8,413 research outputs found
Suppression of the Pth(Ts) phenotype mediated by the overproduction of tRNA maintains moderate levels of the Pth(Ts) protein
No full text<p><b>Copyright information:</b></p><p>Taken from "Excess of charged tRNA maintains low levels of peptidyl-tRNA hydrolase in (Ts) mutants at a non-permissive temperature"</p><p>Nucleic Acids Research 2006;34(5):1564-1570.</p><p>Published online 15 Mar 2006</p><p>PMCID:PMC1408313.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () Depicts the cellular growth of the (Ts) mutant strain AA7852 separately transformed with pVH124 (ΔU, ΔV), pVH125 (U, ΔV) or pVH119 (U, V) incubated at different temperatures. Isolated colonies of the independent transformants were streaked onto LB-Ap plates and incubated overnight at the indicated temperatures. () Presents the immunodetection of Pth(Ts) in the (Ts) mutant strain AA7852 separately transformed with pVH124, pVH125, pVH119, ptRNACCA (X, R, T, M) or pTH2 (W) and grown at 32°C prior to transfer at time = 0 min at 41 or 43°C. The concentration of Pth(Ts) protein was estimated by immunoblot analysis. The left lane shows purified wild-type Pth protein, which migrates slightly faster in SDS–PAGE than the Pth(Ts) variant (arrowed) ()
Emergence of oblique TS mode due to longitudinal wall oscillation in 2D channel flow
No full textStabilizing effects of wall oscillation on two dimensional (2D) Tollmein-Schlichting (TS) waves and oblique TS waves developing in 2D channel flow are numerically investigated using the Floquet method. It was shown from the previous study[1] that the wall oscillation mostly has stabilizing effect and then TS mode is occasionally more stable than the oblique mode. In the present study the characteristics of the stability are investigated in detail. Then it is cleared that the exchange of the most unstable mode form the 2D TS to the oblique TS can occur at a certain value of Reynolds number
TS-BTPhen as a Promising Hydrophilic Complexing Agent for Selective Am(III) Separation by Solvent Extraction
No full textThe novel hydrophilic back-extraction agent TS-BTPhen (3,3’,3’’,3’’’-[3-(1,10-phenanthroline-2,9-diyl)-1,2,4-triazine-5,5,6,6-tetrayl]tetrabenzenesulfonic acid) was tested for its selectivity towards Am(III) over Cm(III) and Eu(III) with a TODGA (N,N,N’,N’-tetraoctyldiglycolamide) based solvent. Batch experiments were carried out using TS-BTPhen dissolved in aqueous nitric acid solution with tracers of 152Eu, 241Am and 244Cm. A significant increase of the separation factor for Cm over Am from SFCm/Am 1.6 to SFCm/Am 3.3 was observed compared to the use of a TODGA-nitric acid system alone. Furthermore, stripping was possible at high nitric acid concentrations (0.6 - 0.7 mol/L) resulting in a low sensitivity to acidity changes. The influence of the TS-BTPhen concentration was analysed. A slope of -2 was expected taking into account literature stoichiometries of the lipophilic analogue CyMe4BTPhen. However, a slope of -1 was found. Batch stripping kinetics showed fast kinetics for the trivalent actinides. As an alternative organic ligand the methylated TODGA derivate Me-TODGA (2-methyl-N,N,N’,N’-tetraoctyldiglycolamide) was tested in combination with the hydrophilic TS-BTPhen. The Am(III) separation was achieved at even higher nitric acid concentrations compared to TODGA
Separation of form from orientation in 3D measurements of aspheric surfaces with no datum
No full textMeasurement and characterisation of 3D form to maintain manufacturing quality has particular problems in cases such as lenses which do not generally have a clear measurement datum. A 3D-form measurement includes information about the form, the orientation and the position of the surface under test. Orientation and position can be design parameters or may result from misalignment of the test specimen on a measurement table. In either case, it is necessary to separate form from orientation and position if the data-set is to be fitted and compared with an "ideal" surface. In this paper two pre-processing algorithms are presented and examples given of the separation of form from orientation and position. The algorithm is applied to simulated data-sets consisting of up to 26,000 discrete points on a square grid, simulating the measurement of an aspheric lens in 3D. The rotationally symmetric data-set is translated for a distance x0, y0 and z0 and rotated about two axes, x and y, to simulate misalignment. To simulate inaccuracies from a manufacturing process, normally distributed random noise is superimposed on the ideal surface. An application of pre-processing using a real data-set is also shown. Furthermore, form fitting is addressed and the interpretation of form by decomposition of the data into error types is discussed
A TS uszályhíd kifejlesztése és alkalmazása
No full textThe essay focuses on the history of floating bridges and their special situation in Hungary. It produces the main elements and building procedures of the TS barge bridge. The author writes an appreciation of the TS barge bridge designers.The essay focuses on the history of floating bridges and their special situation in Hungary. It produces the main elements and building procedures of the TS barge bridge. The author writes an appreciation of the TS barge bridge designers
The analysis of the form of aspheric lens surfaces
No full textRecent developments in high precision machining have allowed for the design and manufacture of precision aspheric lenses. These have the advantage over conventional spherical lenses in that a multiple element lens can be replaced by a single aspheric lens. There are however difficulties both in the measurement of the lens surface after machining and the assessment of the quality of the lens. In this paper an aspheric lens surface is measured using a 3D Form Talysurf. The data are then analysed using an analytical least-squares technique to fit the points to an exponential function. The methods developed show that the machining methods can now be complemented by an analysis technique
The new CEN/TS 19100: Design of glass structures
No full textSince the beginning of 2021, CEN/TS 19100 Design of Glass Structures has been available in its first three parts. The fourth part is expected soon. This Technical Specification of the European standards organisation CEN is as a pre-standard of a corresponding future Eurocode. These documents constitute the first ever comprehensive design code for the entire structural glass engineering field on the European market for the first time. In addition to a clear outline, the Technical Specification has been drafted to be compatible with EN 1990 “Basis of Design” and to address glass-specific design matters, particularly related to robustness and redundancy. Although the standard still has the status of a CEN/TS, thereby allowing the European nations the option of whether to introduce it, either in full or in parts, it already contains national openings through which the European countries can adapt the design results to their own safety level by National Determined Parameters (NDPs). Such an approach already anticipates the future Eurocode, which is expected to be published as EN 19100—Design of Glass Structures. This article provides some context on the history and concept behind the new documents and gives an overview of the design rules and the corresponding technical background of the different parts of CEN/TS 19100.Applied MechanicsArchitectural Technolog
The merger of Proto-Burmish *ts and *č in Burmese
No full textAlthough Old Burmese is the earliest attested Burmish language, more recently attested kindred languages preserve archaic features which Old Burmese has lost. This paper examines one such Burmese innovation, the merger of *ts and *č
Development of EMT/TS Co-simulation Using PowerFactory and PSS/E
No full textAs the scale and complexity of power systems increase, simulating them in efficient and accurate ways continues tobe a challenge in power systems engineering. Electromagnetic Transient (EMT) and Transient Stability (TS) simulation are the two main dynamic power system simulation methods. To simulate large and complex power systems in sufficient detail without sacrificing execution time, one of the idea is to perform a co-simulation that couples EMT and TS simulator. Although several attempts have been made to couple both simulator, only on rare occasions do these hybrid EMT-TS simulators couple two or more industry-adopted simulation tools.The objective of this thesis is to to develop and study the benefits and limitations of the Electromagnetic Transient – Transient Stability co-simulation based on PowerFactory and PSS/E, both of which are among the most extensively used simulation tools in industry and academia alike. With regards to the objective, the EMT-TS co-simulation using PowerFactory and PSS/E has been developed. Then, several tests are performed to evaluate the function of each composing part of the EMT-TS co-simulation, and to test the integration between all its component. Next, The developed co-simulation is applied to study cases and the results are compared to a monolithic EMT simulation to evaluate its accuracy and execution time. Furthermore, the effect of TS and EMT time step to the accuracy and execution time of EMT-TS co-simulation have also been investigated.The study case results show that the developed EMT-TS co-simulation has not been beneficial yet in terms of accuracy and execution time. Although the active power result shows a similar tendency with the monolithic EMT result, the difference between both are visible. The difference between both are more prominent in the reactive power result. The total execution time of the developed co-simulation in the study cases are in the range of 23-24 minutes, significantly larger than the total execution time obtained from the monolithic EMT simulation which is around of 12s. Also, it is found that reducing the TS time step from 0.02 s to 0.01 s slightly increases the total simulation time from 23 to 26 minutes. However, it does not contribute a significant improvement on the accuracy of the developed EMT-TS co-simulation. The result obtained from reducing the EMT time step to is the same with the result obtained using EMT time step. Moreover, the reduction of the EMT time step significantly increases the total simulation time from 23 to 42 minutes. The developed co-simulation still has a lot of room for improvement and further developments in this topic might increase its performance.Electrical Engineering | Sustainable Energy Technolog
TS-MPC for autonomous vehicles Including a TS-MHE-UIO estimator
No full text© 2019 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.In this paper, a novel approach is presented to solve the trajectory tracking problem for autonomous vehicles. This approach is based on the use of a cascade control where the external loop solves the position control using a novel Takagi Sugeno-Model Predictive Control (TS-MPC) approach and the internal loop is in charge of the dynamic control of the vehicle using a Takagi Sugeno-Linear Quadratic Regulator technique designed via Linear Matrix Inequalities (TS-LMI-LQR). Both techniques use a TS representation of the kinematic and dynamic models of the vehicle. In addition, a novel Takagi-Sugeno estimator-Moving Horizon Estimator-Unknown Input Observer (TS-MHE-UIO) is presented. This method estimates the dynamic states of the vehicle optimally as well as the force of friction acting on the vehicle that is used to reduce the control efforts. The innovative contribution of the TS-MPC and TS-MHE-UIO techniques is that using the TS model formulation of the vehicle allows us to solve the nonlinear problem as if it were linear, reducing computation times by 10-20 times. To demonstrate the potential of the TS-MPC, we propose a comparison between three methods of solving the kinematic control problem: Using the nonlinear MPC formulation (NL-MPC) with compensated friction force, the TS-MPC approach with compensated friction force, and TS-MPC without compensated friction force.This work was supported by the Spanish Min-istry of Economy and Competitiveness (MINECO) and FEDER through theProjects SCAV (ref. DPI2017-88403-R) and HARCRICS (ref. DPI2014-58104-R). The corresponding author, Eugenio Alcalá, is supported under FI AGAURGrant (ref 2017 FI B00433).Peer ReviewedPostprint (author's final draft
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