7,532 research outputs found
Kerouac's crooked road development of a fiction
No full textNow available for the first time in paperback, with a new foreword by Ann Charters, here is Tim Hunt's incisive look into Jack Kerouac's creative process and achievement. Debunking much of the mythology about Kerouac, Hunt shows the author of On the Road and Visions of Cody working out the literary strategies that link him to Herman Melville, Mark Twain, and other canonical American novelists. This is an essential book for anyone interested in Beat culture and Kerouac's conscious literary artistry
Do dolphins benefit from nonlinear mathematics when processing their sonar returns?
No full textAn interview with author Tim Leighton about the paper
Contributions of Dna2 and the Tim/Tipin Complex to Genomic Stability
No full textThis thesis describes the essential roles of Dna2 and the Tim/Tipin complex in the maintenance of genomic stability. Dna2 participates in DNA replication and double-strand break repair by homologous recombination. Meanwhile, the Tim/Tipin complex is required for efficient checkpoint activation upon replication stress, which can be caused by stalled DNA replication forks.
While yeast genetics and experiments with purified proteins have revealed much about yeast Dna2, we chose to pursue characterization of metazoan Dna2 using Xenopus cell-free extracts. We show that binding of Dna2 to origins of replication is dependent upon formation of pre-replication complexes but independent of CDK2 activity. Upon initiation of DNA replication, Dna2 travels with replication forks. Physical interactions with Mcm10 and And-1, proteins involved in lagging strand DNA replication, are indicative of a role in replication of the lagging strand; this result is consistent with genetic results in yeast and in vitro biochemical experiments.
Dna2 also participates in the response to double-strand breaks and accumulates on chromatin containing double-strand breaks. We show that Dna2 binds to free DNA ends after the Mre11-Rad50-Nbs1 complex and ATM, but before RPA. Dna2-depleted extracts exhibit delayed processing of DNA ends, indicating that other nucleases do not easily compensate for the lack of Dna2. Consistent with genetic results in yeast, we find that the Mre11-Rad50-Nbs1 protein complex is essential for the processing of free DNA ends, but inhibition of Mre11 nuclease activity only slows processing. This observation indicates that other nucleases, possibly Dna2, can compensate for loss of Mre11 nuclease activity. Despite the role of Dna2 in double-strand break processing, Dna2 is not required for checkpoint activation.
Timeless (Tim) and Tipin participate in the checkpoint response to stalled replication forks. We demonstrate here that Tim and Tipin form a complex, associate with chromatin in S phase, and physically interact with many proteins at the replication fork. Human cells lacking the Tim/Tipin complex do not exhibit robust checkpoint activation in response to stalled replication forks. Finally, we show that Tipin is also a target of both the ATR and Cdc7 kinases, which respond to stalled replication forks.</p
Opportunities for linking young surveyors across professional surveying member organisations and FIG
No full textTim Di Muzio on 'Sabotage'
No full textIn a series of essays published in 2013 and 2014 on capitaspower.com, political economist Tim Di Muzio explored the concept of ‘sabotage’ as it applies to capitalist power. I recently rediscovered these essays and was so impressed by them that I have reposted them here as a single piece.
About the author: Tim Di Muzio is a researcher at the University of Wollongong. He is the author of numerous books, including Debt as power, Carbon capitalism, and The 1% and the Rest of us
Evaluating Citebase, an open access Web-based citation-ranked search and impact discovery service
No full textCitebase is a new citation-ranked search and impact discovery service that measures citations of scholarly research papers which are openly accessible on the Web, i.e. papers that are assessable continuously online. Other services, such as ResearchIndex, have emerged in recent years to offer citation indexing of Web research papers. In the first detailed user evaluation of an open access Web citation indexing service, Citebase has been evaluated by nearly 200 users from different backgrounds. The paper details the procedures used in the evaluation, and analyses the results of this study, which took place between June and October 2002. It was found that within the scope of its primary components, the search interface and services available from its rich bibliographic records, Citebase can be used simply and reliably for the purpose intended, and that it compares favourably with other bibliographic services. It is shown tasks can be accomplished efficiently with Citebase regardless of the background of the user. More data need to be collected and the process refined before it is as reliable for measuring citation impact of indexed papers. Better explanations and guidance are required for first-time users. Coverage is seen as a limiting factor, even though Citebase indexes over 200,000 papers from arXiv. Non-physicists were frustrated at the lack of papers from other sciences. The principle of citation searching of open access archives has thus been demonstrated and need not be restricted to current users. Since the evaluation, Citebase has become a featured service of the ArXiv physics eprint archives
1996-1997 Tim Gautreaux
No full textTim Gautreaux is the author of three novels and two earlier short story collections. His work has appeared in The New Yorker, The Best American Short Stories, The Atlantic, Harper’s, and GQ. After teaching for thirty years at Southeastern Louisiana University, he now lives, with his wife, in Chattanooga, Tennessee. (Photo credit: Randy Bergeron)https://egrove.olemiss.edu/grisham_res/1023/thumbnail.jp
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Conclusions and reflections: retrofitting cities for tomorrow’s world
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