1,957 research outputs found

    Microbial community structure in full scale anaerobic mono-and co-digesters treating food waste and animal waste

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    Five mesophilic full-scale anaerobic digesters treating food waste (FW-digester), animal waste (AW-digester), and co-substrate of food waste and animal waste (CO-digesters) were monitored identify bacterial and archaeal communities and to quantify the effect of substrate characteristics on them, and to identify 'core' microorganism. The substrate characteristics and microbial communities of the FW-digester, AW-digester, and CO-digesters were statistically different. Organic concentration and [Na+] were identified as major variations that effect microbial community. Methanogen community was more diverse in AW-digester than in FW-digester. Methanogen community in CO-digester was as diverse as in AW-digester, and the most dominant species was Methanoculleus bourgensis same as in FW-digester. Twenty-one bacterial genera and four methanogen species were found in all digesters as a consequence of their metabolic versatility to degrade organic and inhibitor compounds. The results implied that these core microorganisms may contribute to maintaining a stable microbial ecosystem.11Nsciescopu

    Influence of temperature and sliding speed on the subsurface microstructure evolution of EN AW-6060 under sticking friction conditions

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    This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in AIP Conference Proceedings 1896, 140012 (2017) and may be found at https://doi.org/10.1063/1.5008168.The microstructure evolution of the friction boundary layer of the aluminum alloy EN AW-6060 was investigated. Sticking friction tests at different temperatures and sliding speeds were carried out. A severe deformation below the friction surface was observed by means of LOM and EBSD mapping. Thus, the thickness variation and the grain structure of the high deformation zone could be described. Fibrous structure was observed at 300 °C and 400 °C, while equiaxed grains with high misorientation angle (>15°) were generated at higher temperatures. Additionally, abnormal grain growth and coarse grains were detected at high sliding speeds (10 mm/s, 42 mm/s) at 450°C and 500 °C respectively

    Ecology of A.carbonarius and ochratoxin A production in vine fruits and control in the production chain

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    This study examined black aspergilli, especially A. carbonarius and A. niger and ochratoxin A (OTA) contamination of grapes, during drying and industrial processing of dried vine fruits. This was complemented by studies on potential control using preservatives and physical factors such as modified atmospheres. Fungal population kinetics were determined in relation to grapes at harvest, and during drying at three different altitudes (sea level: 0-200 m; medium level: 250-500 m; high level: >500 m) in two seasons. At pre-harvest, A. niger aggregate species were the predominant fungal species while A. carbonarius was occasionally isolated, in both years studied. Both altitude and bunch position affected black aspergilli population dynamics. Overall, they were increased during drying. However, both black aspergilli groups were mostly isolated, at low and medium altitudes (<500 m). OTA contamination was influenced by bunch position, although altitude did not significantly influenced amounts. The fungal biodiversity was decreased during sun-drying of sultanas. The widest diversity of species occurred at the sea level. However, A. niger aggregate, were dominant during drying. Time of drying and altitude significantly influenced fungal loads of black aspergilli. In contrast, OTA production (ca 0.001 - 0.0025 μg g - 1 ) was not significantly influenced by altitude and drying time. Shannon Index of Biodiversity (H), for pre-harvest and pos-harvest studies, was determined for the first time. A. niger aggregate (ca 5.0 Log10 CFUs g -1 ) was predominant during industrial processing, while A. carbonarius was only isolated at low levels (1.5-2.0 Log10 CFUs g -1 ). Heat treatment (up to 90 o C) appeared to be the key-procedure for the elimination of fungal populations. In the contrary, SO2 treatment did not statistically alter fungal population dynamics. OTA contamination was not significantly affected by industrial processing. In vitro studies conducted on both White Grape Juice Medium (WGJM) and in sultanas with strains of A. carbonarius originated from Cretan sultanas and compared with a strain isolated from Italian wine grapes. They examined the impact of sodium metabisulphite (NaMBS), elevated CO2 (up to 50%) concentrations and aw levels, on black aspergilli spore germination, growth and OTA production. Moreover, fungal interactions in vitro and in situ were also investigated. In general, spore germination occurred over a wide range of sodium metabisulphite concentrations, although germ tube extension was significantly controlled. At ≥ 750 mg L -1 NaMBS, no spore germination was observed while both mycelial growth and OTA production were completely inhibited. Medium concentrations of NaMBS (≤ 250 mg L -1 ) enabled optimum spore germination, growth and OTA production (x 0.965 aw). The efficacy of controlled atmospheres x aw showed that there was very little inhibitory effect on spore germination. However, both germ tube extension and fungal growth were inhibited by 50% CO2. After 10 days, growth was not as effectively controlled. Aw had a bigger effect on OTA production than modified atmospheres. In situ experiments on sultanas confirmed these results. Competition and dominance of A. carbonarius over other fungal species showed that aw and temperature influenced Indices of Dominance and OTA production. In vitro and in situ, OTA production by A. carbonarius was significantly influenced by the fungal competitor used

    Highly responsive near-infrared photodetector with low dark current using graphene/germanium Schottky junction with Al2O3 interfacial layer

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    The performance of a graphene/Ge Schottky junction near-infrared photodetector is significantly enhanced by inserting a thin Al2O3 interfacial layer between graphene and Ge. Dark current is reduced by two orders of magnitudes, and the specific detectivity is improved to 1.9 x 10(10) cm . Hz(1/2)W(-1). The responsivity is improved to 1.2 AW(-1) with an interfacial layer from 0.5 AW(-1) of the reference devices. The normalized photo-todark current ratio is improved to 4.3 x 10(7) W-1 at a wavelength of 1550 nm, which is 10-100 times higher than those of other Ge photodetectors.11Ysciescopu
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