44 research outputs found

    CHROMATOGRAPHIA on the Move

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    Sample Pretreatment Procedures

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    I am Detroit

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    30 p.The author describes the inspiration and components of a photographic installation celebrating the city of Detroit

    The Sinclair Lewis Society Newsletter, Vol. 11, No. 2

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    “Interview with Richard Lingeman,” by Sally E. Parry, Illinois State University “Lewis Catches Flivver Fever: Author Enjoyed the Early Motoring Days,” by Dave Simpkins, Sauk Centre Herald “Arrowsmith in Japanese,” by Rusty Allred “Lewis, London-and Hemingway?,” by Robert E. Fleming, University of New Mexico “Edith Wharton in Sinclair Lewis,” by Martin Bucco, Colorado State University “The Art of the Literary Feud,” rev. of Literary Feuds: A Century of Celebrated Quarrels-From Mark Twain to Tom Wolfe by Anthony Arthur; by Sally E. Parry, Illinois State University “Hemingway Read Some Lewis,” by Hilary Justice, Illinois State University “Enlightened on Lewis,” by Dave Simpkins, Sauk Centre Heraldhttps://ir.library.illinoisstate.edu/slsn/1045/thumbnail.jp

    Direct Dynamic Protein-Affinity Selection Mass-Spectrometry

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    A new methodology is described enabling the affinity screening of potential ligands towards the human estrogen receptor alpha ligand binding domain (ERα-LBD). In-solution incubation is performed of the analyte and the His-tagged ERα-LBD. The bound complex is immobilized on a nickel-loaded protein-affinity selection column, where after the unbound fraction is removed. The immobilized protein–ligand complex is exposed to a decreased pH value and an increased organic modifier concentration releasing the ligand for MS detection, and precipitating the proteins on a filter positioned between the affinity column and the mass spectrometer. The trapping column can be regenerated for reuse at least 70 times. The advantages of the methodology over existing methodologies are the absence of a pre-concentration as well as a chromatographic separation step, resulting in a significantly shorter analysis time compared to previously described procedures, and in addition, allowing the determination of solutes with unfavorable chromatographic properties. The overall analysis time now can be reduced about 250% to approximately 6 min. Replacing the filters after every measurement results in an intra-day standard deviation of 14.8% and an inter-day standard deviation of 21.3%

    The Last Titan: A Life of Theodore Dreiser. by Jerome Loving. (Berkley: University of California Press, 2005. xvi, 480 pp. $34.95, ISBN 0-520-23481-2.)

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    Theodore Dreiser, sometimes called the “Father of American Realism” (p. 140), has long occupied a contested position in American literary studies. Damaging accusations of plagiarism and anti-Semitism have diminished his reputation, yet as the author of Sister Carrie (1900) and An American Tragedy (1925), two of the most highly regarded naturalistic novels in the American literary canon, Dreiser cannot be so easily dismissed. Enter The Last Titan, Jerome Loving\u27s sweeping and meticulously researched biography of the man he calls “the last major American writer of the nineteenth century” (p. 164). The Last Titan differs from previous Dreiser bi-ographies—notably W. A. Swanberg\u27s Dreiser (1965) and Richard Lingeman\u27s two-volume Theodore Dreiser (1986, 1990)—in that Loving organizes the book around his subject\u27s prodigious literary output. Countless readers are familiar with Sister Carrie and An American Tragedy, but Dreiser also produced six other novels,..
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