28,915 research outputs found
Detection and possible physiological significance of a-CEHC, an urinary metabolite of vitamin E
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Modulation of Prostaglandin E2 Production of a Macrophage Cell Line by Extracts of Foods Traditionally Regarded as “Heating” or “Cooling”
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Reduced Fat Mass in Rats Fed a High Oleic Acid–Rich Safflower Oil Diet Is Associated with Changes in Expression of Hepatic PPARα and Adipose SREBP-1c–Regulated Genes
No full textRats Fed a High Butter Diet are More Prone to Obesity and Glucose Intolerance Than Those Fed a High Safflower Oil Diet
No full textChanges in liver PPARα mRNA expression in response to two levels of high-safflower-oil diets correlate with changes in adiposity and serum leptin in rats and mice
No full textBitter gourd (Momordica charantia) extract activates peroxisome proliferator-activated receptors and upregulates the expression of the acyl CoA oxidase gene in H4IIEC3 hepatoma cells
No full text[[abstract]]Peroxisome proliferator-activated receptor (PPAR) is a ligand-dependent transcription factor that regulates the expression of genes involved in lipid metabolism and transport. Ligands/activators of PPAR, like fibrate-type drugs, may have hypolipidemic effects. To identify food that contains activators of PPAR, a transactivation assay employing a clone of CHO-K1 cells stably transfected with a (UAS)4-tk-alkaline phosphatase reporter and a chimeric receptor of Gal4-rPPAR LBD was used to screen ethyl acetate (EA) extracts of a large variety of food materials. It was found that the EA extract of bitter gourd(Momordica charantia), a common oriental vegetable, activated PPAR to an extent that was equivalent to or even higher than 10 ?M Wy-14643, a known ligand of PPAR. This extract also activated PPAR to a significant extent which was comparable to 0.5 ?M BRL-49653. The activity toward PPAR was mainly in the soluble fraction of the organic solvent. The EA extract prepared from the whole fruit showed significantly higher activity than that from seeds or flesh alone. The bitter gourd EA extract was then incorporated into the medium for treatment of a peroxisome proliferator-responsive murine hepatoma cell line, H4IIEC3, for 72 h. Treated cells showed significantly higher activity of acyl CoA oxidase and higher expressions of mRNA of this enzyme and fatty acid-binding protein, indicating that the bitter gourd EA extract was able to act on a natural PPAR signaling pathway in this cell line. It is thus worth further investigating the PPAR-associated health benefits of bitter gourd
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