8 research outputs found
Leopold von Kalckreuth, poetischer Realist [aus Anlaß der Ausstellung "Leopold von Kalckreuth - Poetischer Realist", 30. September 2005 bis 1. Januar 2006 in der Hamburger Kunsthalle]
No full textAdolph Menzel und Lois Renner - das Künstleratelier [aus Anlass der Ausstellung "Adolph Menzel und Lois Renner - Das Künstleratelier" vom 9. August bis 2. November 2008 in der Hamburger Kunsthalle]
No full textCharacterising plasmacytoid and myeloid AXL<sup>+</sup> SIGLEC-6<sup>+</sup> dendritic cell functions and their interactions with HIV
No full textCopyright: \ua9 2024 Warner van Dijk et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. AXL+ Siglec-6+ dendritic cells (ASDC) are novel myeloid DCs which can be subdivided into CD11c+ and CD123+ expressing subsets. We showed for the first time that these two ASDC subsets are present in inflamed human anogenital tissues where HIV transmission occurs. Their presence in inflamed tissues was supported by single cell RNA analysis of public databases of such tissues including psoriasis diseased skin and colorectal cancer. Almost all previous studies have examined ASDCs as a combined population. Our data revealed that the two ASDC subsets differ markedly in their functions when compared with each other and to pDCs. Relative to their cell functions, both subsets of blood ASDCs but not pDCs expressed co-stimulatory and maturation markers which were more prevalent on CD11c+ ASDCs, thus inducing more T cell proliferation and activation than their CD123+ counterparts. There was also a significant polarisation of na\uefve T cells by both ASDC subsets toward Th2, Th9, Th22, Th17 and Treg but less toward a Th1 phenotype. Furthermore, we investigated the expression of chemokine receptors that facilitate ASDCs and pDCs migration from blood to inflamed tissues, their HIV binding receptors, and their interactions with HIV and CD4 T cells. For HIV infection, within 2 hours of HIV exposure, CD11c+ ASDCs showed a trend in more viral transfer to T cells than CD123+ ASDCs and pDCs for first phase transfer. However, for second phase transfer, CD123+ ASDCs showed a trend in transferring more HIV than CD11c+ ASDCs and there was no viral transfer from pDCs. As anogenital inflammation is a prerequisite for HIV transmission, strategies to inhibit ASDC recruitment into inflamed tissues and their ability to transmit HIV to CD4 T cells should be considered
Physical symptom profile for adult cancer inpatients at a Lebanese cancer unit
No full textBackground: Hospital-based palliative care programs in Lebanon are nonexistent in a structured form. One of obstacles is the lack of knowledge about symptom prevalence and burden of cancer patients in Lebanon. Methods: This is a cross-sectional observational study where 100 adult cancer patients admitted to the American University of Beirut Medical Center inpatient unit completed a survey to assess 20 physical symptoms according the National Cancer Institute's Common Terminology Criteria for Adverse Events 4.0 (NCI-CTCAE 4.0) guidelines. Results: Hematologic, gastrointestinal, breast, and lung cancers were the most common. Mean age was 51.5 years; 51percent were female. 74percent of patients with solid tumors had metastatic disease. Treatment approaches were palliative chemotherapy, followed by curative chemotherapy and best supportive care. The most common symptoms were fatigue, appetite loss, nausea, and pain; most distressing were nausea, pain, and fatigue. Nausea and vomiting were more prevalent among females than males. Females reported more severe vomiting than males, but males had more intense pain. Overall symptom burden difference was statistically significant across age groups, with the 51-60 year group having the most symptoms, but not among different genders. Difference was significant among different treatment intents, with the best supportive care group having most symptoms. Conclusion: Fatigue should be better addressed as a legitimate symptom. Subgroup differences must be considered when managing gastrointestinal symptoms. Pain should be more effectively managed, and vulnerable subgroups such as the 51-60 year age group and those on best supportive care should receive special consideration. © 2012 European Federation of Internal Medicine.Curt G A, 2000, Oncologist, V5, P353, DOI 10.1634-theoncologist.5-5-353; Curtis E B, 1991, J Palliat Care, V7, P25; Daher M, 2002, J PAIN SYMPTOM MANAG, V24, P200, DOI 10.1016-S0885-3924(02)00463-3; Daher Michel, 2008, J Med Liban, V56, P70; DONNELLY S, 1995, SEMIN ONCOL, V22, P67; Dunlop G, 1989, PALLIATIVE MED, V4, P37, DOI DOI 10.1177-026921639000400108; Dy SM, 2010, CANCER J, V16, P500, DOI 10.1097-PPO.0b013e3181f45853; Education for Physicians on End-of-Life Care EPEC, 1999, ED PHYS END OF LIF C; JENNS K, 1994, CANCER NURS, V17, P488; Komurcu S, 2000, SEMIN ONCOL, V27, P24; Mock V, 2000, ONCOLOGY-NY, V14, P151; Morrison RS, 2005, J PALLIAT MED, V8, P1127, DOI 10.1089-jpm.2005.8.1127; Naccache Nicole, 2008, J Med Liban, V56, P77; Naifeh Khoury M, 2008, J MED LIBAN, V56, P83; Pan C X, 2001, J Palliat Med, V4, P315, DOI 10.1089-109662101753123922; Radbruch L, 2008, PALLIATIVE MED, V22, P13, DOI 10.1177-0269216307085183; Teunissen SCCM, 2007, J PAIN SYMPTOM MANAG, V34, P94, DOI 10.1016-j.jpaisymman.2006.10.015; Vogelzang NJ, 1997, SEMIN HEMATOL, V34, P4; Wagner LI, 2004, BRIT J CANCER, V91, P822, DOI 10.1038-sj.bjc.6602012; World Health Organization, 2006, WHO DEF PALL CAR; World Health Organization, 2002, NAT CANC CONTR PROGR12
Greening and development in wheat seedlings
No full textThe greening of etiolated first leaf blades of wheat (Triticium aestivum Mercia) seedlings (referred to in the text as leaf tissue) was studied in relation to tissue age and water stress. Use was made of whole seedlings, excised leaf blades and leaf blade segments. Responses to photoperiodic illumination were measured as changes in the levels of chlorophyll, total soluble protein and nucleic acids (both total and specific fractions). The pattern of greening in the whole seedlings, excised leaf blades and leaf blade segments was essentially the same in 6 and 10 day-old dark-grown tissues, where chlorophyll accumulation followed the age sequence along the leaf. Least chlorophyll accumulated in the tip of leaves of both ages but the older leaves contained less overall pigment than the younger leaves. Patterns of total soluble protein and total nucleic acid accumulation did not reflect the pattern shown by the chlorophyll. Protein accumulated most in the tip region, with nucleic acids being highest in the middle region. Water stress treatment reduced chlorophyll accumulation in leaf blade tissue, particularly in the intact seedlings. Protein levels, however, were more variable and appeared to reflect the ability of the younger tissue to accumulate this compound as a stress metabolite. Total nucleic acid levels were also elevated under water stress. Again, these effects were most marked on the intact seedlings, implying that an effect on the roots was also involved. The data from polyacrylamide gel electrophoresis of RNA fractions showed that the level of chloroplast RNA components was maintained up to 17 days for tissue incubated in the dark as well as in the light. Severe water stress treatments applied to the roots of whole seedlings resulted in the loss of ribosomal fraction in the leaves. However, this effect was not seen with mild water stress. Kinetin treatments during water stress did not appear to alter the pattern of cell component accumulation although in unstressed material, treatment with this compound enhanced chlorophyll accumulation slightly, especially in the young tissue. This indicates that, at the concentration used, the growth regulator was not able to alleviate the stress condition. The leaf blades of intact seedlings responded in a specific way which was much more pronounced than for excised leaf blades or leaf blade segments. It was concluded that some signalling was involved between the root and shoot tissues during water stress treatments
