7,934 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
Full text linkThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
Scanning tunneling microscopy observations of butanethiol self-assembled monolayers on Ag underpotential deposition modified Au(111)
No full textSummer spatial distribution of copepods and fish larvae in relation to hydrography in the northern Taiwan strait.
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Fatigue resistance analysis of tibial baseplate in total knee prosthesis- an in vitro biomechanical study
No full textIntegration of separation capillary with a Au film electrode and an etched decoupler in amperometric capillary electrophoresis
No full textMorphological comparisons of Taiwan native wild tea plant (Camellia sinensis (L.) O. Kuntze forma formosensis Kitamura) and two closely related taxa using numerical methods.
No full textComposition and abundance of copepods and ichthyoplankton in Taiwan Strait (western North Pacific) are influenced by seasonal monsoons.
No full textCopepod diversity and composition as indicators of intrusion of the Kuroshio Branch Current into the northern Taiwan Strait in spring 2000.
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