1,720,967 research outputs found
Development of Limbal Epithelial Cell Carrier via Integration of Heterogeneous Collagen Matrices
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Aquatic flower-inspired cell culture platform with simplified medium exchange process for facilitating cell-surface interaction studies
No full textEstablishing fundamentals for regulating cell behavior with engineered physical environments, such as topography and stiffness, requires a large number of cell culture experiments. However, cell culture experiments in cell-surface interaction studies are generally labor-intensive and time-consuming due to many experimental tasks, such as multiple fabrication processes in sample preparation and repetitive medium exchange in cell culture. In this work, a novel aquatic flower-inspired cell culture platform (AFIP) is presented. AFIP aims to facilitate the experiments on the cell-surface interaction studies, especially the medium exchange process. AFIP was devised to capture and dispense cell culture medium based on interactions between an elastic polymer substrate and a liquid medium. Thus, the medium exchange can be performed easily and without the need of other instruments, such as a vacuum suction and pipette. An appropriate design window of AFIP, based on scaling analysis, was identified to provide a criterion for achieving stability in medium exchange as well as various surface characteristics of the petal substrates. The developed AFIP, with physically engineered petal substrates, was also verified to exchange medium reliably and repeatedly. A closed structure capturing the medium was sustained stably during cell culture experiments. NIH3T3 proliferation results also demonstrated that AFIP can be applied to the cell-surface interaction studies as an alternative to the conventional method.110Nsciescopu
Development of dense collagenous construct mimicking native corneal stroma based on collagen compression process
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A simple fabrication process for stepwise gradient wrinkle pattern with spatially-controlled wavelength based on sequential oxygen plasma treatment
No full textThough a gradient surface wrinkle pattern has drawn much interest recently, the previous efforts have been limited to the generation of continuous gradient patterns of surface wrinkle with gradually varying wavelengths. Here, we report a simple fabrication process for a stepwise gradient wrinkle pattern which has spatially-controlled discrete wavelengths in distinct regions with clear boundary on a polydimethylsiloxane (PDMS) substrate. The stepwise gradient wrinkle pattern is achieved based on the sequential treatment of oxygen plasma for the PDMS surface using a glass plate as an effective blocking mask for the plasma, resulting in the modulation of plasma dose on the specific regions of the PDMS surface. We fabricated a stepwise gradient wrinkle pattern on three regions of uniform feature size with 540, 840, and 1240 nm in wavelength and 60, 110, and 220 nm in depth. The utility of the stepwise gradient wrinkle pattern as a platform for screening global surface properties induced by a number of wrinkle patterns was evaluated by assessing the difference of anisotropic wetting behavior and transmittance depending on wrinkle feature sizes. This fabrication method can be easily implemented in microfluidic and optical applications investigating effects of various wrinkle groove patterns. (C) 2017 Elsevier B.V. All rights reserved.1131sciescopu
Development of Limbal Epithelial Cell Carrier via Integration of Heterogeneous Collagen Matrices
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Development of transplantable collagenous composite based on collagen compression process for treating limbal stem cell deficiency
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Fabrication of polystyrene-based multi-well screening platform for micrometer-scale surface topographies promoting stem cell functions
No full textThe study of surface topographic effect on stem cell function is getting attention these days due to its potential clinical applications such as regenerating injured tissues and drug screening. In this study, we suggest a simple and rapid fabrication method for a novel multi-well screening platform which contains a polystyrene (PS) bottom plate possessing different micrometer-scale surface topographies fabricated by the hot embossing process utilizing a polydimethylsiloxane (PDMS) mold. To obtain high replication quality with the reduced geometric deformation of surface topography, the processing condition for replication of the PS bottom plate was optimized through the design of experiments. The fabricated PS bottom plate is chemically bonded with a polymethyl methacrylate (PMMA). partition whose function is (1) isolating one type of surface topographies into an individual well and (2) preventing cross-contamination of soluble factors from a cell culture medium, resulting in a total fabrication time for one screening platform similar to 10 min. Given that the present platform is based on PS, which is the most widely used material in cell-based studies for the past decades, it is advantageous to compare the results from the present platform with the previously validated results performed in the conventional PS cell-culture wares. The effects of various micropillar arrays on the proliferation of human adipose-derived stem cells were examined using the present multi-well screening platform. Through those fabrication and stem cell-based experiments, it was shown that there is a possibility of considering the suggested fabrication method to be used for fabricating a standard screening platform for effective surface topography which promotes stem cell functions. (C) 2016 Elsevier B.V. All rights reserved.1111sciescopu
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