462,544 research outputs found
Effects of HO-1 overexpression on mitochondrial dysfunction and autophagy level in Lv-HO-1-H9c2 cells with H/R model.
No full text(A) Cell viability was measured using the CCK-8 assay. The data are presented as means ± SE (N = 5). **p<0.01 vs corresponding Normoxia group; #p<0.05, vs corresponding Lv-scramble group. (B) Real-time quantitative PCR (RT q-PCR) analyses of HO-1 mRNA expression in lv-HO-1 H9c2 cells subjected to H/R relative to GAPDH expression (n = 3 wells per group). **p<0.01 vs. the normoxia group. (C) H/R-induced HO-1, p62, and LC-3 protein expression analyzed using western blots of lv-HO-1 H9c2 cells. GAPDH was used as a loading control (n = 3 wells per group). ##p<0.01 vs corresponding Lv-scramble group; ***p<0.001 vs. the corresponding normoxia group. (D) Representative confocal microscopy images and quantitative analysis of autophagosomes from 15 fields (n = 3 hearts per group). Scale bar = 500 nm. *p<0.05 vs. the corresponding normoxia group; ##p<0.01, vs corresponding Lv-scramble group. H/R, Hypoxia/reoxygenation; HO-1, heme oxygenase-1. (E,F) Flow cytometry detection of changes in JC-1 fluorescence color reflects changes in the mitochondrial membrane potential and mitochondrial ROS levels. #p<0.05, vs corresponding Lv-scramble group; **p<0.01 vs corresponding Normoxia group, #p<0.05, vs corresponding Lv-scramble group. (G) The apoptosis rate of the four groups. Cell identification and detection of apoptosis. **p<0.01 vs corresponding Normoxia group; ##p<0.01, vs corresponding Lv-scramble group.</p
The Ho-Zhao problem
Get PDFGiven a poset P, the set Γ(P) of all Scott closed sets ordered by inclusion forms a complete lattice. A subcategory C of Posd (the category of posets and Scott-continuous maps) is said to be Γ-faithful if for any posets P and Q in C, Γ(P) ∼= Γ(Q) implies P ∼= Q. It is known that the category of all continuous dcpos and the category of bounded complete dcpos are Γ-faithful, while Posd is not. Ho & Zhao (2009) asked whether the category DCPO of dcpos is Γ-faithful. In this paper, we answer this question in the negative by exhibiting a counterexample. To achieve this, we introduce a new subcategory of dcpos which is Γ-faithful. This subcategory subsumes all currently known Γ-faithful subcategories. With this new concept in mind, we construct the desired counterexample which relies heavily on Johnstone’s famous dcpo which is not sober in its Scott topology
HO-1 and HIF-1α are expressed in the peri-infarct region of the ischemic mouse brain.
No full text(A) Representative image of the TTC stained regions (a, contralateral region; b, peri-infarct region; c, infarct region) in a mouse subjected to 2 h ischemia and 24 h reperfusion (I/R) (n = 3 per group). (B) DAB staining observed as brown color in the peri-infarct region (b) in wild-type (WT) and HO-1+/- mice. Scale bars = 20 μm. (C) Expression of target proteins was determined in brain tissues using western blot analysis, and their levels were quantified (n = 5 per group). **P D) WT and HO-1+/- mice were subjected to I/R, and the brain sections (a, contralateral region; b, peri-infarct region; c, infarct region) were stained with the indicated antibodies (n = 4 per group). Images are representative from three individual tissues.</p
HO-1 expression in macrophages.
No full textRepresentative sections (400x magnifications) of rat prostate MatLyLu TINT double stained for CD68+ (brow) and HO-1+ (red) cells (A), or double stained for CD163+ (brown) and HO-1+ (red) cells (B). Most cells stained both red and blown suggesting that most HO-1+ cells are macrophages, and in particular of the M2-type (CD163+).</p
The 1961 Kampong Bukit Ho Swee fire and the making of modern Singapore
Get PDFBy 1970, Singapore’s urban landscape was dominated by high-rise blocks of planned public housing built by the People’s Action Party government, signifying the establishment of a high modernist nation-state. A decade earlier, the margins of the City had been dominated by kampongs, home to semi-autonomous communities of low-income Chinese families which freely built, and rebuilt, unauthorised wooden houses. This change was not merely one of housing but belied a more fundamental realignment of state-society relations in the 1960s. Relocated in Housing and Development Board flats, urban kampong families were progressively integrated into the social fabric of the emergent nation-state. This study examines the pivotal role of an event, the great Kampong Bukit Ho Swee fire of 1961, in bringing about this transformation. The redevelopment of the fire site in the aftermath of the calamity brought to completion the British colonial regime’s ‘emergency’ programmes of resettling urban kampong dwellers in planned accommodation, in particular, of building emergency public housing on the sites of major fires in the 1950s. The PAP’s far greater political resolve, and the timing of and state of emergency occasioned by the scale of the 1961 disaster, enabled the government to rehouse the Bukit Ho Swee fire victims in emergency housing in record time. This in turn provided the HDB with a strategic platform for clearing other kampongs and for transforming their residents into model citizens of the nation-state. The 1961 fire’s symbolic usefulness extended into the 1980s and beyond, in sanctioning the PAP’s new housing redevelopment schemes. The official account of the inferno has also become politically useful for the government of today for disciplining a new generation of Singaporeans against taking the nation’s progress for granted. Against these exalted claims of the fire’s role in the Singapore Story, this study also examines the degree of actual change and continuity in the social and economic lives of the people of Bukit Ho Swee after the inferno. In some crucial ways, the residents continued to occupy a marginal place in society while pondering, too, over the unresolved question of the cause of the fire. These continuities of everyday life reflect the ambivalence with which the citizenry regarded the high modernist state in contemporary Singapore
HMOX1 gene promoter alleles and high HO-1 levels are associated with severe malaria in Gambian children.
Get PDFHeme oxygenase 1 (HO-1) is an essential enzyme induced by heme and multiple stimuli associated with critical illness. In humans, polymorphisms in the HMOX1 gene promoter may influence the magnitude of HO-1 expression. In many diseases including murine malaria, HO-1 induction produces protective anti-inflammatory effects, but observations from patients suggest these may be limited to a narrow range of HO-1 induction, prompting us to investigate the role of HO-1 in malaria infection. In 307 Gambian children with either severe or uncomplicated P. falciparum malaria, we characterized the associations of HMOX1 promoter polymorphisms, HMOX1 mRNA inducibility, HO-1 protein levels in leucocytes (flow cytometry), and plasma (ELISA) with disease severity. The (GT)(n) repeat polymorphism in the HMOX1 promoter was associated with HMOX1 mRNA expression in white blood cells in vitro, and with severe disease and death, while high HO-1 levels were associated with severe disease. Neutrophils were the main HO-1-expressing cells in peripheral blood, and HMOX1 mRNA expression was upregulated by heme-moieties of lysed erythrocytes. We provide mechanistic evidence that induction of HMOX1 expression in neutrophils potentiates the respiratory burst, and propose this may be part of the causal pathway explaining the association between short (GT)(n) repeats and increased disease severity in malaria and other critical illnesses. Our findings suggest a genetic predisposition to higher levels of HO-1 is associated with severe illness, and enhances the neutrophil burst leading to oxidative damage of endothelial cells. These add important information to the discussion about possible therapeutic manipulation of HO-1 in critically ill patients
Study of Ho-doped Bi2Te3 topological insulator thin films
Get PDFThis publication arises from research funded by the John Fell Oxford University Press (OUP) Research Fund and the Research Complex at Harwell is acknowledged for their hospitality. This work was supported by a DARPA MESO project (No. N66001-11-1-4105). S.E.H. was supported by the VPGE (Stanford University). L.C.M. and A.A.B. acknowledge partial financial support from EPSRC (UK) through a Doctoral Training Award. Diamond Light Source is acknowledged for beamtime on I10 (proposal SI10207).Breaking time-reversal symmetry through magnetic doping of topological insulators has been identified as a key strategy for unlocking exotic physical states. Here, we report the growth of Bi2Te3 thin films doped with the highest magnetic moment element Ho. Diffraction studies demonstrate high quality films for up to 21% Ho incorporation. Superconducting quantum interference device magnetometry reveals paramagnetism down to 2 K with an effective magnetic moment of ∼5 μB/Ho. Angle-resolved photoemission spectroscopy shows that the topological surface state remains intact with Ho doping, consistent with the material's paramagnetic state. The large saturation moment achieved makes these films useful for incorporation into heterostructures, whereby magnetic order can be introduced via interfacial coupling.Peer reviewe
Cooling rates of neutron stars and the young neutron star in the Cassiopeia A supernova remnant
No full textWe explore the thermal state of the neutron star in the Cassiopeia A supernova remnant using the recent result of Ho & Heinke that the thermal radiation of this star is well described by a carbon atmosphere model and the emission comes from the entire stellar surface. Starting from neutron star cooling theory, we formulate a robust method to extract neutrino cooling rates of thermally relaxed stars at the neutrino cooling stage from observations of thermal surface radiation. We show how to compare these rates with the rates of standard candles – stars with non-superfluid nucleon cores cooling slowly via the modified Urca process. We find that the internal temperature of standard candles is a well-defined function of the stellar compactness parameter x=rg/R, irrespective of the equation of state of neutron star matter (R and rg are circumferential and gravitational radii, respectively). We demonstrate that the data on the Cassiopeia A neutron star can be explained in terms of three parameters: f?, the neutrino cooling efficiency with respect to the standard candle; the compactness x; and the amount of light elements in the heat-blanketing envelope. For an ordinary (iron) heat-blanketing envelope or a low-mass (? 10?13 M?) carbon envelope, we find the efficiency f?? 1 (standard cooling) for x? 0.5 and f?? 0.02 (slower cooling) for a maximum compactness x? 0.7. A heat blanket containing the maximum mass (?10?8 M?) of light elements increases f? by a factor of 50. We also examine the (unlikely) possibility that the star is still thermally non-relaxe
A 2 h periodic variation in the low-mass X-ray binary Ser X-1
No full textSpectroscopy of the low-mass X-ray binary Ser X-1 using the Gran Telescopio Canarias have revealed a ?2 h periodic variability that is present in the three strongest emission lines. We tentatively interpret this variability as due to orbital motion, making it the first indication of the orbital period of Ser X-1. Together with the fact that the emission lines are remarkably narrow, but still resolved, we show that a main-sequence K dwarf together with a canonical 1.4 M? neutron star gives a good description of the system. In this scenario, the most likely place for the emission lines to arise is the accretion disc, instead of a localized region in the binary (such as the irradiated surface or the stream-impact point), and their narrowness is due instead to the low inclination (?10°) of Ser X-1
HO-1 induction promotes M2 macrophage polarisation.
No full textRAW 264.7 cells were transfected with siRNA HO-1 and siRNA NC (non-correlated). (A) RT-qPCR analysis of HO-1 mRNA in cells transfected 48 hours after transfection. Cells were treated with SP (10 μM) and/or LPS (100 ng/ml) for 24 hours and mRNA expression of selected genes was evaluated by RT-qPCR (B) IL-6 mRNA expression (C) TNF-α mRNA expression (D) Arg1 mRNA expression (E) IL-10 mRNA expression and (F) Rel A mRNA expression. The data are mean ±SD of two separate experiments, each of which was performed in triplicate. **p < 0.01 versus siRNA NC.</p
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