1,673 research outputs found
Increased production of Bacterial Cellulose by Metabolic engineering of Komagataeibacter xylinus with Newly Developed synthetic parts
No full textAuthor Rights and Copyright Management
No full textPresentation and slides delivered during Open Access Week 2010 by Peggy Hoon, copyright education specialist for the University of North Carolina at Charlotte. View the video of Hoon's talk, or view the slides
A novel method to detect mutation in DNA by utilizing exponential amplification reaction triggered by the CRISPR-Cas9 system
No full textWe, herein, describe a novel method to detect mutation in DNA by utilizing exponential amplification reaction (EXPAR) triggered by clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9, called CRISPR-EXPAR. The CRISPR system consisting of two Cas9/sgRNA complexes was designed to cut out a specific mutation region within the target DNA, which would consequently promote EXPAR by continuously repeated extension and nicking reactions. As a consequence, a large number of final EXPAR products, which can be monitored through duplex-specific fluorescent staining, are produced. Based on this design principle, we successfully identified a model target mutation within the human epidermal growth factor receptor 2 (HER2) gene down to 437 aM with excellent specificity. The practical capability of this method was verified by reliably identifying the target mutation directly from the genomic DNA (gDNA) extracted from the lung cancer cell line, NCI-H1781 (H1781), and its universal applicability was further confirmed by identifying another EFGF L858R mutation. This technique could serve as a new isothermal platform to identify various mutations by rationally redesigning single guide RNA (sgRNA) according to the target mutation site.
Author Rights and Copyright Management
No full textPresentation and slides delivered during Open Access Week 2010 by Peggy Hoon copyright education specialist for the University of North Carolina at Charlotte. View the video of Hoon's talk or view the slides
The Treatment of Solar Lentigo using Dr. Hoon Hur's Optimal Melanocytic Suicide-2 Parameter with a High fluence 1064nm Nd:YAG Laser without Postinflammatory Hyperpigmentation
No full textFunMaths / Teh Sok Hoon
No full textOver the past two decades electronic game have become ingrained in our culture Children's fixation with these games initially alarmed parents and educators, but educational researchers soon questioned whether the motivation to play could be tapped and harnessed for educational purposes. A number of educational electronic games have been developed and theory success has been mixed. Thus, I have chosen my thesis title as " Interactive Multimedia Educational Game" which we have name it as Kids Heaven and I will develop an educational gamer for mathematics which I name it as FunMaths while my partner will be doing about Science, FunScience. This Game was created in CD-Rom based and will be suitable for students in primary school especially age between 7 and 12, parents and teacher and will only include questions based on Mathematics operations for not more than three digits. Users will be able to choose the level of difficulties. One of the objectives of this game is to help teachers in teaching mathematics in English and to help the students to understand more about the subject. The system implements the concepts of multimedia, which integrates text, graphics, animations and audio into the game display to make the game more attractive especially for the kids. Macromedia Author ware 6, Macromedia Flash MX, Adobe Photoshop 6.0 and some other tools was used to develop the interactive game. Finally, I hope the "Multimedia Interactive Educational Game" can serve to enable players enjoy themselves throughout the playing and learning process, and to achieve the objective specified before the system is develop
Somebody is Looking Back At Me
No full textSomebody is Looking Back At Me, a new play by Jesse Jae Hoon, is a time-jumping fever dream satire following bestselling Asian American author Olivia, who returns to a gentrifying Chinatown. As her new successful friends transform the neighborhood, she must confront the district’s troubled past and her own allegiances
Cancer-Associated Fibroblast-Induced Resistance to Chemotherapy and Radiotherapy in Gastrointestinal Cancers
No full textIn the past few decades, the role of cancer-associated fibroblasts (CAFs) in resistance to therapies for gastrointestinal (GI) cancers has emerged. Clinical studies focusing on GI cancers have revealed that the high expression of CAF-related molecules within tumors is significantly correlated with unfavorable therapeutic outcomes; however, the exact mechanisms whereby CAFs enhance resistance to chemotherapy and radiotherapy in GI cancers remain unclear. The cells of origin of CAFs in GI cancers include normal resident fibroblasts, mesenchymal stem cells, endothelial cells, pericytes, and even epithelial cells. CAFs accumulated within GI cancers produce cytokines, chemokines, and growth factors involved in resistance to therapies. CAF-derived exosomes can be engaged in stroma-related resistance to treatments, and several non-coding RNAs, such as miR-92a, miR-106b, CCAL, and H19, are present in CAF-derived exosomes and transferred to GI cancer cells. The CAF-induced desmoplastic reaction interferes with drug delivery to GI cancer cells, evoking resistance to chemotherapy. However, due to the heterogeneity of CAFs in GI cancers, identifying the exact mechanism underlying CAF-induced resistance may be difficult. Recent advancements in single-cell “omics” technologies could offer clues for revealing the specific subtypes and biomarkers related to resistance
Induced Norm-Based Analysis for Computed Torque Control of Robot Systems
No full textThis paper is concerned with providing a new quantitative analysis method of computed torque control of robot systems by using three types of induced norm. This paper first considers design of a computed torque controller to achieve a trajectory tracking performance of a robot manipulator. Taking into account of the effects of unknown elements on the tracking performance, this paper next divides the unknown elements into model uncertainty and disturbance, and introduces various closed-loop representations of robot systems consisting of robot manipulators together with computed torque controllers and unknown elements. This paper further derives a readily applicable robust stability condition for the model uncertainty by using two types of induced norm from L-2 to L-2 and from L-infinity to L-infinity. Regarding a performance analysis for the disturbance, this paper also proposes to take the induced norm from L-2 to L-infinity, by which the relation between the maximum tracking errors caused by the disturbance and the corresponding parameters of the computed torque controllers are dealt with. Finally, this paper gives some experiments to validate the effectiveness of the performance analysis methods based on the L-infinity/L-2-induced norm.11Ysciescopu
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