3,806 research outputs found
Coming home to mother
No full textGift of Dr. Mary Jane Esplen.Piano vocal [instrumentation]We love to think of years ago [first line]We are coming home to Mother [first line of chorus]A flat [key]Piano [tempo]House, families, birds, photograph of M.E. Mollins [illustration]Popular song [form/genre]Publisher's advertisement on inside front [note]Mediatoon by G.A. Boyton [note
Chlamydia trachomatis major outer membrane protein epitopes expressed as fusions with LamB in an attenuated aroA strain of Salmonella typhimurium; their application as potential immunogens
No full textThe major outer-membrane protein (MOMP) of Chlamydia trachomatis is the focus of attention for chlamydial vaccine design, particularly those serovar- and subspecies-specific epitopes which provoke neutralizing immune responses. Selected surface-exposed B-cell epitopes of MOMP, incorporating B-subspecies specificities, were expressed as fusions with LamB, an inducible outer-membrane transport protein of Escherichia coli. These recombinant chlamydial-LamB proteins were correctly transported to the outer membrane of both E. coli and an aro A mutant of Salmonella typhimurium. The immunogenicity of the constructs was investigated in a mouse model of chlamydial salpingitis. After oral immunization, recombinant S. typhimurium were recovered from the livers of mice for up to two weeks, and a serum IgG response was induced both to the Salmonella and to the inserted chlamydial epitopes. By contrast, intravenous inoculation was ineffective. Although these LamB fusions proved only weakly immunogenic, this approach should be useful for investigating the ability of attenuated S. typhimurium vaccines incorporating chlamydial epitopes to stimulate protective mucosal immunity in the mouse model of chlamydial salpingitis
Interview with M. E. Hayes
No full textRecalls childhood and schooldays at Narborough Road school in Leicester. Describes first job in clothing factory at Russell's on Eastern Boulevard, Leicester, then work at Hurst's until marriage. Talks about her father being founder member of the Independent Labour Party (ILP) in Leicester and links with the Co-operative Society. Gives details of the Co-op movement and activities of the Co-operative Guild of Women. Describes working in Co-op shop from 1941, in particular working at the High Street branch
M.E. Church exterior
No full textA monochrome postcard of the M.E. Church in Georgetown, Delaware. The church was made out of brick and had a tall tower on its corner. Writing on the right indicates that the author was having a good time. Mrs. P. H. Adkins of Milton, Delaware, received this postcard. A postmark on the back indicates that this postcard was mailed from Georgetown, Delaware. The postage stamp is a one-cent U.S. #300, Benjamin Franklin. The back of the postcard is labeled 75.00 and 0240
M.E. Church exterior
No full textA monochrome postcard of the M.E. Church in Georgetown, Delaware. The church was made out of brick and had a tall tower on its corner. Writing on the right indicates that the author was having a good time. Mrs. P. H. Adkins of Milton, Delaware, received this postcard. A postmark on the back indicates that this postcard was mailed from Georgetown, Delaware. The postage stamp is a one-cent U.S. #300, Benjamin Franklin. The back of the postcard is labeled 75.00 and 0240
A combined computational and experimental approach for the analysis of the enantioselective potential of a new macrocyclic receptor for N-protected alpha-amino acids
No full textA new macrocyclic receptor incorporating a thiourea moiety has been synthesised. Crystal structures of the macrocycle showed that the receptor has a rigid backbone but the thiourea moiety can orientate itself to bind to a DMSO solvent molecule. Force-field (MMFFs) calculations were performed to model the macrocycle and its binding properties with respect to N-protected amino acids, which were measured experimentally by NMR titration. Binding free energies were calculated by using the mode integration algorithm (MINTA) or free-energy perturbation (FEP). Excellent qualitative agreement with experiment was obtained. To further exploit the accuracy of the free-energy predictions for this system, the faster free-energy algorithm MINTA was used as a prediction tool to test the binding affinity of the macrocycle towards a series of several other amino acid derivatives, which speeded up considerably the screening process and reduced laboratory costs
On using Directional Information for Parameter Space Decomposition in Ellipse Detection
No full textIn this paper we use the parametric polar representation to extend the application of edge directional information from circle to ellipse extraction. As a result we obtain a mapping which decomposes the parameter space required for ellipse extraction into two independent sub-spaces and one final histogram accumulator. The mapping includes the tangent of the angle of the first and second directional derivatives. These tangents are computed by considering edge direction at two border points. We show that the use of gradient information for parameter space decomposition avoids the intensive point labelling imposed by geometric constraints used by other approaches
Predicting enantioselectivity: Computation as an efficient "experimental" tool for probing enantioselectivity
No full textAs a result of the accurate agreement between computation and experiment obtained using default forcefield parameters, the MMFFs forcefield together with a Monte Carlo conformational search method (MCMM/LMCS) and the MINTA free-energy calculation algorithm has been used to probe the enantioselective potential of a new macrocyclic receptor, hence saving time and money on costly experimental procedures
La fecondazione assistita dopo dieci anni di legge 40 : il punto di vista del costituzionalista
No full textThe Author of this paper adds the point of view of the constitutionalist to the debate on the Italian statute n.40/1994 on assisted reproduction illustrated by Flamigni and Mori in their work. The paper ends with a reflection on the recent decision n. 162/2014 issued by the Constitutional Court, which has declared the illegitimacy of the prohibition of heterologous reproduction
L'approvazione della legge Cirinnà : fra riconoscimento dei diritti e scontro ideologico
No full textThe New Italian Law on Civil Unions: Between Rights Recognition and Ideological Clash Abstract: The author examines the coming into force of the first Italian law on civil unions, which, for the first time, grants protection for both homosexual and different sex couples
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