881 research outputs found
SILVA_123 Eukaryota taxonomic training data formatted for DADA2; plus version with (uncurrated) additional sequences
No full text<p>This is the Silva123 taxonomy reformatted to work with eukaryote sequences for dada2:</p>
<p><a href="https://zenodo.org/api/files/4f873b5b-fe02-4344-8c8b-c723c3caba67/SILVA_123_dada2.fasta">SILVA_123_dada2.fasta </a></p>
<p>It has been created using a script available <a href="https://github.com/derele/AA_Hyena/blob/master/R/convert_silva_taxonomy.r">here</a>.</p>
<p>To increase coverage (Silva has a low coverage for eukaryotes). Uncurrated additional sequences similar to ASVs found in the intestine of hyenas (BLAST) have been added. The script used for taxonomic annotation of these sequences is available <a href="https://github.com/derele/AA_Hyena/blob/master/scripts/blast2alltax_outfmt11.pl">here</a>. The file containing these additional (uncurrated!) sequences is:</p>
<p><a href="https://zenodo.org/api/files/4f873b5b-fe02-4344-8c8b-c723c3caba67/SILVA_123_dada2_exp.fasta?versionId=78a7526a-72e0-4707-ab5c-297671b5ff13">SILVA_123_dada2_exp.fasta </a></p>
<p>This expanded file has been used for taxonomic annotation in: </p>
<p><a href="https://doi.org/10.3389/fcimb.2017.00262">Heitlinger, E., Ferreira, S., Thierer, D., Hofer, H., & East, M. L. (2017). The intestinal eukaryotic and bacterial biome of spotted hyenas: the impact of social status and age on diversity and composition. <em>Frontiers in cellular and infection microbiology</em>, <em>7</em>, 262.</a></p>This is the version used in:
Emanuel Heitlinger, Susana C. M. Ferreira, Dagmar Thierer, Heribert Hofer, and Marion L. East (2017) The Intestinal Eukaryotic and Bacterial Biome of Spotted Hyenas: The Impact of Social Status and Age on Diversity and Composition Front. Cell. Infect. Microbio; 7: 262. doi: 10.3389/fcimb.2017.0026
The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus
No full textABSTRACT: BACKGROUND: Anguillicola crassus is an economically and ecologically important parasitic nematode of eels. The native range of A. crassus is in East Asia, where it infects Anguilla japonica, the Japanese eel. A. crassus was introduced into European eels, Anguilla anguilla, 30 years ago. The parasite is more pathogenic in its new host than in its native one, and is thought to threaten the endangered An. anguilla across its range. The molecular bases for the increased pathogenicity of the nematodes in their new hosts is not known. RESULTS: A reference transcriptome was assembled for A. crassus from Roche 454 pyrosequencing data. Raw reads (756,363 total) from nematodes from An. japonica and An. anguilla hosts were filtered for likely host contaminats and ribosomal RNAs. The remaining 353,055 reads were assembled into 11,372 contigs of a high confidence assembly (spanning 6.6 Mb) and an additional 21,153 singletons and contigs of a lower confidence assembly (spanning an additional 6.2 Mb). Roughly 55% of the high condence assembly contigs were annotated with domain- or protein sequence similarity derived functional information. Sequences conserved only in nematodes, or unique to A. crassus were more likely to have secretory signal peptides. Thousands of high quality single nucleotide polymorphisms were identified, and coding polymorphism was correlated with diffrential expression between individual nematodes. Transcripts identfied as being under positive selection were enriched in peptidases. Enzymes involved in energy metabolism were enriched in the set of genes differentially expressed between European and Asian A. crassus. CONCLUSIONS: The reference transcriptome of A. crassus is of high quality, and will serve as a basis for future work on the invasion biology of this important parasite. The polymorphisms identied will provide a key tool set for analysis of population structure and identication of genes likely to be involved in increased pathogenicity in European eel hosts. The identication of peptidases under positive selection is a first step in this programme
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Emanuel Law Outlines for Evidence.
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Emanuel Law Outlines for Torts /
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A new and accurate map of the East Jndia Jslands [cartographic material] : laid down according to the latest discoveries, and agreeable to the most approved maps and charts, the whole being regulated by astron.l observations /
No full text"English miles 60 to a degree"; "No. 40"; Cartouche title.; Contains commentaries by author, in body of map. Illustration top right hand corner surrounding title cartouche.; Map of Indonesia, and including Northern Australia, Indo-China and Malaysia.; Plate no 40 extracted from: Emanuel Bowen's, A complete system of geography, London : E. Bowen, [1747?]; Relief shown pictorially.; Shows the northern most "part of New Holland".; Rex Nan Kivell Collection Map NK 1553; In Library's copy of Bowen : Complete Atlas, this map is plate No.44.New and accurate map of the East India islands [cartographic material]Complete system of geograph
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Emanuel Law Outlines for Civil Procedure.
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Emanuel Law Outlines for Criminal Procedure.
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Emanuel Law Outlines for Criminal Law.
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Emanuel Law Outlines for Corporations and Other Business Entities.
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