5,278 research outputs found

    The auto-generated fragment of the Usp1 deubiquitylase is a physiological substrate of the N-end rule pathway

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    Deamidation of N-terminal Gln by the Ntaq1 NtQ-amidase is a part of the Arg/N-end rule pathway, a ubiquitin-dependent proteolytic system. Here we identify Gln-Usp1Ct, the C-terminal fragment of the autocleaved Usp1 deubiquitylase, as the first physiological Arg/N-end rule substrate that is targeted for degradation through deamidation of N-terminal Gln. Usp1 regulates genomic stability, in part through the deubiquitylation of monoubiquitylated PCNA, a DNA polymerase processivity factor. The autocleaved Usp1 remains a deubiquitylase because its fragments remain associated with Uaf1, an enhancer of Usp1 activity, until the Gln-Usp1Ct fragment is selectively destroyed by the Arg/N-end rule pathway. We also show that metabolic stabilization of Gln-Usp1Ct results in a decreased monoubiquitylation of PCNA and in a hypersensitivity of cells to ultraviolet irradiation. Thus, in addition to its other functions in DNA repair and chromosome segregation, the Arg/N-end rule pathway regulates genomic stability through the degradation-mediated control of the autocleaved Usp1 deubiquitylase

    Isomorphisms in co-TT graphs

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    2019 Spring.Includes bibliographical references.A threshold tolerance graph is a graph where each vertex v is assigned a weight wv and a tolerance tv, and there is an edge between two vertices vx and vy if and only if wx + wy ≥ min(tx,ty). A co-TT graph is the complement of a threshold tolerance graph. Recognition of these graphs can be done in O(n2) time; however no polynomial-time algorithm to identify isomorphisms between pairs of TT or co-TT graphs was previously known. We give an algorithm to identify these isomorphisms, which takes O(n2) time

    Supplemental Material7 - Supplemental material for Half a century of Lighting Research & Technology: A bibliometric review

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    Supplemental material, Supplemental Material7 for Half a century of Lighting Research & Technology: A bibliometric review by QW Chen, TT Ru, DG Zhai, XH Huang, Y Li, L Qian, YY Wang and GF Zhou in Lighting Research & Technology</p

    Supplemental Material2 - Supplemental material for Half a century of Lighting Research & Technology: A bibliometric review

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    Supplemental material, Supplemental Material2 for Half a century of Lighting Research & Technology: A bibliometric review by QW Chen, TT Ru, DG Zhai, XH Huang, Y Li, L Qian, YY Wang and GF Zhou in Lighting Research & Technology</p

    Supplemental Material1 - Supplemental material for Half a century of Lighting Research & Technology: A bibliometric review

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    Supplemental material, Supplemental Material1 for Half a century of Lighting Research & Technology: A bibliometric review by QW Chen, TT Ru, DG Zhai, XH Huang, Y Li, L Qian, YY Wang and GF Zhou in Lighting Research & Technology</p

    Supplemental Material4 - Supplemental material for Half a century of Lighting Research & Technology: A bibliometric review

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    Supplemental material, Supplemental Material4 for Half a century of Lighting Research & Technology: A bibliometric review by QW Chen, TT Ru, DG Zhai, XH Huang, Y Li, L Qian, YY Wang and GF Zhou in Lighting Research & Technology</p

    Supplemental Material3 - Supplemental material for Half a century of Lighting Research & Technology: A bibliometric review

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    Supplemental material, Supplemental Material3 for Half a century of Lighting Research & Technology: A bibliometric review by QW Chen, TT Ru, DG Zhai, XH Huang, Y Li, L Qian, YY Wang and GF Zhou in Lighting Research & Technology</p
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