1,725,530 research outputs found
hUC-MSC do not induce any biological effects on control mice treated with endotracheal saline.
No full textHistology (A-B), collagen content (C-D) and macrophage infiltration (E-H) of mouse lungs 21 days after sterile saline endotracheal injection followed by hUC-MSC intravenous infusion. Lung sections obtained from C57BL/6 mice (n = 8 per group) that received endotracheal sterile saline only (saline) or endotracheal sterile saline followed by intravenous hUC-MSC (saline+hUC-MSC) were stained with H&E (A-B), Picrosirius Red (C-D), anti- galectin-3 (E-F) and anti-arginase I (G-H) antibodies. Representative microscopic images (10× or 40× magnification) of three independent experiments are shown. Quantitative real-time PCR gene expression analysis of Col1A1, TGFβ and αSMA (I), and IL-1β, IL-2, IL-6 and IL-10 (J) in whole lung mRNA obtained at day 21 from C57BL/6 mice receiving the aforementioned treatments. Results are expressed as mean ± SD (n = 5 per group) and are representative of three independent experiments performed in triplicate. (K) Quantification of galectin-3 and arginase I positive macrophages in C57BL/6 mouse lungs 21 days after saline injection with or without subsequent hUC-MSC infusion. Results are mean ± SD of positive immunostained cell count per sample (n = 8 per group) and are representative of three independent experiments.</p
hUC-MSC down-regulate bleomycin-induced lung fibrosis.
No full textCollagen content in mouse lungs 8 days (A-D), 14 days (E-H) and 21 days (I-L) after endotracheal injection of sterile saline (saline) or bleomycin (bleomycin), the latter also followed by intravenous infusion of hUC-MSC (bleomycin+hUC-MSC) or sterile saline (bleomycin+saline). Lung sections obtained from C57BL/6 mice (n = 8 per group) were stained with Picrosirius Red. Controls (A,E,I) demonstrated normal lung architecture. 8 days post bleomycin injury, initial thickening of the alveoli and septa was observed (B). Collagen deposition progressively increased from day 8 to 21, with progressive distortion of lung architecture and formation of fibrotic foci (F,J). At each time point, bleomycin-induced alterations were significantly attenuated by hUC-MSC treatment (D,H,L), but not by saline (C,G,K). Representative microscopic images (10× magnification) of three independent experiments are shown. (M) The Ashcroft fibrosis score of lung sections obtained from C57BL/6 mice (n = 8 per group) that received the aforementioned treatments was calculated. Results are mean ± SD (n = 8 per group) and are representative of three independent experiments. * = P N) Hydroxyproline content in C57BL/6 mouse lungs that received the aforementioned treatments. Results are mean ± SD (n = 8 per group) expressed as a percent of the value obtained from endotracheal saline treated mice and are representative of three independent experiments. * = P < 0.05, ** = P < 0.01 compared to bleomycin treated mice.</p
hUC-MSC down-regulate bleomycin-induced lung inflammation.
No full textHistology of mouse lungs 8 days (A-D), 14 days (E-H) and 21 days (I-L) after endotracheal injection of sterile saline (saline) or bleomycin (bleomycin), the latter also followed by intravenous infusion of hUC-MSC (bleomycin+hUC-MSC) or sterile saline (bleomycin+saline). Lung sections obtained from C57BL/6 mice (n = 8 per group) were stained with H&E. Controls (A,E,I) demonstrated normal lung architecture. 8 days post bleomycin injury, peribronchial and perivascular inflammatory infiltrates were observed (B). Alveolar and interstitial infiltrates progressively increased from day 8 to 21, with progressive distortion of lung architecture and formation of fibrotic foci (F,J). At each time point, bleomycin-induced alterations were significantly attenuated by hUC-MSC treatment (D,H,L), but not by saline (C,G,K). Representative microscopic images (10× magnification) of three independent experiments are shown. (M) The histopathological inflammatory score of lung sections obtained from C57BL/6 mice (n = 8 per group) that received the aforementioned treatments was calculated. Results are mean ± SD (n = 8 per group) and are representative of three independent experiments. * = P < 0.05 compared to bleomycin treated mice.</p
The role of hSCs in promoting neural differentiation of hUC-MSCs in spinal cord injury
No full textQiuli Wu,1,* You Chen,1,* Guangzhi Ning,1 Shiqing Feng,1 Junling Han,2 Qiang Wu,1 Yulin LI,1 Hong Wu,1 Hongyu Shi1 1Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, People's Republic of China; 2Tianjin Union Stem Cell and Gene Engineering Co., Ltd, Tianjin, People's Republic of China * These authors contributed equally to this paper Abstract: Cell therapy is a promising approach to treating spinal cord injury (SCI). Previous studies demonstrated that co-transplantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) and human Schwann cells (hSCs) was an effective strategy by which to promote the regeneration of corticospinal fibers and locomotor recovery after SCI in rats. However, the neural differentiation potential of hUC-MSCs was not fully understood. In the present study, we examined the influence of hSCs on the survival and differentiation of hUC-MSCs in SCI rats. Four groups of rats were implanted with Dulbecco's Modified Eagle's Medium (DMEM), hSCs, hUC-MSCs, or a combination of hSCs and hUC-MSCs, respectively. Our results demonstrated that MAB1281 immunopositive cells appeared in the injured site of the transplanted cell groups, while myelin basic protein and high-molecular-weight neurofilament immunopositive cells were detected only in the co-transplantation group under the positive background of MAB1281. Furthermore, polymerase chain reaction (PCR) and Western blot showed significantly higher expression of myelin basic protein and high-molecular-weight neurofilament and lower expression of glial fibrillary acidic protein in the co-transplantation group (P < 0.05), which correlated strongly with immunofluorescence findings. These results suggest that hSCs could induce hUC-MSC differentiation into neurons and oligodendrocytes and inhibit the formation of glial scarring after SCI. The neural differentiation of hUC-MSCs is likely induced by soluble factors provided by hSCs. Keywords: spinal cord injury, Schwann cell, human umbilical cord mesenchymal stem cell, cell transplantation, neural differentiatio
Characterization of hUC-MSCs.
No full text(A) Growing cells from fragments (left) and MMC-treated hUC-MSCs feeder (right) show property of fibroblast-like cells with a spindle-shaped morphology. Scale bar: 200 μm. (B) The doubling time of different hUC-MSC lines. Each column represented the mean ±SD. (C) Flow cytometry results of rapidly dividing hUC-MSCs show they are positive for MSCs-specific markers (CD73, CD90 and CD105) but negative for CD34, CD45 and HLA-DR. (D) Alizarin Red S staining of osteogenic cells differentiated from hUC-MSCs (left). Scale bar: 100 μm. Oil Red O staining of adipogenic cells differentiated from hUC-MSCs (right). Scale bar: 20 μm.</p
Characterization and differentiation of hUC-MSCs.
No full text<p>A–G, Flow-cytometric analysis of cell surface antigens of hUC-MSC; H, Alizarin red S staining of osteogenic differentiated hUC-MSCs (400×); I, Oil red O staining of adipogenic differentiated hUC-MSCs (400×).</p
Análise proposta de reestruturação do Serviço de Sangue e Medicina Transfusional dos HUC, EPE
No full textHepatic differentiated hUC-MSCs can express hepatocyte–specific genes. A:
No full text<p>Hepatocyte-specific gene expression analyzed by qRT-PCR in hUC-MSCs and hepatic differentiated hUC-MSCs for 6 days, 14 days and 26 days. Hepatocyte-specific gene expression was normalized to GAPDH expression, and the results are expressed relative to a value of 1 in the control hUC-MSCs. <b>B:</b> immunofluorescence of hepatocyte-specific gene expression in MSCs and hepatic differentiated MSCs. After induction for 14 days, hUC-MSCs can express AFP, ALB and CK18.</p
Ryhiner-Kartensammlung / 20 Carte de la Manche des costes de France : parties de celle d'Espagne jusqu'au Cap Orgetal : verifiée sur plusieurs mémoires
No full textpar Mr. D. Ch. officier de marinesOsten obe
Hepatic differentiated hUC-MSCs possess hepatocyte-specific functions. A:
No full text<p>PAS staining of hUC-MSCs before and after hepatic differentiation using HK2 as a positive control. <b>B:</b> Analysis of the LDL uptake ability of hepatic differentiated hUC-MSCs. <b>C:</b> Analysis of the BUN synthetic ability of hepatic differentiated hUC-MSCs.</p
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