152,508 research outputs found
A probabilistic derivative measure based on the distribution of intensity difference
No full textIn this paper, we propose a novel derivative measure based on the probability of intensity difference that is defined by observed intensities and their true intensities. Because the true intensity cannot be estimated accurately only using two observed intensities, the probability is marginalized to consider an entire set of possible true values. The proposed measure not only considers intensity dependent noise effectively using a distribution of intensity difference, but also computes the relevant difference of two corresponding pixels that have different true intensities by extending the same intensity assumption in previous works. Using the proposed measure, the estimation result of image derivative for synthetic noisy signals is closer to the ground truth than most of previous measures. We apply the proposed measure for block matching and corner detection that compute intensity similarity in the temporal domain and image derivative in the spatial domain, respectively
Effect of doxycycline-regulated ERp57 expression on thrombopoietin productivity in recombinant Chinese hamster ovary cells
No full textEnhancing effect of low culture temperature on specific antibody productivity of recombinant Chinese hamster ovary cells: Clonal variation
No full textTo understand the different responses of recombinant Chinese hamster ovary (rCHO) cells to low culture temperature regarding specific productivity (q), 12 parental clones and their corresponding amplified clones producing a humanized antibody were cultivated at 32 and 37 degreesC. The specific growth rate of all clones, including both parental and amplified clones, decreased by 30-63% at 32 degreesC, compared to rates at 37degreesC. In contrast, their specific antibody productivity (q(Ab)) was significantly enhanced at 32 degreesC. Furthermore, the degree of q(Ab) enhancement at 32 degreesC varied a lot from 4- to 25-fold among the parental clones. At 32 degreesC, most of the amplified clones, regardless of methotrexate (MTX) levels, also showed enhanced q(Ab) but to a lesser extent than their parental clones. However, clone 14 amplified at 0.32 muM MTX (clone 14-0.32) and clone 20 amplified at 1 muM MTX (clone 20-1.00), unlike their parental clones, did not show enhanced q(Ab) at 32 degreesC. Thus, it was found that the enhancing effect of low culture temperature on q of rCHO cells depends on clones. Taken together, the results obtained here emphasize the importance of clonal selection for the successful application of low culture temperature to the enhanced foreign protein production in rCHO cells
CALOS : Camera And Laser for Odometry Sensing
No full textThis research is supported by
National Research Laboratory (NRL) program (No.
M1-0302-00-0064) of Ministry of Science and
Technology (MOST), and Agency for Defense
Development (ADD)
Effect of Doxycyclin-Regulated Chaperone Expression on Foreign Protein Productivity of Recombinant CHO Cells
No full text
Modeling and implementation of digital rights
No full textThe widespread use of the Internet raises issues regarding intellectual property. After content is downloaded, no further protection is provided on that content. DRM (Digital Rights Management) technologies were developed to ensure the protection of digital information. In this paper, we explore the issues associated with content distribution and digital rights management. We develop a modified Imprimatur model based on a multi-level content distribution model and, based on those two models, we present the formal model of a new DRM system and identify the properties that a DRM system should have. We design a prototype DRM system that distributes due royalties among participants, enforces content usage rules, and distributes content keys securely in the content distribution channel. (C) 2003 Elsevier Inc. All rights reserved
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