscular disease and muscle weakness due to central nervous system injury. It decreases episodes of respiratory failure, particularly during upper-respiratory-tract infection, and provides greater success in weaning from mechanical ventilation than do conventional methods [1]. During MI-E it is difficult to assess the respiratory pattern in neuromuscular patients. Optoelectronic plethysmography (OEP) has proven to be a reliable alternative to invasive and/or voluntary manoeuvres to evaluate respiratory pattern [2]. The aim of the study was to determine the effects of a step-by-step increase in insufflation-exsufflation levels on the tolerance, the expired volume, the breathing pattern, and the respiratory function of a sample of patients affect by a neuromuscular disease
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