267,026 research outputs found
EFFECTS OF THE KARLOVITZ NUMBER ON THE EVOLUTION OF THE FLAME SURFACE DENSITY IN TURBULENT PREMIXED FLAMES
No full textDirect numerical simulation (DNS) is conducted to investigate the effects of the Karlovitz number (Ka) on displacement speed and consequent evolution of flame surface density (FSD). Parametric study is performed for the Ka between 2 and 10 in the thin reaction zone regime with independent variation of laminar flame speed and turbulent intensity. Previous study showed the effect of the turbulent intensity without noticeable influence of the Ka lower than 2.4 [I. Han, K.Y. Huh, Combust. Flame 152 (2008) 194-205]. A higher Ka involves a lower displacement speed on the positive curvature side primarily due to the influence on the normal diffusion component. It leads to a negative curvature term to act as a sink for FSD throughout a flame brush. The maximum FSD increases with increasing turbulent intensity, while a higher Ka leads to an asymmetric profile of FSD due to suppressed production at the leading edge. A higher Ka decreases total flame area and turbulent burning velocity as well, while a limiting behavior is shown for low Da cases. (C) 2009 The Combustion Institute. Published by Elsevier Inc. All rights reserved.X1131sciescopu
I lavori di June Huh
No full textUna delle quattro medaglie Fields assegnate nel 2022 è stata vinta dal matematico June Huh, per i suoi contributi altamente innovativi alla combinatoria. I lavori di June Huh, oltre a risolvere brillantemente varie congetture aperte da molto tempo, creano un nuovo ponte tra la combinatoria e settori della matematica in apparenza molto distanti quali la topologia e la geometria algebrica. In questo articolo ci proponiamo di dare una introduzione il più possibile elementare alla combinatoria e di spiegare alcuni tra i principali risultati di Huh dando anche un'idea dell'originalità dei suoi metodi.One of the four Fields medals assigned in 2022 has been awarded to the mathematician June Huh for his highly innovative contributions to combinatorics. Huh's papers, beside brilliantly solving several longstanding conjectures, build a new bridge between combinatorics and seemingly unrelated fields of mathematics such as topology and algebraic geometry. The purpose of this paper is to give an introduction, as elementary as possible, to combinatorics and explain some of Huh's main results, as well as giving an idea of the innovativeness of his methods
<i>In vivo</i> effect of bortezomib and ΔBtz on Huh-7 xeonograft nude mice.
No full text<p>A, tumor growth curves of Huh-7. Points, mean (n = 6); bars, SE. *<i>P</i><0.05; ** <i>P</i><0.01. B, western blot analysis of CIP2A, P-Akt, Akt1 and LC-3 in Huh-7 tumors. C, immunoblots were scanned by a UVP BioSpectrum AC image system and quantitated using VisionWork LS software to determine the ratio of the level of CIP2A to actin. <i>Columns</i>, mean; <i>bars</i>, SD (n = 3, *<i>P</i><0.05). D, summary of the mode of actions of bortezomib.</p
DV1 infection of HUH-7 and shRIG-I cells.
No full text<p>(A) Whole cell lysate from DV1-infected cells were subjected to Western blot analysis and probed for the antibodies indicated and visualized by enhanced chemiluminescence. The nitrocellulose membranes were then reprobed for β-actin (loading control). (B) Qualitative RT-PCR for MDA5 mRNA level in DV1-infected cells from different time points. Total RNA was isolated and subjected to RT-PCR analysis. GAPDH was used as a control for equal RNA templates. (C) Uninfected shRIG-I and HUH-7 cells were stimulated with synthetic polyI:C. Cell lysates were assessed after 24 h of stimulation by Western blot for MDA5. β-actin was used as a control for equal loading of cell lysates. (D) Real-time RT-PCR analysis of MDA5 expression in mock and DV1-infected cells. Total RNA was isolated, used for cDNA preparation and subjected to real-time RT-PCR analysis. Bar histograms show the average difference in gene expression between mock and DV1-infected cells based on at least two independent experiments. (E) siRNA silencing technique was used to silence RIG-I gene. In HUH-7 cells, and not in A549 cells, RIG-I silencing co-induced silencing of MDA5 gene. (F) A549 cells were transfected with siRNA for EGFP, RIG-I and/or MDA5. Whole cell lysate from mock and DV1-infected A549 cells were subjected to immunoblotting (IB) and RT-PCR analysis. Results show that DV1 infection is significantly higher in cell line deficient for both RIG-I and MDA5. (G) Whole cell lysate from HUH-7 and shRIG-I cells infected with either wild type or UV-treated DV1 virus were subjected to immunoblotting (IB) and RT-PCR analysis. DV1 was UV-inactivated by exposing the virus to a UV-lamp (wavelength, 254 nm) at a distance of 5 cm for 1 hour. Results show that UV-treated DV1 infection did not activate RIG-I and MDA5. (H) Specificity of monoclonal NS3 antibody. Myc-tagged DV1 NS1 and NS3 constructs were transfected into HUH-7 cells. Cell lysates were harvested 24 h later and used to test specificity of monoclonal NS3 antibody. DV1-infected HUH-7 cell lysate was used as a control. Anti-NS3 antibody was found to be specific for DV1 NS3 protein.</p
<i>MIR376A</i> overexpression blocked autophagy in Huh-7 cells.
No full text<p>(A) <i>MIR376A</i> blocked GFP-LC3 dot formation under starvation condition. (B) Quantitative analysis of experiments in A (mean ± S.D. of independent experiments, n = 3, ***p<0,01). (C) Overexpression of <i>MIR376A</i> resulted in decreased autophagic flux in Huh-7 cells. Starvation-induced conversion of LC3-I to LC3-II was analyzed. Tests were performed in the presence or absence of E64d (10 µg/ml) and Pepstatin A (10 µg/ml) (E+P). LC3-II/LC3-I densitometric ratios were marked. ACTB was used as a loading control.</p
Infectivity titers in culture medium and cells of HuH-7T1 and Huh-7.5.1 transfected with JFH-1 RNA.
No full text*<p><i>P</i><0.05 as compared with Huh-7.5.1.</p
Mr. Froggie went a courtin’ he did ride—uh, huh.
No full textvoiceCollected by John Cuffman
Sung by Mrs. Margaret Jones Fayetteville, Arkansas December 14, 1958
and Mike Caldwell For Mary Celestia Parler Transcribed by John Cuffman
and Mike Caldwell
Reel 294, Item 11
Mr. Froggie Went A Courtin'
Mr. Froggie went a courtin’ he did ride—uh, huh.
Mr. Froggie went a courtin’ he did ride A sword and a pistol by his side—uh, huh.
He came to the door of Mousie’s hall—uh, huh.
He came to the door of Mousie’s hall
He gave a loud knock and he gave a loud call—uh, huh.
"Pray lady Mouse are you within—uh, huh Pray lady Mouse are you within?"
"O yes, kind sir I sit and spin--uh, huh.
He took lady Mouse upon his knee—uh, huh
He took lady Mouse upon his knee
and said lady Mouse will you marry me—uh, huh?
Lady Mouse blushed and hung down her head—uh, huh Lady Mouse blushed and hung down her head and said, "You’ll have to ask Uncle Ned
Old Uncle Ned laughed and shook his fat side—uh, huh Old Uncle Ned laughed and shook his fat side To think that his niece would be a bride—uh, huh.
Where shall the wedding supper be? uh, huh Where shall the wedding supper be?
Away down yonder in a hollow tree—uh, huh.
What shall the wedding supper be? uh, huh What shall the wedding supper be?
Three blue beans and a blackeyed pea—uh, huh.
The first came in was a big black bug—uh, huh
The first came in was a big black bug
Who danced a jig with the whiskey jug—uh, huh.
The next came in was a hopping flea—uh, huh The next came in was a hopping flea A bow and fiddle upon his knee—uh, huh.Funding for digitization provided by the Arkansas Humanities Council and the Happy Hollow Foundation
Froggy went a-courting, he did ride, uh-huh,
No full textvoiceCollected by Max Hunter (H-5 ) Sung by J. W. Breazeal
For Mary C. Parler Springfield, Missouri
Transcribed by Frances Majors April 27, 1958
Reel 247, Item 13
Froggy Went A-Courting
Froggy went a-courting, he did ride, uh-huh,
Froggy went-a-courting, he did ride,
Sword and pistol by his side, uh-huh.
He rode till he came to the master's hall, uh-huh,
He rode till he came to the master's hall,
There he did both knock and call, uh-huh.
Took Miss Margy on his knee, uh-huh,
Took Miss Margy on his knee,
Says to her, Will you have me? uh-huh.
Old Uncle Rat he's gone from home, uh-huh,
Old Uncle Rat he's gone from home,
And I can't tell you till he's done come, uh-huh.
Uncle Rat laughed and shook his fat sides, uh-huh,
Uncle Rat laughed and shook his fat sides,
To think his niece would be a frog's bride, uh-huh.
Old Uncle Rat he galloped off to town, uh-huh,
Old Uncle Rat he galloped off to town
To buy his niece a wedding gown, uh-huh.
Where shall the wedding supper be? uh-huh,
Where shall the wedding supper be?
Way down yonder in a hallow tree, uh-huh.
What shall the wedding supper be? uh-huh
What shall the wedding supper be?
Two black beans and a blue-eyed pea, uh-huh.
First came in was an old black bug, uh-huh,
First came in was an old black bug,
He had his whiskey in his jug, uh-huh.
The next come in was a little black flea, uh-huh,
The next come in was a little black flea,
Had his fiddle on his knee, uh-huh.
Froggy Went-A-Courting (Cont'd)
Reel 247, Item 13 (Cont'd)
The next came in was an old sly cat, uh-huh,
The next came in was an old sly cat,
She says I'll put a stop to that, uh-huh.
Then Miss Mousy started up the wall, uh-huh,
Then Miss Mousy started up the wall,
Her foot slipped and she did fall, uh-huh.
The frog was left a widder then, uh-huh,
The frog was left a widder then,
Swore he never would marry again, uh-huh.
Started swimming across the brook, uh-huh,
Started swimming across the brook,
And an old black snake took him down his crook, uh-huh.
You may lay this all upon the shelf, uh-huh,
You may lay this all upon the shelf,
And if you want e'er nother one you must sing it yourself, uh-huh.Funding for digitization provided by the Arkansas Humanities Council and the Happy Hollow Foundation
Rapid induction of <i>XBP1</i> mRNA splicing by GGA treatment in HuH-7 cells.
No full text<p>Quantitative RT-PCR primers discriminating the spliced and unspliced forms of <i>XBP1</i> mRNA were designed and their nucleotide sequences are shown in <b>A</b>. Total mRNA was extracted from HuH-7 cells and RT-qPCR was performed using either these <i>XBP1u</i> or <i>XBP1s</i> primers. Following qPCR (45 cycles), products of <i>XBP1u</i> or <i>XBP1s</i> amplification were diluted by 10 or 50 fold, respectively, prior to electrophoresis by E-Gel 48 (4% agarose) to validate the discriminating primers (<b>B</b>). HuH-7 cells were treated with 10 (open circle) or 20 μM GGA (closed circle) for 0, 1, 2, 4, 8, and 24 h. Total mRNA was extracted to measure the cellular levels of <i>XBP1u</i> (<b>C</b>) and <i>XBP1s</i> (<b>D</b>) mRNA by quantitative reverse-transcription (RT)-PCR in duplicate. Each point represents the mean ± SD (n = 3). HuH-7 cells were treated with GGA (0–20 μM) for 1 h, and total mRNA was extracted to estimate the cellular levels of <i>XBP1u</i> (<b>E</b>) and <i>XBP1s</i> (<b>F</b>) mRNA by RT-qPCR. Each point represents the mean ± SE of seven independent experiments. Asterisks (*, **, ***) indicate statistically significant difference from a control sample at 0 h with p value of < 0.05, 0.01, 0.001, respectively, as determined by ANOVA followed by post hoc multiple comparison test. PLC/PRF/5 (<b>G</b>), HepG2 (<b>H</b>) or Hep3B (<b>I</b>) cells were treated with 20 μM GGA for 0, 0.5, 1, 2, 4, 8, and 24 h, and total mRNA was extracted to analyze XBP1u and XBP1s mRNA expression by RT-qPCR. Closed and open circles indicate the relative abundance of XBP1u and XBP1s mRNA, respectively.</p
<i>HAMP</i> mRNA expression in HepG2 and Huh-7 cells treated with different patients sera.
No full text<p>Results are expressed as 2<sup>−ΔΔCt</sup>, as median (range).</p><p>ND: not done.</p><p>HepG2 <i>HAMP</i> mRNA: THAL vs all (p<0.01); <i>HFE</i>-HH vs C-Donors (p<0.05); C-NAFLD vs C-Donors (p<0.01); C-NAFLD vs DIOS (p<0.02);</p><p>Huh-7 <i>HAMP</i> mRNA: THAL vs all (p<0.01); <i>HFE</i>-HH vs C-Donors (p<0.01).</p
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