8,322 research outputs found
Kinetic Study of Organic Acid Formations and Growth of Anaerobiospirillum succiniciproducens During Continuous Cultures
No full textSuccinic acid-producing Anaerobiospirillum succiniciproducens was anaerobically grown in glucose-fed continuous cultures using glucose as a carbon source, and the metabolic flexibility of A. succiniciproducens in response to varying glucose concentrations and dilution rates was examined. Both succinic acid (SA) and acetic acid (AA) formation was growth-associated, and their growth-rate-related coefficients (K(SA/X), K(AA/X)) and nongrowth-rate-related coefficients (K'(SA/X), K'(AA/X)) were slightly influenced by glucose concentrations. A high glucose concentration (38 g/l) and high growth rate (0.63 h(-1)) did not induce by-product formation.This work was supported by a grant from Research
Promotion Program funded by Gyeonggi Province and by
Priority Research Centers Program through the National
Research Foundation of Korea (NRF) funded by the Ministry
of Education, Science and Technology (20090093826) (PC
Lee) and by the Genome based Integrated Bioprocess
Development Project from the Ministry of Education,
Science and Technology (SY Lee and HN Chang)
Cell recycled culture of succinic acid-producing Anaerobiospirillum succiniciproducens using an internal membrane filtration system
No full textCell recycled culture of succinic acid-producing Anaerobiospirillum succiniciproducens was anaerobically carried out using an internal membrane filter module in order to examine the physiological response of A. succiniciproducens to a high-cell-density environment. The optimal growth of A. succiniciproducens and its enhanced succinic acid productivity were observed under CO2-rich conditions, established by adding NaHCO3 and Na2CO3, in the cell recycled system. A. succiniciproducens grew up to 6.50 g-DCW/l, the highest cell concentration obtained so far, in cell recycled cultures. The cells did not change their morphology, which is known to be easily changed in unfavorable or stress environments. The maximum productivity of succinic acid was about 3.3 g/l/h, which is 3-3 times higher than those obtained in batch cultures. These results can serve as a guide for designing highly efficient cell recycled systems for succinic acid at a commercial level.This work was supported by the Genome-based Integrated
Bioprocess Development Project of the Ministry of Science
and Technology through the Korea Science and Engineering
Foundation. Further support by the LG Chem Chair
Professorship (SYL) is appreciated
On the amperometric detection and electrocatalytic analysis of ascorbic acid and dopamine using a poly(acriflavine)-modified electrode
No full textSuccinic Acid Production by Anaerobiospirillum succiniciproducens ATCC 29305 Growing on Galactose, Galactose/Glucose, and Galactose/Lactose
No full textSuccinic acid-producing Anaerobiospirillum succiniciproducens was anaerobically grown on galactose, galactose/glucose, or galactose/lactose in order to study its galactose fermentation. Unlike a previous report, A. succiniciproducens was found to efficiently metabolize galactose as the sole carbon source at a rate of 2.4 g/g-DCW/h and produced succinic acid with as high a yield of 87% as with using glucose. When glucose and galactose were present, A. succiniciproducens metabolized both sugars simultaneously. Furthermore, when lactose and galactose coexisted, lactose did not inhibit the galactose fermentation of A. succiniciproducens. Therefore, co-utilization of galactose and other sugars can improve the productivity and economy of bio-based succinic acid processes.This work was supported by grants from Ajou University
to PCL and by the Genome-based Integrated Bioprocess
Development Project of the Ministry of Science and
Technology through the Korea Science and Engineering
Foundation. Further support by the LG Chem Chair
Professorship (SYL) is appreciated
Determinants of return to work among permanently disabled workers resulting from occupational injury
No full textFabricating an Amperometric Cholesterol Biosensor by a Covalent Linkage between Poly(3-thiopheneacetic acid) and Cholesterol Oxidase
No full textIn this study, use of the covalent enzyme immobilization method was proposed to attach cholesterol oxidase (ChO) on a conducting polymer, poly(3-thiopheneacetic acid), [poly(3-TPAA)]. Three red-orange poly(3-TPAA) films, named electrodes A, B and C, were electropolymerized on a platinum electrode by applying a constant current of 1.5 mA, for 5, 20 and 100 s, respectively. Further, 1-ethyl-3-(3-dimethylamiopropyl)carbodiimide hydrochloride (EDC‧HCl) and N-hydroxysuccinimide (NHS) were used to activate the free carboxylic groups of the conducting polymer. Afterwards, the amino groups of the cholesterol oxidase were linked on the activated groups to form peptide bonds. The best sensitivity obtained for electrode B is 4.49 mA M-1 cm-2,with a linear concentration ranging from 0 to 8 mM, which is suitable for the analysis of cholesterol in humans. The response time (t95) is between 70 and 90 s and the limit of detection is 0.42 mM, based on the signal to noise ratio equal to 3. The interference of species such as ascorbic acid and uric acid increased to 5.2 and 10.3% of the original current response, respectively, based on the current response of cholesterol (100%). With respect to the long-term stability, the sensing response retains 88% of the original current after 13 days
A PC parallel port button box provides millisecond response time accuracy under Linux
No full textFor psychologists, it is sometimes necessary to measure people's reaction times to the nearest millisecond. This article describes how to use the PC parallel port to receive signals from a button box to achieve millisecond response time accuracy. The workings of the parallel port, the corresponding port addresses, and a simple Linux program for controlling the port are described. A test of the speed and reliability of button box signal detection is reported. If the reader is moderately familiar with Linux, this article should provide sufficient instruction for him or her to build and test his or her own parallel port button box. This article also describes how the parallel port could be used to control an external apparatus
Effective applications of microcomputer-based management information and decision support systems for small and medium sized enterprises
No full textThis thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.Firstly, this thesis reviews the literature on the application of microcomputer-based Management Information Systems (MISs) and Decision Support Systems (DSS) to Small and Medium sized Enterprises (SMEs). It is found that the hardware platform today is already sufficient for SMEs. However, information regarding successful implementation of MISs for SMEs is scarce and largely fragmented.
DSS requires more focused and dedicated use of information to support managerial decision making. Unfortunately, the development of DSSs for SMEs is even more backward. Yet, there is an emerging need for SMEs today because business operations have become more sophisticated under intensified
competition.
With this scenario in mind, the author undertook intensive questionnaire and case surveys to find out the current development and trends for the effective applications of MISs and DSSs. In 1987, the author was awarded the Oshikawa Fellowship by Asian Productivity Organisation in Tokyo and started the present research. 446 completed questionnaire survey sheets from U.K. and Hang Kong have been received and analysed. 67 SMEs and related organisations in 6 developing/developed countries were also visited. This forms the knowledge for the development of expert systems (ES) for effective applications of MIS.
The approach for DSS is based on a carefully selected business game which has most of the common business decision parameters. Intensive experiment with over 100 subjects was conducted in running the game, with an average time contribution of about 20 hours/person. The findings are again consolidated and structured into an ES.
Longitudinal research was conducted in 5 representative SMEs. With the use of action learning and participation of the researcher, more in-depth firsthand information were obtained and analysed. These form part of the input to the ES as well.
Both ES have been validated and further improved. The experimenters find these as keys to develop MIS/DSS for SMEs. A marketing plan is suggested to launch these two products so that they can become more easily available. Finally, recommendations are made on the effective use of the ES and for further development
GluRδ2 induces spinogenesis in the PC proximal dendritic compartment of δ2/GFP-ho mice.
No full text<p>(A–D) Immunostaining of PC proximal dendrites in δ2/GFP-ho (A–C) and GFP-ho mice (D). In δ2/GFP-ho mice, many new spines, expressing the GluRδ2 subunit (red) (B and C), appears in the proximal dendrite relative to GFP-ho mice (D). (E) Histogram shows the mean spine density in the proximal dendritic domain. In the presence of GluRδ2, the number of spines significantly increases relative to control groups (GFP-wt; GFP-ho and δ2/GFP-ho CTR). *** p<0.001. Error bars indicate SE. Scale bars: A–E = 2 µm.</p
Emergence of nosocomial candidemia at a teaching hospital in Taiwan from 1981 to 2000: increased susceptibility of Candida species to fluconazole.
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