1,562 research outputs found
Cloning and characterization of the sigma factor gene from Leptospira interrogans serovar copenhageni.
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Thickness-dependent smectic-A - smectic-C* transition in chiral smectic free-standing liquid-crystal films
No full textUnusual thickness-dependent thermal behavior due to molecular tilt coupling strength in free-standing 7O.7 thin films
No full textSevere obstetric complications and birth characteristics in preterm or term delivery were accurately recalled by mothers
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Successful resuscitation of acute massire pulmonary embolism with extracorporeal membrane oxygenation and open embolectomy.
No full textPreferential angiogenic induction ability of WJ-MSCs.
No full text<p>(<b>A</b>–<b>B</b>) HMEC1 endothelial cells <i>in vitro</i> motility. Migrated HMEC1 that were attracted by BM-MSCs and WJ-MSCs were counted. MSC culture medium was used as negative control (medium only). Representative images of migrated HMEC1 cells are shown (B). (<b>C</b>–<b>D</b>) HMEC1 cells <i>in vitro</i> tube formation using Matrigel at 4 hours incubation in conditioned medium from BM-MSCs or conditioned medium from WJ-MSCs. Representative images of the HMEC1 tube formation are shown (D). (*<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001).</p
Differential gene expression between WJ-MSCs and BM-MSCs.
No full text<p>(<b>A</b>) Flow cytometry analysis of WJ-MSCs and BM-MSCs. (<b>B</b>–<b>C</b>) A total of 1096 probe sets (q ≦ 10<sup>-3</sup>) differentiating WJ-MSCs and BM-MSCs were filtered out and 762 WJ-MSC probe sets (B) and 334 BM-MSC probe sets (C) were subjected to DAVID (<a href="http://david.abcc.ncifcrf.gov/" target="_blank"><u>http://david.abcc.ncifcrf.gov/</u></a>) analysis. These categories were selected using the Biological Process organizing principle via the Gene Ontology project (<a href="http://www.geneontology.org/" target="_blank"><u>http://www.geneontology.org/</u></a>). The number of genes, as well as <i>p</i> values, for categories that are significantly (<i>p</i><0.05) over-represented are listed. The terms indicated by arrows are discussed in the text. Genes involved in nervous system development and blood vessel development are listed, and RT-qPCR verified genes are underlined. (<b>D</b>) RT-qPCR validation of the relative expression levels of the neurogenic-related and angiogenic-related genes in the two MSC subtypes. Mean expression levels of the target genes were compared to that of the GAPDH control. Each bar represents a different individual. Results were expressed as the mean ± standard deviation (SD). (<b>E</b>) A heatmap showing BM-MSC and WJ-MSC-specific secreted factors. (<b>F</b>–<b>G</b>) Validation of array data by RT-qPCR. <i>ANGPT1</i> and <i>PGF</i>, which are up-regulated in BM-MSCs (<b>F</b>), as well as various WJ-MSC abundant genes (<b>G</b>), were examined. (<b>H</b>) The secretion levels of CXCL5 and PGF in the conditioned medium of BM-MSC and WJ-MSC were quantified using enzyme-linked immunosorbent assays. Each bar represents the protein concentration of independent donors.</p
Preferential neuroprotection effects of WJ-MSCs.
No full text<p>(<b>A</b>) Schematic representation of the transmembranous stem cell co-culture system using an oxygen-glucose deprivation (OGD) model. (<b>B</b>) Immunofluorescence staining of the rat primary cortical cells subjected to OGD alone (left), to co-culture with BM-MSCs (middle), or to co-culture with WJ-MSCs (right) at 72 hours post-OGD. Neuronal marker MAP2 is shown in red, the astroglial marker GFAP in green, and DAPI nuclear staining in blue. Scale bar: 20 µm. (<b>C</b>) Quantification of cell death and apoptosis rate using PI and TUNEL staining, respectively, at 72 hours post-OGD. *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001 (<b>D</b>) Quantification of total neurite length (left) and neurite branch point numbers (right). (<b>E</b>) Percentage of neuron number (left) and astrocyte number (right) after 72 hours co-cultured with BM-MSCs or WJ-MSCs post-OGD.</p
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