111,012 research outputs found
Construction of hu-C4BP, hu-FH and C4BPxFH tg BALB/c mice.
No full text<p>(<b>A</b>) The depicted constructs were used to generate transgenic animals that expressed human C4BP and/or FH in addition to their endogenous counterpart C4BP and FH molecules. (<b>B</b>) PCR analysis confirmed the presence of the either hu-C4BP α-chains (upper panel), human Factor H (FH) (lower panel) or both (both panels) in the tg animals, but not in BALB/c wt mice. <b>(C)</b> SDS-PAGE and western blot analysis confirmed that hu-C4BP (<b>C</b>, upper panel non-reduced gel) and hu-FH (<b>C</b>, lower panel, reduced gel) were detectable in the appropriately designated tg, but not in BALB/c mouse serum. (<b>D</b>) Serum levels of hu-C4BP and hu-FH were determined using a sandwich-ELISA. (<b>E)</b> BALB/c (blue) and hu-C4BPxFH tg (red) serum (20%) deposit similar amounts of C3 on zymosan particles by flow cytometry. EDTA-treated BALB/c serum (negative control) did not deposit any C3 on zymosan (negative control; n = 3 sera from individual animals). Statistical analysis: Kruskal-Wallis analysis with Dunn’s post-test (<b>D</b>) and 1-way ANOVA with Bonferroni’s post-test (<b>E</b>).</p
Second-harmonic generation on simple metal surfaces excited by p-polarized light: The volume current
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Correlation between neutralising antibody titres against vaccine (Wuhan-hu-1) and VOCs.
No full textAntibody responses measured by pseudotype-based neutralisation assay against Wuhan-hu-1 were compared with those against B.1.617.1, B.1.617.2 and B.1.351. Responses were compared for A) BNT162b2 1 dose, B) BNT162b2 2 doses, C) ChAdOx1 1 dose and D) ChAdOx1 2 doses. Correlations between groups were evaluated and non-parametric Spearman correlation coefficients calculated using GraphPad Prism version 8. (DOCX)</p
Relationship between ferroelectricity and Dzyaloshinskii-Moriya interaction in multiferroics and the effect of bond-bending
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