16,743 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
Full text linkThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
Establishment and Characterization of A Human Lung Adenocarcinoma Cell Line, Ch-Huang-1
No full textBackground and objective Lung cancer is a major worldwide health problem. The aim of this study is to establish a novel Chinese human lung adenocarcinoma cell line and examine its biological characteristics. Methods Lung adenocarcinoma specimens were freshly resected during surgery. The tissues were incubated in vitro and the cell line was named Ch-Huang-1. The biological characteristics of the cells were investigated by light microscopy, chromosome analysis, and transplantation experiment. Results Light microscopy revealed that cells from the primary tumor, Ch-Huang-1 cell line, and transplanted tumor possessed the characteristics of a malignant glandular epithelial tumor. The cell growth curve, doubling time, and mitotic index were also observed in vitro. Nuclear chromosome analysis revealed that the tumor was a subtriploid with a mode of 35-44 per cell. Tumor nodes were observed under the skin of nude mice by heterogenic transplantation. Conclusion The characteristics of the established cell line suggest that it is a newly established human adenocarcinoma cell line
Dietary oxidized frying oil compromised vitamin A value of b-carotene in rats.(in Chinese)
No full textPioglitazone with sulfonylurea: glycemic and lipid effects in Taiwanese type 2 diabetic patients
No full textGanoderma products exert immunomodulatory activities in an ovalbumin immunized murine model
No full textFatigue resistance analysis of tibial baseplate in total knee prosthesis- an in vitro biomechanical study
No full textTorsion - vibration couplings in the CH3OO· radical
Full text linkA partially rotationally resolved infrared spectrum of CHOO in the CH stretch region has been reported\footnote{K.-H.~Hsu, Y.-H.~Huang, Y.-P.~Lee, M.~Huang, T.~A.~Miller and A.~B.~McCoy \textit{J.~Phys.~Chem.~A}, in press, DOI: 10.1021/acs.jpca.5b12334}. The rotational contour of the CH stretch band in the experimental spectrum can be simulated with an asymmetric rotor model. The simulation shows good agreement with the experimental spectrum except that the broadening of the Q-branch in the experimental spectrum remains unexplained. This broadening is likely due to the sequence band transitions from the torsionally excited levels populated at room temperature to combination levels involving the CH stretch and the same number of torsional quanta. A four dimension model involving three CH stretches and the CH torsion is applied to the CHOO radical to obtain the frequencies and intensities of the vibrational transitions in the CH stretch region. Based on these calculations, the torsional sequence bands are calculated to be slightly shifted from the origin band, because of the couplings between the CH stretches and CH torsion, thereby causing the apparent broadening observed for the fundamental. Due to the accidental degeneracy of two different CH stretch and CH torsion combination levels which differ by one quantum in the torsional excitation, the frequencies of the torsional sequence bands will be very sensitive to details of the potential, which makes the shifts difficult to precisely predict with electronic structure calculations. Complementary analyses are now underway for the other two CH stretch vibrational bands, and .Made available in DSpace on 2017-01-26T21:38:16Z (GMT). No. of bitstreams: 3
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Previous issue date: 2016-06-2
Purification and cloning of carp nephrosin, a secreted zinc endopeptidase of the astacin family.
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