5 research outputs found
Hawthorne’s Unsympathetic Gaze: Unmasking Hester Prynne in the Eyes of Nathaniel Hawthorne
A lot of attention is paid to how Nathaniel Hawthorne treats Hester Prynne, who is the main character in "The Scarlet Letter." It's important for us to understand why Hawthorne's look is so cold and how the story's lack of care is built into the story itself. Hawthorne, who wrote Hester's story, makes it hard to tell the difference between how people really feel and how they show it to the public. The author's lack of care and mental distance seems to be what's wrong with the character. We read a lot of academic writing to help us figure out how this relationship works. We can learn a lot about how the author thinks about his work as it changes over time from these different points of view. By putting together different points of view, we want to show how Hawthorne's cold gaze affects the story and how it changes how readers see Hester. We want to give you a fuller picture of Hawthorne's troubled relationship with Hester Prynne by looking closely at different pieces of writing and critical analysis. By using different academic points of view in this way, we can better understand how complicated the relationship is between the author and the character in "The Scarlet Letter." This study looks at the idea that Hawthorne looked at Hester with a cold gaze. This helps us understand how complicated the book is and what the author's lack of emotion means in a bigger sense
Synthesis, and In Vitro and In Silico α-Glucosidase Inhibitory Studies of 5-Chloro-2-Aryl Benzo[d]thiazoles
Atomic force microscopic imaging of Acanthamoeba castellanii and Balamuthia mandrillaris trophozoites and cysts
© 2014 The Author(s) Journal of Eukaryotic Microbiology © 2014 International Society of Protistologists.Light microscopy and electron microscopy have been successfully used in the study of microbes, as well as free-living protists. Unlike light microscopy, which enables us to observe living organisms or the electron microscope which provides a two-dimensional image, atomic force microscopy provides a three-dimensional surface profile. Here, we observed two free-living amoebae, Acanthamoeba castellanii and Balamuthia mandrillaris under the phase contrast inverted microscope, transmission electron microscope and atomic force microscope. Although light microscopy was of lower magnification, it revealed functional biology of live amoebae such as motility and osmoregulation using contractile vacuoles of the trophozoite stage, but it is of limited value in defining the cyst stage. In contrast, transmission electron microscopy showed significantly greater magnification and resolution to reveal the ultra-structural features of trophozoites and cysts including intracellular organelles and cyst wall characteristics but it only produced a snapshot in time of a dead amoeba cell. Atomic force microscopy produced three-dimensional images providing detailed topographic description of shape and surface, phase imaging measuring boundary stiffness, and amplitude measurements including width, height and length of A. castellanii and B. mandrillaris trophozoites and cysts. These results demonstrate the importance of the application of various microscopic methods in the biological and structural characterization of the whole cell, ultra-structural features, as well as surface components and cytoskeleton of protist pathogens
