71 research outputs found
Caspase-3 is not essential for DNA fragmentation in MCF-7 cells during apoptosis induced by the pyrrolo-1,5-benzoxazepine, PBOX-6
No full textEffector caspases-3, -6 and -7 are responsible for producing the morphological features associated with apoptosis, such as DNA fragmentation. The present study demonstrates that a member of a novel series of pyrrolo-1,5-benzoxazepines, PBOX-6, induces apoptosis in MCF-7 cells, which lack caspase-3. Apoptosis was accompanied by DNA fragmentation and the activation of caspase-7, but not caspases-3 and -6. Inhibition of caspase-7 activity reduced the extent of apoptosis induced, indicating that activation of caspase-7 is involved in the mechanism by which PBOX-6 induces apoptosis in MCF-7 cells. This study suggests that caspase-3 is not necessarily essential for DNA fragmentation and the morphological changes associated with apoptosis. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved
Rick Bragg, 1997 Harry M. and Edel Ayers Lecture Series 1
No full textAuthor Rick Bragg was featured at the Harry M. and Edel Ayers Lecture Series held November 19, 1997 on the 11th floor of the Houston Cole Library at Jacksonville State University.https://digitalcommons.jsu.edu/lib_ac_histimg_1990/1381/thumbnail.jp
Rick Bragg, 1997 Harry M. and Edel Ayers Lecture Series 2
No full textAuthor Rick Bragg was featured at the Harry M. and Edel Ayers Lecture Series held November 19, 1997 on the 11th floor of the Houston Cole Library at Jacksonville State University. Shown Rick Bragg chats with Opal A. Lovett after giving his speech.https://digitalcommons.jsu.edu/lib_ac_histimg_1990/1382/thumbnail.jp
Protein Synthesis and Turnover
No full textUpon completion of transcription, the newly synthesized messenger RNA (mRNA) translocates out of the nucleus and into the cytoplasm where it gets translated into functional proteins. Decoding the mRNA into an amino acid sequence requires the concerted action of both RNA molecules, including transfer RNAs (tRNAs) and ribosomal RNAs (rRNAS), as well as many cytoplasmic proteins. This chapter outlines the molecular events that underlie the translation of mRNA into protein, highlighting the mechanisms that ensure high fidelity of these events. This chapter also discusses how newly synthesized proteins are targeted to their correct intracellular location, as well as the quality control mechanisms in place that ensure protein integrity inside the cell
Regulation of Gene Expression: Basic Mechanisms
No full textThe study of genes and the mechanism whereby the information they hold is converted into proteins and enzymes, hormones, and intracellular signaling molecules is the realm of molecular biology. Except for the erythrocyte, all cells in the body have the same DNA complement. One of the most fascinating aspects of this work is the study of the mechanisms that control differential gene expression, both in time and in place, and the consequences if these control mechanisms are disrupted.The goal of this chapter is to introduce the basic concepts involved in regulation of protein-coding genes and how these processes are involved in the causation of human disease. The basic mechanism of gene regulation will be described first, followed by a discussion of a specific gene regulation system to highlight various aspects of the basic mechanism. The chapter will end with a discussion of various ways in which the gene regulatory apparatus can be adapted to suit the needs of different tissues and situations.<br/
Polyphony and the anxiety of influence in the fiction of Henry James
Get PDFJames's fiction, especially in the Middle Phase, centres
on the figure of the artist and is characterized by, the two
interrelated aspects which previous criticism has largely
overlooked: the Bakhtinian 'polyphonic' -creation of
'author-thinkers'; and the conflict between ephebes and
precursors, for which Harold-Bloom's concept of 'the-anxiety of
influence' is the most illuminating model. Polyphony is the
narrative mode, and influence is the intra-artistic, theme.
These, as the Introduction to the thesis makes clear, are
rehearsed in James's inaugural novel, Roderick Hudson. Rowland
Mallet is an author-thinker, and his failure is caused by
authorial limitations. His monologism -is impaired by his
mistaking empathy for the authorial sympathy. Likewise,
Hudson's failure does not arise from a mercurial temperament,
but from a polyphonic shortcoming: not possessing the power of
fiction to contain the fiction of power in, his mentor. And the
relationships among the three artists - Gloriani, Hudson and
Singleton - perfectly exemplify the Bloomian-theme. It is these
two concepts, polyphony and influence, which are the major
preoccupation in the Middle Phase; as, the works chosen
demonstrate. These are a novella, a novel, and a number of
short stories all of which have been unjustifiably neglected.
Chapter One, on The Aspern Papers, argues that Tina Bordereau,
far from being, the artless victim seen by many critics,
actually challenges and defeats the narrator by the very form
of her narrative. Her 'realist' discourse undermines his
language of 'romance', and shows up its internal unstability.
Chapter Two is an extensive study of the critical reception of
The Tragic Muse. The most common areas of critical attention
have been its contemporary topicality, its relation to previous
novels on similar themes, and the possible genealogy of Gabriel
Nash. Those have all missed the core of the work. - Chapter Three
demonstrates how polyphony and the anxiety of influence make
the novel what it really is. Influence arises from the
juxtaposition of, and the wrestling between, artistic ephebes
and their precursors (Nick and Nash,, Miriam and Madame Carre).
The dialogic quality defined by Bakhtin is crucial to the
proper, and even-handed, characterization of all, the conflicts
in the novel. And since most of James's tales in the eighties
and nineties -are about 'masters - and acolytes, the anxiety of
influence remains central. Chapter Four is a study of 'The
Author of Beltraffiol' and 'The Lesson of the Master'. Again the
characters' manipulations are a crucial focus in a way that
G6rard Genette's terminology helps to illuminate. The fact that
the ephebe is the author-thinker emphasizes the inextricability
of the Bakhtinian and the Bloomian in James. Just as
polyphony offers a different focus for explicating the poetics
of James's fiction; so the ephebal conflict provides the basis
for a fresh perception of James's own artistic struggle
Rick Bragg Attends On the Brink, 1997 JSU Conference
No full textAuthor Rick Bragg was featured at the Harry M. and Edel Ayers Lecture Series held November 19, 1997 on the 11th floor of the Houston Cole Library at Jacksonville State University. Rick Bragg also attended On the Brink, a JSU conference of emerging Southern Writers. Shown from left are Harvey M. Jackson, Sibley Fleming, John McCormick, Rick Bragg, Deborah Smith, Jerry Ellis, Charles McNair, and Robert Coram.https://digitalcommons.jsu.edu/lib_ac_histimg_1990/1380/thumbnail.jp
From crops to clinic: the impact of dual azole use on antifungal resistance in Candida and Candida associated yeasts
No full textInvasive fungal infections caused by pathogenic yeasts are an escalating global health crisis that demands urgent attention within a One Health framework. This review critically examines mounting evidence that widespread agricultural azole fungicide use is a key driver accelerating antifungal resistance in pathogenic yeast. We dissect the shared molecular targets and resistance pathways that underpin dangerous cross-resistance between environmental fungicides and clinical azoles. Traditionally viewed as human commensals, we provide a comprehensive account of the evidenced environmental reservoirs of yeast pathogens, including agricultural soils, wastewater, and the food chain. Ecosystems burdened by persistent azole contamination that create hotspots for resistance evolution and amplification. With antifungal treatment options rapidly diminishing and resistant infections causing rising morbidity and mortality worldwide, we identify vulnerabilities in our shared environment and consider integrated surveillance, stewardship, and environmental interventions to help preserve the efficacy of life-saving antifungals and mitigate the growing threat of fungal disease.<br/
Synthesis of two novel thermally stable classes of polynorbornene with pendant aryl ether or ester chains.
No full textReactions of 5-norbornene-2-methanol with arene cyclopentadienyliron complexes led to the synthesis of two new classes of norbornene monomers with ether or ester bridges; ring-opening metathesis polymerization of these monomers using ruthenium-based catalysts gave rise to high molecular weight polymers displaying exceptional thermal stability
Yeast Epigenetics: The Inheritance of Histone Modification States
Get PDFSaccharomyces cerevisiae (budding yeast) and Schizosaccharomyces pombe (fission yeast) are two of the most recognized and well-studied model systems for epigenetic regulation and the inheritance of chromatin states. Their silent loci serve as a proxy for heterochromatic chromatin in higher eukaryotes, and as such both species have provided a wealth of information on the mechanisms behind the establishment and maintenance of epigenetic states, not only in yeast, but in higher eukaryotes. This review focuses specifically on the role of histone modifications in governing telomeric silencing in S. cerevisiae and centromeric silencing in S. pombe as examples of genetic loci that exemplify epigenetic inheritance. We discuss the recent advancements that for the first time provide a mechanistic understanding of how heterochromatin, dictated by histone modifications specifically, is preserved during S-phase. We also discuss the current state of our understanding of yeast nucleosome dynamics during DNA replication, an essential component in delineating the contribution of histone modifications to epigenetic inheritance.</p
- …
